北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 期刊论文
学科主题: 基础医学
题名:
Human Hepatocytes with Drug Metabolic Function Induced from Fibroblasts by Lineage Reprogramming
作者: Du, Yuanyuan1; Wang, Jinlin2; Jia, Jun1,2; Song, Nan1,2; Xiang, Chengang1; Xu, Jun1; Hou, Zhiyuan4; Su, Xiaohua1; Liu, Bei2; Jiang, Tao5; Zhao, Dongxin1; Sun, Yingli6; Shu, Jian1; Guo, Qingliang5; Yin, Ming1; Sun, Da2; Lu, Shichun5; Shi, Yan2; Deng, Hongkui1,2,3
刊名: CELL STEM CELL
发表日期: 2014-03-06
DOI: 10.1016/j.stem.2014.01.008
卷: 14, 期:3, 页:394-403
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: CELL-DERIVED HEPATOCYTES ; PANCREAS PROGENITORS ; LIVER ; DIFFERENTIATION ; MATURATION ; SYSTEMS
英文摘要:

Obtaining fully functional cell types is a major challenge for drug discovery and regenerative medicine. Currently, a fundamental solution to this key problem is still lacking. Here, we show that functional human induced hepatocytes (hiHeps) can be generated from fibroblasts by overexpressing the hepatic fate conversion factors HNF1A, HNF4A, and HNF6 along with the maturation factors ATF5, PROX1, and CEBPA. hiHeps express a spectrum of phase I and II drug-metabolizing enzymes and phase III drug transporters. Importantly, the metabolic activities of CYP3A4, CYP1A2, CYP2B6, CYP2C9, and CYP2C19 are comparable between hiHeps and freshly isolated primary human hepatocytes. Transplanted hiHeps repopulate up to 30% of the livers of Tet-uPA/Rag2(-/-)/gamma c(-/-) mice and secrete more than 300 mu g/ml human ALBUMIN in vivo. Our data demonstrate that human hepatocytes with drug metabolic function can be generated by lineage reprogramming, thus providing a cell resource for pharmaceutical applications.

语种: 英语
所属项目编号: 973 Program 2012CB966401 ; 2011AA020107 ; 2013DFG30680 ; Z121100005212001 ; 2011ZX09102-010-03 ; 1023963 ; 37871
项目资助者: National Basic Research Program of China ; Ministry of Science and Technology ; Science and Technology Commission of Beijing ; Key New Drug Creation and Manufacturing Program ; Ministry of Education of China (111 Project) ; Bill &amp ; Melinda Gates Foundation
WOS记录号: WOS:000332533400015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51504
Appears in Collections:基础医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Beijing Vitalstar Biotechnol, Beijing 100012, Peoples R China
2.Peking Univ, Peking Tsinghua Ctr Life Sci, Coll Life Sci, MOE Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China
3.Peking Univ, Shenzhen Grad Sch, Key Lab Chem Genom, Shenzhen Stem Cell Engn Lab, Shenzhen 518055, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Peking Univ Stem Cell Res Ctr,Dept Cell Biol, Beijing 100191, Peoples R China
5.Chinese Peoples Liberat Army Gen Hosp, Dept Hepatobiliary Surg, Beijing 100853, Peoples R China
6.Chinese Acad Sci, Beijing Inst Genom, Lab Genome Variat & Precis Biomed, Beijing 100101, Peoples R China

Recommended Citation:
Du, Yuanyuan,Wang, Jinlin,Jia, Jun,et al. Human Hepatocytes with Drug Metabolic Function Induced from Fibroblasts by Lineage Reprogramming[J]. CELL STEM CELL,2014,14(3):394-403.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Du, Yuanyuan]'s Articles
[Wang, Jinlin]'s Articles
[Jia, Jun]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Du, Yuanyuan]‘s Articles
[Wang, Jinlin]‘s Articles
[Jia, Jun]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  北京大学医学部 - Feedback
Powered by CSpace