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Identification of novel alternative splicing variants of interferon regulatory factor 3
Li, Yong; Hu, Xiuhua; Song, Yuqin; Lu, Zheming; Ning, Tao; Cai, Hong; Ke, Yang
关键词Irf-3 Irf-3a Ifn Beta Splicing Variant Cancer
刊名BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
2011-03-01
DOI10.1016/j.bbagrm.2011.01.006
1809期:3页:166-175
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]STIMULATED RESPONSE ELEMENT ; GENE-EXPRESSION ; HUMAN-PAPILLOMAVIRUS ; TRANSCRIPTION FACTORS ; IRF-3 ACTIVATION ; VIRUS-INFECTION ; DNA-BINDING ; CANCER ; COMPLEX ; PROTEIN
英文摘要

Interferon regulatory factor 3 (IRF-3) plays a crucial role in host defense against viral and microbial infection as well as in cell growth regulation. IRF-3a is the only structurally and functionally characterized IRF-3 splicing variant and has been established to antagonize IRF-3 activity. Here, five novel splicing variants of IRF-3, referred to as IRF-3b, -3c, -3d, -3e, and -3f, were identified and shown to be generated by deletion of exons 2, 3, or 6 or some combination thereof. RT-PCR examination revealed that these novel splicing variants were more frequently expressed in human liver, esophagus, and cervical tumor tissues than in their normal counterparts. Additionally, electrophoretic mobility shift assay and subcellular localization showed only IRF-3 and IRF-3e were capable of binding the PRDI/III element of interferon-beta (IFN beta) promoter in vitro and underwent cytoplasm-to-nucleus translocation following Poly(I:C) stimulation. Coimmunoprecipitation assay revealed that only IRF-3c (3f) of novel splicing variants associated with IRF-3 in vivo. Further luciferase assay showed IRF-3c (3f) and IRF-3e failed to transactivate PRDI/III-containing promoter but appeared to inhibit transactivation potential of IRF-3 to varying degrees. Taken together, our findings suggest novel splicing variants may function as negative modulators of IRF-3 and may be correlated with pathogenesis of human tumors. (C) 2011 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000289138200002
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51600
专题北京大学临床肿瘤学院
北京大学临床肿瘤学院_淋巴肿瘤内科
北京大学临床肿瘤学院_病因学研究室
北京大学临床肿瘤学院_遗传学研究室
作者单位Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst,Dept Genet, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China
推荐引用方式
GB/T 7714
Li, Yong,Hu, Xiuhua,Song, Yuqin,et al. Identification of novel alternative splicing variants of interferon regulatory factor 3[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS,2011,1809(3):166-175.
APA Li, Yong.,Hu, Xiuhua.,Song, Yuqin.,Lu, Zheming.,Ning, Tao.,...&Ke, Yang.(2011).Identification of novel alternative splicing variants of interferon regulatory factor 3.BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS,1809(3),166-175.
MLA Li, Yong,et al."Identification of novel alternative splicing variants of interferon regulatory factor 3".BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS 1809.3(2011):166-175.
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