IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
Insulin-Producing Cells Derived from Human Embryonic Stem Cells: Comparison of Definitive Endoderm- and Nestin-Positive Progenitor-Based Differentiation Strategies
Wei, Rui1,3; Yang, Jin1,3; Hou, Wenfang1,3; Liu, Guoqiang1,3; Gao, Meijuan1,3; Zhang, Lin1,3; Wang, Haining1,3; Mao, Genhong2,3; Gao, Hongwei1,3; Chen, Guian2,3; Hong, Tianpei1,3
刊名PLOS ONE
2013-08-12
DOI10.1371/journal.pone.0072513
8期:8
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]PANCREATIC-ISLETS ; BETA-CELLS ; DIABETIC MICE ; IN-VITRO ; EXPRESSION ; MOUSE ; MICRORNAS ; LINES ; VIVO ; ENDOCRINE
英文摘要

Human embryonic stem cells (hESCs) are pluripotent and capable of undergoing multilineage differentiation into highly specialized cells including pancreatic islet cells. Thus, they represent a novel alternative source for targeted therapies and regenerative medicine for diabetes. Significant progress has been made in differentiating hESCs toward pancreatic lineages. One approach is based on the similarities of pancreatic beta cell and neuroepithelial development. Nestin-positive cells are selected as pancreatic beta cell precursors and further differentiated to secrete insulin. The other approach is based on our knowledge of developmental biology in which the differentiation protocol sequentially reproduces the individual steps that are known in normal beta cell ontogenesis during fetal pancreatic development. In the present study, the hESC cell line PKU1.1 was induced to differentiate into insulin-producing cells (IPCs) using both protocols. The differentiation process was dynamically investigated and the similarities and differences between both strategies were explored. Our results show that IPCs can be successfully induced with both differentiation strategies. The resulting IPCs from both protocols shared many similar features with pancreatic islet cells, but not mature, functional beta cells. However, these differently-derived IPC cell types displayed specific morphologies and different expression levels of pancreatic islet development-related markers. These data not only broaden our outlook on hESC differentiation into IPCs, but also extend the full potential of these processes for regenerative medicine in diabetes.

语种英语
WOS记录号WOS:000323097300199
项目编号2012CB517502 ; 2006AA02A112 ; 30771032 ; 30700879 ; 81070701 ; 81000315 ; 81270858 ; 20100001110083
资助机构Chinese National 973 Program ; Chinese National 863 Program ; National Natural Sciences Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51560
专题北京大学第三临床医学院
北京大学第三临床医学院_内分泌科
作者单位1.Peking Univ, Hosp 3, Dept Endocrinol & Metab, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 3, Reprod Med Ctr, Beijing 100871, Peoples R China
3.Peking Univ, Hosp 3, Clin Stem Cell Res Ctr, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Wei, Rui,Yang, Jin,Hou, Wenfang,et al. Insulin-Producing Cells Derived from Human Embryonic Stem Cells: Comparison of Definitive Endoderm- and Nestin-Positive Progenitor-Based Differentiation Strategies[J]. PLOS ONE,2013,8(8).
APA Wei, Rui.,Yang, Jin.,Hou, Wenfang.,Liu, Guoqiang.,Gao, Meijuan.,...&Hong, Tianpei.(2013).Insulin-Producing Cells Derived from Human Embryonic Stem Cells: Comparison of Definitive Endoderm- and Nestin-Positive Progenitor-Based Differentiation Strategies.PLOS ONE,8(8).
MLA Wei, Rui,et al."Insulin-Producing Cells Derived from Human Embryonic Stem Cells: Comparison of Definitive Endoderm- and Nestin-Positive Progenitor-Based Differentiation Strategies".PLOS ONE 8.8(2013).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wei, Rui]的文章
[Yang, Jin]的文章
[Hou, Wenfang]的文章
百度学术
百度学术中相似的文章
[Wei, Rui]的文章
[Yang, Jin]的文章
[Hou, Wenfang]的文章
必应学术
必应学术中相似的文章
[Wei, Rui]的文章
[Yang, Jin]的文章
[Hou, Wenfang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。