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学科主题: 药学
题名:
Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry
作者: Zeng, Feng1,2; Wang, Wei3,4; Guan, Shuhong2; Cheng, Chunru2; Yang, Min2; Avula, Bharathi5; Khan, Ikhlas A.5; Guo, De-an1,2
关键词: Tripterygium wilfordii ; sesquiterpenoid alkaloids ; diterpenoids ; triterpenoids ; HPLC-ESI-MS ; multiple reaction monitoring ; Celastraceae
刊名: PLANTA MEDICA
发表日期: 2013-06-01
DOI: 10.1055/s-0032-1328596
卷: 79, 期:9, 页:797-805
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]: Plant Sciences ; Pharmacology & Pharmacy
关键词[WOS]: SOLID-PHASE EXTRACTION ; CHINESE HERBAL REMEDY ; THUNDER GOD VINE ; NF-KAPPA-B ; HOOK-F ; ROOT EXTRACTS ; TRIPTOLIDE ; EXPRESSION ; SUPPRESSION ; ALKALOIDS
英文摘要:

A liquid chromatography-electrospray ionization-mass spectrometry method with multiple reaction monitoring was established for simultaneous quantification of 18 bioactive constituents from the stem and root of Tripterygium wilfordii Hook. f. collected from different places in China and various commercial preparations. The chromatographic separations were achieved on an Agilent Poroshell SB-C-18 column (150 x 4.6 mm, 3.5 mu m) with gradient elution using acetonitrile and 0.03% formic acid aqueous solution in 45 min. Detection was performed in the positive ionization mode by monitoring the precursor-product combination. The validation of the method included tests of linearity, sensitivity, precision, repeatability, stability, and accuracy. All calibration curves showed good linearity (r > 0.9990) within the test range. The established method showed good precision and accuracy with intra-day and interday variations of 2-5% and 1-4%, respectively, and recoveries of 95.5-104.5%.

语种: 英语
所属项目编号: 985-2-063-112 ; 104218
项目资助者: program for Changjiang Scholars and Innovation Team in University ; Ministry of Education of China ; Global Research Network for Medicinal Plants (GRNMP) ; King Saud University
WOS记录号: WOS:000320451500013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51579
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Inst Biol Sci,Shanghai Inst Mat Med, Shanghai, Peoples R China
3.Hunan Univ Chinese Med, Sch Pharm, TCM, Changsha 410208, Hunan, Peoples R China
4.Hunan Univ Chinese Med, Sch Pharm, Ethnomed Innovat & Dev Lab, Changsha 410208, Hunan, Peoples R China
5.Univ Mississippi, Pharmaceut Sci Res Inst, Natl Ctr Nat Prod Res, Sino US TCM Res Ctr, University, MS 38677 USA

Recommended Citation:
Zeng, Feng,Wang, Wei,Guan, Shuhong,et al. Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry[J]. PLANTA MEDICA,2013,79(9):797-805.
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