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学科主题基础医学
Cell-based screening and validation of human novel genes associated with cell viability
Wang, Lan1; Gao, Xia1; Gao, Peng1; Deng, Weiwei1; Yu, Peng1; Ma, Jinjing1; Guo, Jinhai1; Wang, Xinyu1; Cheng, Hualing1; Zhang, Chenying1; Yu, Chuanfei1; Ma, Xi1; Lv, Bingfeng1; Lu, Yang1; Shi, Taiping1; Ma, Dalong1
关键词high-throughput screening fluorescence cell viability novel gene imaging
刊名JOURNAL OF BIOMOLECULAR SCREENING
2006-06-01
DOI10.1177/1087057106286654
11期:4页:369-376
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry
关键词[WOS]FLOW-CYTOMETRY ; APOPTOSIS ; DEATH ; ASSAYS ; OVEREXPRESSION ; IDENTIFICATION ; EXPRESSION ; PATHWAYS ; DATABASE ; TISSUES
英文摘要

In the present study, a cell-based high-throughput assay is established to identify novel human genes associated with cell viability. The assay relies on the down-regulation of Renilla luciferase (pRL) activity in a 96-well format. In addition, 2-color fluorescence probes were used to distinguish living and dead cells. As the positive control, the authors used the expression vectors encoding Bax, TNFRSFIA, and TAJ, which were widely known to effectively induce programmed cell death. They screened 409 novel genes (including alternative mRNA splicing forms) cloned in their laboratory and found that 39 genes could significantly down-regulate pRL activity. A subsequent fluorescence-based assay revealed that 4 of the 39 genes (PIP5KL1, OLFM1, RNF122, FAM26B) were associated with cell viability. Further function assays validated that the 4 genes were able to induce both necrosis and apoptosis. These results therefore indicate that a rapid and effective screening system has been developed, which should shed light on some functions of novel genes.

语种英语
WOS记录号WOS:000238391000004
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51574
专题基础医学院_免疫学系
作者单位1.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China
2.Peking Univ, Lab Med Immunol, Sch Basic Med Sci, Hlth Sci Ctr, Beijing 100871, Peoples R China
3.Peking Univ, Human Dis Genom Ctr, Beijing 100871, Peoples R China
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GB/T 7714
Wang, Lan,Gao, Xia,Gao, Peng,et al. Cell-based screening and validation of human novel genes associated with cell viability[J]. JOURNAL OF BIOMOLECULAR SCREENING,2006,11(4):369-376.
APA Wang, Lan.,Gao, Xia.,Gao, Peng.,Deng, Weiwei.,Yu, Peng.,...&Ma, Dalong.(2006).Cell-based screening and validation of human novel genes associated with cell viability.JOURNAL OF BIOMOLECULAR SCREENING,11(4),369-376.
MLA Wang, Lan,et al."Cell-based screening and validation of human novel genes associated with cell viability".JOURNAL OF BIOMOLECULAR SCREENING 11.4(2006):369-376.
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