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学科主题: 临床医学
题名:
Insulin-Producing Cells Derived from Human Embryonic Stem Cells: Comparison of Definitive Endoderm- and Nestin-Positive Progenitor-Based Differentiation Strategies
作者: Wei, Rui1,3; Yang, Jin1,3; Hou, Wenfang1,3; Liu, Guoqiang1,3; Gao, Meijuan1,3; Zhang, Lin1,3; Wang, Haining1,3; Mao, Genhong2,3; Gao, Hongwei1,3; Chen, Guian2,3; Hong, Tianpei1,3
刊名: PLOS ONE
发表日期: 2013-08-12
DOI: 10.1371/journal.pone.0072513
卷: 8, 期:8
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: PANCREATIC-ISLETS ; BETA-CELLS ; DIABETIC MICE ; IN-VITRO ; EXPRESSION ; MOUSE ; MICRORNAS ; LINES ; VIVO ; ENDOCRINE
英文摘要:

Human embryonic stem cells (hESCs) are pluripotent and capable of undergoing multilineage differentiation into highly specialized cells including pancreatic islet cells. Thus, they represent a novel alternative source for targeted therapies and regenerative medicine for diabetes. Significant progress has been made in differentiating hESCs toward pancreatic lineages. One approach is based on the similarities of pancreatic beta cell and neuroepithelial development. Nestin-positive cells are selected as pancreatic beta cell precursors and further differentiated to secrete insulin. The other approach is based on our knowledge of developmental biology in which the differentiation protocol sequentially reproduces the individual steps that are known in normal beta cell ontogenesis during fetal pancreatic development. In the present study, the hESC cell line PKU1.1 was induced to differentiate into insulin-producing cells (IPCs) using both protocols. The differentiation process was dynamically investigated and the similarities and differences between both strategies were explored. Our results show that IPCs can be successfully induced with both differentiation strategies. The resulting IPCs from both protocols shared many similar features with pancreatic islet cells, but not mature, functional beta cells. However, these differently-derived IPC cell types displayed specific morphologies and different expression levels of pancreatic islet development-related markers. These data not only broaden our outlook on hESC differentiation into IPCs, but also extend the full potential of these processes for regenerative medicine in diabetes.

语种: 英语
所属项目编号: 2012CB517502 ; 2006AA02A112 ; 30771032 ; 30700879 ; 81070701 ; 81000315 ; 81270858 ; 20100001110083
项目资助者: Chinese National 973 Program ; Chinese National 863 Program ; National Natural Sciences Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education
WOS记录号: WOS:000323097300199
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51560
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Endocrinol & Metab, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 3, Reprod Med Ctr, Beijing 100871, Peoples R China
3.Peking Univ, Hosp 3, Clin Stem Cell Res Ctr, Beijing 100871, Peoples R China

Recommended Citation:
Wei, Rui,Yang, Jin,Hou, Wenfang,et al. Insulin-Producing Cells Derived from Human Embryonic Stem Cells: Comparison of Definitive Endoderm- and Nestin-Positive Progenitor-Based Differentiation Strategies[J]. PLOS ONE,2013,8(8).
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