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学科主题: 公共卫生
题名:
cDNA cloning and expression analysis of mouse gene encoding the protein Ercc61 which is a novel member of SNF2 family
作者: Chen, XG1; Li, Y1; Zang, MX1; Pei, XR1; Xu, YJ1; Gao, LF1
关键词: Ercc6l ; gene cloning ; SNF2 protein ; expression pattern ; subcellular localization
刊名: PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2004-05-01
卷: 31, 期:5, 页:443-448
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: EXCISION-REPAIR GENES ; RNA HELICASES ; IDENTIFICATION ; SUPERFAMILY
英文摘要:

SNF2 family includes proteins essential to genome replication, repair, and expression. The cDNA cloning and initial characterizal ion of a novel mouse member of this protein family was reported, and the new member was designated as Ercc6l (excision repair cross-complementing rodent repair deficiency, complementation group 6-like) by MGNC. The eDNA of Ercc6l was 4 002 bp and firstly cloned in silico and then identified by RT-PCR in mouse embryo heart. This gene was composed of 2 extions and mapped to mouse chromosome X. The longest ORF encoded a putative protein of 1240 amino acids. Eight highly conserved sequence regions of SNF2 protein were present in the deduced protein. Alignment against members of SNF2 family initially placed Ercc6l into ERCC6 subfamily of SNF2 family. EGFP-tagged Ercc6l protein was localized in cytoplasm of HeLa, NIH3T3 and B16 cells. BLAST search in EST database retrieved 69 homologous ESTs of Ercc6l, most of which were from mouse embryonic and tumorous tissues. RT-PCR performed on a panel of different mouse tissues demonstrated that Ercc6l strongly expressed in mouse embryo but significantly downregulated in neonate and adult normal tissue. These results suggest that Ercc6l is a novel member of SNF2 family and may be crucial for development of embryo and tumour.

语种: 中文
WOS记录号: WOS:000222914300011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51586
Appears in Collections:北京大学公共卫生学院_期刊论文

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作者单位: 1.Xihua Univ, Dept Bioengn, Chengdu 610039, Peoples R China
2.Peking Univ, Lab Mol Tox & Dev Mol Biol, Dept Nutr, Sch Publ Hlth, Beijing 100083, Peoples R China

Recommended Citation:
Chen, XG,Li, Y,Zang, MX,et al. cDNA cloning and expression analysis of mouse gene encoding the protein Ercc61 which is a novel member of SNF2 family[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2004,31(5):443-448.
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