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cDNA cloning and expression analysis of mouse gene encoding the protein Ercc61 which is a novel member of SNF2 family
Chen, XG1; Li, Y1; Zang, MX1; Pei, XR1; Xu, YJ1; Gao, LF1
关键词Ercc6l Gene Cloning Snf2 Protein Expression Pattern Subcellular Localization
刊名PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
2004-05-01
31期:5页:443-448
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]EXCISION-REPAIR GENES ; RNA HELICASES ; IDENTIFICATION ; SUPERFAMILY
英文摘要

SNF2 family includes proteins essential to genome replication, repair, and expression. The cDNA cloning and initial characterizal ion of a novel mouse member of this protein family was reported, and the new member was designated as Ercc6l (excision repair cross-complementing rodent repair deficiency, complementation group 6-like) by MGNC. The eDNA of Ercc6l was 4 002 bp and firstly cloned in silico and then identified by RT-PCR in mouse embryo heart. This gene was composed of 2 extions and mapped to mouse chromosome X. The longest ORF encoded a putative protein of 1240 amino acids. Eight highly conserved sequence regions of SNF2 protein were present in the deduced protein. Alignment against members of SNF2 family initially placed Ercc6l into ERCC6 subfamily of SNF2 family. EGFP-tagged Ercc6l protein was localized in cytoplasm of HeLa, NIH3T3 and B16 cells. BLAST search in EST database retrieved 69 homologous ESTs of Ercc6l, most of which were from mouse embryonic and tumorous tissues. RT-PCR performed on a panel of different mouse tissues demonstrated that Ercc6l strongly expressed in mouse embryo but significantly downregulated in neonate and adult normal tissue. These results suggest that Ercc6l is a novel member of SNF2 family and may be crucial for development of embryo and tumour.

语种中文
WOS记录号WOS:000222914300011
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51586
Collection北京大学公共卫生学院
作者单位1.Xihua Univ, Dept Bioengn, Chengdu 610039, Peoples R China
2.Peking Univ, Lab Mol Tox & Dev Mol Biol, Dept Nutr, Sch Publ Hlth, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Chen, XG,Li, Y,Zang, MX,et al. cDNA cloning and expression analysis of mouse gene encoding the protein Ercc61 which is a novel member of SNF2 family[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2004,31(5):443-448.
APA Chen, XG,Li, Y,Zang, MX,Pei, XR,Xu, YJ,&Gao, LF.(2004).cDNA cloning and expression analysis of mouse gene encoding the protein Ercc61 which is a novel member of SNF2 family.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,31(5),443-448.
MLA Chen, XG,et al."cDNA cloning and expression analysis of mouse gene encoding the protein Ercc61 which is a novel member of SNF2 family".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 31.5(2004):443-448.
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