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学科主题: 药学
题名:
A simple RP-HPLC method for quantification of columbianadin in rat plasma and its application to pharmacokinetic study
作者: Zhang, You-Bo; Yang, Xiu-Wei
关键词: Angelica pubescens f. biserrata ; columbianadin ; pharmacokinetic ; RP-HPLC
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2010-04-01
DOI: 10.1002/bmc.1310
卷: 24, 期:4, 页:433-437
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: NATURAL PRODUCT ; COMPONENTS ; COUMARINS
英文摘要:

A rapid and sensitive reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed to investigate pharmacokinetics of columbianadin, one of the main bioactive constituents in the roots of Angelica pubescens f. biserrata, in rat plasma after intravenous administration to rats at two doses of 10 and 20 mg/kg. The method involves a plasma clean-up step using liquid-liquid extraction by diethyl ether, followed by RP-HPLC separation and detection. Separation of columbianadin was performed on an analytical Diamonsil (TM) ODS C(18) column, with a mobile phase of MeOH-H(2)O (85:15, v/v) at a flow-rate of 1.0 mL/min, and UV detection was set at 325 nm. The retention time of columbianadin and scoparone (internal standard) was 6.7 and 3.5 min, respectively. The calibration curve was linear over the range of 0.2-20.0 mu g/mL (r(2) = 0.9986) in rat plasma. The lower limits of detection and quantification were 0.05 and 0.1 mu g/mL, respectively. The extraction recovery from plasma was in the range of 81.61-89.93%. The intra- and inter-day precisions (relative standard deviation) were between 1.01 and 9.33%, with accuracies ranging from 89.76 to 109.22%. The results indicated that the method established was suitable for the determination and pharmacokinetic study of columbianadin in rat plasma. Copyright (C) 2009 John Wiley & Sons, Ltd.

语种: 英语
所属项目编号: 30672609 ; 2006BAI06A01-02 ; 2002AA2Z343C ; 2004AA2Z3783 ; Z0004105040311 ; 2008-0120
项目资助者: National Natural Science Foundation of China ; National Sciences and Technology Program of China ; National High Technology Research and Development Program of China ; Beijing Municipal Special-Purpose Science Foundation of China ; Science Foundation of State Key Laboratory of Natural and Biomimetic Drugs of Peking University
WOS记录号: WOS:000276169100014
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51550
Appears in Collections:北京大学药学院_期刊论文

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作者单位: Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, You-Bo,Yang, Xiu-Wei. A simple RP-HPLC method for quantification of columbianadin in rat plasma and its application to pharmacokinetic study[J]. BIOMEDICAL CHROMATOGRAPHY,2010,24(4):433-437.
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