IR@PKUHSC  > 北京大学口腔医学院
学科主题口腔医学
Trend of telomerase activity change during human iPSC self-renewal and differentiation revealed by a quartz crystal microbalance based assay
Zhou, Yitian1; Zhou, Ping2,3; Xin, Yinqiang1; Wang, Jie1; Zhu, Zhiqiang1; Hu, Ji4; Wei, Shicheng2,3; Ma, Hongwei1
刊名SCIENTIFIC REPORTS
2014-11-10
DOI10.1038/srep06978
4
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者Chinese Academy of Sciences ; NSFC ; Peking University ; Chinese Academy of Sciences ; NSFC ; Peking University
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]REPEAT AMPLIFICATION PROTOCOL ; SINGLE-STRANDED-DNA ; HYBRIDIZATION PROTECTION ASSAY ; PLURIPOTENT STEM-CELLS ; IMMORTAL CELLS ; BIOSENSORS ; CANCER ; LENGTH
英文摘要

Telomerase plays an important role in governing the life span of cells for its capacity to extend telomeres. As high activity of telomerase has been found in stem cells and cancer cells specifically, various methods have been developed for the evaluation of telomerase activity. To overcome the time-consuming procedures and complicated manipulations of existing methods, we developed a novel method named Telomeric Repeat Elongation Assay based on Quartz crystal microbalance (TREAQ) to monitor telomerase activity during the self-renewal and differentiation of human induced pluripotent stem cells (hiPSCs). TREAQ results indicated hiPSCs possess invariable telomerase activity for 11 passages on Matrigel and a steady decline of telomerase activity when differentiated for different periods, which is confirmed with existing golden standard method. The pluripotency of hiPSCs during differentiation could be estimated through monitoring telomerase activity and compared with the expression levels of markers of pluripotency gene via quantitative real time PCR. Regular assessment for factors associated with pluripotency or stemness was expensive and requires excessive sample consuming, thus TREAQ could be a promising alternative technology for routine monitoring of telomerase activity and estimate the pluripotency of stem cells.

语种英语
所属项目编号KJZD-EW-J01 ; 21174161 ; 21105112
资助者Chinese Academy of Sciences ; NSFC ; Peking University ; Chinese Academy of Sciences ; NSFC ; Peking University
WOS记录号WOS:000344759400002
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51598
Collection北京大学口腔医学院
作者单位1.Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Div Nanobiomed, Suzhou 215123, Peoples R China
2.Peking Univ, Sch Stomatol, Lab Interdisciplinary Studies, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
3.Peking Univ, Acad Adv Interdisciplinary Studies, Ctr Biomed Mat & Tissue Engn, Beijing 100871, Peoples R China
4.Soochow Univ, Affiliated Hosp 2, Div Endocrinol, Suzhou 215000, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Yitian,Zhou, Ping,Xin, Yinqiang,et al. Trend of telomerase activity change during human iPSC self-renewal and differentiation revealed by a quartz crystal microbalance based assay[J]. SCIENTIFIC REPORTS,2014,4.
APA Zhou, Yitian.,Zhou, Ping.,Xin, Yinqiang.,Wang, Jie.,Zhu, Zhiqiang.,...&Ma, Hongwei.(2014).Trend of telomerase activity change during human iPSC self-renewal and differentiation revealed by a quartz crystal microbalance based assay.SCIENTIFIC REPORTS,4.
MLA Zhou, Yitian,et al."Trend of telomerase activity change during human iPSC self-renewal and differentiation revealed by a quartz crystal microbalance based assay".SCIENTIFIC REPORTS 4(2014).
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