IR@PKUHSC  > 北京大学基础医学院  > 北京大学干细胞研究中心
学科主题基础医学
The infarcted cardiac microenvironment cannot selectively promote embryonic stem cell differentiation into cardiomyocytes
Chen, You-Ren2; Li, Yang3; Chen, Li4; Yang, Xin-Chun1; Su, Pi-Xiong1; Cai, Jun1
关键词Embryonic stem cells Differentiation Microenvironment Myocardial infarction Transplantation
刊名CARDIOVASCULAR PATHOLOGY
2011-03-01
DOI10.1016/j.carpath.2009.12.003
20期:2页:77-83
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cardiac & Cardiovascular Systems ; Pathology
研究领域[WOS]Cardiovascular System & Cardiology ; Pathology
关键词[WOS]GREEN FLUORESCENT PROTEIN ; MYOCARDIAL-INFARCTION ; IMMUNE-RESPONSE ; T-LYMPHOCYTE ; HEART ; TRANSPLANTATION ; INDUCTION
英文摘要

Postinfarct congestive heart failure is one of the leading causes of morbidity and mortality in industrialized countries. It is controversial whether embryonic stern cells are feasible sources for in situ cardiac regeneration in infarcted hearts. In order to investigate whether the infarcted cardiac microenvironment could selectively promote embryonic stern cell differentiation into cardiomyocytes, we assessed the cardiac differentiation potential of mouse embryonic stern cells (mESCs) injected into normal (n=16) or acutely infarcted rat hearts (n=18). We found that the transplanted 4′,6-diamidino-2-phenylindole (DAPI)-labeled mESCs were able to survive and form stable intracardiac grafts both in normal and infarcted hearts, along with macrophages found specifically in the engraftment area. Two to four weeks after mESC transplantation, we found that more DAPI-positive mESCs differentiated into cardiomyocytes, marked by cardiac troponin T (cTnT), in normal than those in infarcted hearts (2.67+/-0.79% vs. 1.06+/-0.52%, P<.01). However, the discrepancy between the percentage of DAPI-positive cells that express cTnT in normal and that in infarcted hearts was diminished after 4 weeks (1.17+/-0.98% vs. 1.07+/-1.02%, P>.05), when the transverse striation began to present in the mESCs-derived cardiomyocytes. In addition, mESCs differentiated into vimentin-positive cardiac fibroblasts in normal and infracted hearts. Our results indicated that transplanted mESCs cannot only survive but differentiate into cardiomyocytes in infarcted rat hearts. However, the infarcted cardiac microenvironment cannot selectively promote mESCs differentiation into cardiomyocytes. (C) 2011 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000287385600002
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51578
Collection北京大学基础医学院_北京大学干细胞研究中心
作者单位1.Capital Med Univ, Dept Cardiol, Chaoyang Hosp, Beijing 100020, Peoples R China
2.Shantou Univ, Dept Cardiol, Coll Med, Affiliated Hosp 2, Shantou 515041, Peoples R China
3.Peking Univ, Stem Cell Res Ctr, Hlth Sci Ctr, Beijing 100191, Peoples R China
4.Texas A&M Univ Hlth Sci Ctr, Ctr Mol Dev & Dis, Inst Biosci & Technol, Houston, TX 77030 USA
Recommended Citation
GB/T 7714
Chen, You-Ren,Li, Yang,Chen, Li,et al. The infarcted cardiac microenvironment cannot selectively promote embryonic stem cell differentiation into cardiomyocytes[J]. CARDIOVASCULAR PATHOLOGY,2011,20(2):77-83.
APA Chen, You-Ren,Li, Yang,Chen, Li,Yang, Xin-Chun,Su, Pi-Xiong,&Cai, Jun.(2011).The infarcted cardiac microenvironment cannot selectively promote embryonic stem cell differentiation into cardiomyocytes.CARDIOVASCULAR PATHOLOGY,20(2),77-83.
MLA Chen, You-Ren,et al."The infarcted cardiac microenvironment cannot selectively promote embryonic stem cell differentiation into cardiomyocytes".CARDIOVASCULAR PATHOLOGY 20.2(2011):77-83.
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