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学科主题: 基础医学
题名:
B-MYB delays cell aging by repressing p16 (INK4 alpha) transcription
作者: Huang, Yu1,2; Wu, Junfeng1,3; Li, Renzhong1,3; Wang, Peichang1,3; Han, Limin1,3; Zhang, Zongyu1,3; Tong, Tanjun1,3
关键词: p16(INK4 alpha) ; B-MYB ; Senescence ; Cell aging ; Transcriptional regulation
刊名: CELLULAR AND MOLECULAR LIFE SCIENCES
发表日期: 2011-03-01
DOI: 10.1007/s00018-010-0501-9
卷: 68, 期:5, 页:893-901
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: HUMAN-DIPLOID FIBROBLASTS ; P16(INK4A) EXPRESSION ; INHIBITOR P16(INK4A) ; GROWTH-FACTOR ; C-MYB ; SENESCENCE ; PROTEINS ; DNA ; PROLIFERATION ; OVEREXPRESSION
英文摘要:

p16 (INK4 alpha) , an inhibitor of cyclin-dependent kinase 4 and 6, has been proposed to play an important role in cellular aging and in premature senescence. The expression of the p16 (INK4 alpha) is primarily under transcriptional control. Our previous data showed that a negative regulation element lies in its promoter. In that element, a MYB-binding site (MBS) was uncovered by transcription analysis. Here, we report that MBS is a negative regulation element and B-MYB binds to this site in vivo. In human embryonic lung fibroblast cells, B-MYB downregulated p16 (INK4 alpha) expression, whereas knocking down of B-MYB upregulated it. Evidence also showed that overexpression of B-MYB in cells could increase the number of utmost passage and decrease G1 block, whereas knocking down of B-MYB could impair their replicative ability. This study provides evidence of the capacity of B-MYB not only to regulate p16 (INK4 alpha) expression but also the phenotypic consequence on cellular senescence.

语种: 英语
所属项目编号: 2007CB507400 ; 30800611
项目资助者: National Basic Research Program of China ; National Natural Science Foundation of China
WOS记录号: WOS:000287243400011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51584
Appears in Collections:基础医学院_北京大学衰老研究中心_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Res Ctr Aging, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Med Genet, Beijing 100083, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China

Recommended Citation:
Huang, Yu,Wu, Junfeng,Li, Renzhong,et al. B-MYB delays cell aging by repressing p16 (INK4 alpha) transcription[J]. CELLULAR AND MOLECULAR LIFE SCIENCES,2011,68(5):893-901.
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