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A simple RP-HPLC method for quantification of columbianadin in rat plasma and its application to pharmacokinetic study
Zhang, You-Bo; Yang, Xiu-Wei
关键词Angelica Pubescens F. Biserrata Columbianadin Pharmacokinetic Rp-hplc
刊名BIOMEDICAL CHROMATOGRAPHY
2010-04-01
DOI10.1002/bmc.1310
24期:4页:433-437
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]NATURAL PRODUCT ; COMPONENTS ; COUMARINS
英文摘要

A rapid and sensitive reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed to investigate pharmacokinetics of columbianadin, one of the main bioactive constituents in the roots of Angelica pubescens f. biserrata, in rat plasma after intravenous administration to rats at two doses of 10 and 20 mg/kg. The method involves a plasma clean-up step using liquid-liquid extraction by diethyl ether, followed by RP-HPLC separation and detection. Separation of columbianadin was performed on an analytical Diamonsil (TM) ODS C(18) column, with a mobile phase of MeOH-H(2)O (85:15, v/v) at a flow-rate of 1.0 mL/min, and UV detection was set at 325 nm. The retention time of columbianadin and scoparone (internal standard) was 6.7 and 3.5 min, respectively. The calibration curve was linear over the range of 0.2-20.0 mu g/mL (r(2) = 0.9986) in rat plasma. The lower limits of detection and quantification were 0.05 and 0.1 mu g/mL, respectively. The extraction recovery from plasma was in the range of 81.61-89.93%. The intra- and inter-day precisions (relative standard deviation) were between 1.01 and 9.33%, with accuracies ranging from 89.76 to 109.22%. The results indicated that the method established was suitable for the determination and pharmacokinetic study of columbianadin in rat plasma. Copyright (C) 2009 John Wiley & Sons, Ltd.

语种英语
WOS记录号WOS:000276169100014
项目编号30672609 ; 2006BAI06A01-02 ; 2002AA2Z343C ; 2004AA2Z3783 ; Z0004105040311 ; 2008-0120
资助机构National Natural Science Foundation of China ; National Sciences and Technology Program of China ; National High Technology Research and Development Program of China ; Beijing Municipal Special-Purpose Science Foundation of China ; Science Foundation of State Key Laboratory of Natural and Biomimetic Drugs of Peking University
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51550
专题北京大学药学院
北京大学药学院_天然药物学系
作者单位Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Zhang, You-Bo,Yang, Xiu-Wei. A simple RP-HPLC method for quantification of columbianadin in rat plasma and its application to pharmacokinetic study[J]. BIOMEDICAL CHROMATOGRAPHY,2010,24(4):433-437.
APA Zhang, You-Bo,&Yang, Xiu-Wei.(2010).A simple RP-HPLC method for quantification of columbianadin in rat plasma and its application to pharmacokinetic study.BIOMEDICAL CHROMATOGRAPHY,24(4),433-437.
MLA Zhang, You-Bo,et al."A simple RP-HPLC method for quantification of columbianadin in rat plasma and its application to pharmacokinetic study".BIOMEDICAL CHROMATOGRAPHY 24.4(2010):433-437.
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