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学科主题: 基础医学
题名:
A genome map of Salmonella enterica serovar Agona: numerous insertions and deletions reflecting the evolutionary history of a human pathogen
作者: Chen, Fang1; Poppe, Cornelis2; Liu, Gui-Rong1,3,4; Li, Yong-Guo5; Peng, Yi-Hong1; Sanderson, Kenneth E.4; Johnston, Randal N.6; Liu, Shu-Lin1,3,4,5
关键词: Salmonella Agona ; genomic evolution ; physical map
刊名: FEMS MICROBIOLOGY LETTERS
发表日期: 2009-04-01
DOI: 10.1111/j.1574-6968.2009.01539.x
卷: 293, 期:2, 页:188-195
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
关键词[WOS]: RESISTANT NONTYPHOIDAL SALMONELLA ; FIELD GEL-ELECTROPHORESIS ; EAT SAVOURY SNACK ; CLEAVAGE MAP ; RIBOSOMAL-RNA ; HOMOLOGOUS RECOMBINATION ; CHLAMYDOMONAS-EUGAMETOS ; ESCHERICHIA-COLI ; TYPHIMURIUM LT2 ; SEROTYPE AGONA
英文摘要:

Salmonella enterica serovar Agona is an important zoonotic pathogen, causing serious human illness worldwide, but knowledge about its genetics and evolution, especially regarding the genomic events that might have contributed to the formation of S. Agona as an important pathogen, is lacking. As a first step toward understanding this pathogen and characterizing its genomic differences with other salmonellae, we constructed a physical map of S. Agona in strain SARB1 using I-CeuI, XbaI, AvrII and Tn10 insertions with pulsed-field gel electrophoresis techniques. On the 4815-kb genomic map, we located 82 genes, revealed one inversion of about 1000 kb and resolved seven deletions and seven insertions ranging from 10 to 67 kb relative to the genome of Salmonella typhimurium LT2. These genomic features clearly distinguish S. Agona from other previously analyzed salmonellae and provide clues to the molecular basis for its genomic divergence. Additionally, these kinds of physical maps, combined with emerging high-speed sequencing technologies, such as the Solexa or SOLiD techniques, which require a pre-existing high-resolution physical map such as the S. Agona map reported here, will play important roles in genomic comparative studies of bacteria involving large numbers of strains.

语种: 英语
所属项目编号: 256177 ; NSFC30370774 ; NSFC30870098 ; 20030001029
项目资助者: Genome Canada ; National Natural Science Foundation of China ; Doctoral Program of Higher Education ; Natural Sciences and Engineering Research Council
WOS记录号: WOS:000264240900005
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51529
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Peking Univ, Dept Microbiol, Hlth Sci Ctr, Beijing 100871, Peoples R China
2.Publ Hlth Agcy Canada, Lab Foodborne Zoonoses, Guelph, ON, Canada
3.Harbin Med Coll, Genom Res Ctr, State Prov Key Labs Biomed Pharmaceut China, Harbin 150081, Peoples R China
4.Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB, Canada
5.Harbin Med Coll, Hosp 1, Dept Infect Dis, Harbin 150081, Peoples R China
6.Univ Calgary, Dept Biochem & Mol Biol, Calgary, AB, Canada

Recommended Citation:
Chen, Fang,Poppe, Cornelis,Liu, Gui-Rong,et al. A genome map of Salmonella enterica serovar Agona: numerous insertions and deletions reflecting the evolutionary history of a human pathogen[J]. FEMS MICROBIOLOGY LETTERS,2009,293(2):188-195.
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