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Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry
Zeng, Feng1,2; Wang, Wei3,4; Guan, Shuhong2; Cheng, Chunru2; Yang, Min2; Avula, Bharathi5; Khan, Ikhlas A.5; Guo, De-an1,2
关键词Tripterygium Wilfordii Sesquiterpenoid Alkaloids Diterpenoids Triterpenoids Hplc-esi-ms Multiple Reaction Monitoring Celastraceae
刊名PLANTA MEDICA
2013-06-01
DOI10.1055/s-0032-1328596
79期:9页:797-805
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]Plant Sciences ; Pharmacology & Pharmacy
关键词[WOS]SOLID-PHASE EXTRACTION ; CHINESE HERBAL REMEDY ; THUNDER GOD VINE ; NF-KAPPA-B ; HOOK-F ; ROOT EXTRACTS ; TRIPTOLIDE ; EXPRESSION ; SUPPRESSION ; ALKALOIDS
英文摘要

A liquid chromatography-electrospray ionization-mass spectrometry method with multiple reaction monitoring was established for simultaneous quantification of 18 bioactive constituents from the stem and root of Tripterygium wilfordii Hook. f. collected from different places in China and various commercial preparations. The chromatographic separations were achieved on an Agilent Poroshell SB-C-18 column (150 x 4.6 mm, 3.5 mu m) with gradient elution using acetonitrile and 0.03% formic acid aqueous solution in 45 min. Detection was performed in the positive ionization mode by monitoring the precursor-product combination. The validation of the method included tests of linearity, sensitivity, precision, repeatability, stability, and accuracy. All calibration curves showed good linearity (r > 0.9990) within the test range. The established method showed good precision and accuracy with intra-day and interday variations of 2-5% and 1-4%, respectively, and recoveries of 95.5-104.5%.

语种英语
WOS记录号WOS:000320451500013
项目编号985-2-063-112 ; 104218
资助机构program for Changjiang Scholars and Innovation Team in University ; Ministry of Education of China ; Global Research Network for Medicinal Plants (GRNMP) ; King Saud University
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51579
专题北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Inst Biol Sci,Shanghai Inst Mat Med, Shanghai, Peoples R China
3.Hunan Univ Chinese Med, Sch Pharm, TCM, Changsha 410208, Hunan, Peoples R China
4.Hunan Univ Chinese Med, Sch Pharm, Ethnomed Innovat & Dev Lab, Changsha 410208, Hunan, Peoples R China
5.Univ Mississippi, Pharmaceut Sci Res Inst, Natl Ctr Nat Prod Res, Sino US TCM Res Ctr, University, MS 38677 USA
推荐引用方式
GB/T 7714
Zeng, Feng,Wang, Wei,Guan, Shuhong,et al. Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry[J]. PLANTA MEDICA,2013,79(9):797-805.
APA Zeng, Feng.,Wang, Wei.,Guan, Shuhong.,Cheng, Chunru.,Yang, Min.,...&Guo, De-an.(2013).Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry.PLANTA MEDICA,79(9),797-805.
MLA Zeng, Feng,et al."Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry".PLANTA MEDICA 79.9(2013):797-805.
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