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学科主题口腔医学
Effect of Nanostructure of Mineralized Collagen Scaffolds on Their Physical Properties and Osteogenic Potential
Liu, Yan1; Luo, Dan2,3; Liu, Shuai1; Fu, Yu1; Kou, Xiaoxing1; Wang, Xuedong1; Sha, Yinlin2,3; Gan, Yehua4; Zhou, Yanheng1
关键词Nanostructure Intrafibrillarly-mineralized Collagen Extrafibrillarly-mineralized Collagen Scaffold Tissue Engineering
刊名JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
2014-06-01
DOI10.1166/jbn.2014.1794
10期:6页:1049-1060
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Nanoscience & Nanotechnology ; Materials Science, Biomaterials
研究领域[WOS]Science & Technology - Other Topics ; Materials Science
关键词[WOS]TISSUE ENGINEERING APPLICATIONS ; TURKEY TENDON COLLAGEN ; IN-VITRO ; COMPOSITE SCAFFOLDS ; HUMAN BONE ; HYDROXYAPATITE/COLLAGEN COMPOSITE ; BIOACTIVE GLASS ; COLLOIDAL GELS ; MATRIX PROTEIN ; STEM-CELLS
英文摘要

Tissue engineering has enabled development of nanostructured collagen scaffolds to meet current challenges in regeneration of lost bone. In this study, extrafibrillarly-mineralized and intrafibrillarly-mineralized collagen scaffolds were fabricated separately by a conventional crystallization method and a biomimetic, bottom-up crystallization method. Atomic force microscopy (AFM) was employed to examine the nanotopography and nanomechanics of the mineralized collagen scaffolds. The in vitro cell responses to the surface of the mineralized collagen scaffolds were analyzed by laser scanning microscope and field emission scanning electron microscopy. AFM imaging showed that these two mineralized collagen scaffolds exhibited different nanostructure, including the size, morphology and location of the apatites in collagen fibrils. The nanomechanical testing demonstrated that the intrafibrillarly-mineralized collagen scaffold, with bone-like hierarchy, featured a significantly increased Young′s modulus compared with the extrafibrillarly-mineralized collagen scaffold in both dry and wet conditions. However, these two mineralized collagen scaffolds had a similar thermal behavior. From the cell culture experiments, the intrafibrillarly-mineralized collagen scaffold showed higher cell proliferation and alkaline phosphatase activity than the extrafibrillarly-mineralized collagen scaffold. The utmost significance of this study is that the nanostructure of the mineralized collagen scaffolds can affect the initial cell adhesion, morphology and further osteogenic potential. The present study will help us to fabricate novel biomaterials for bone grafting and tissue engineering applications.

语种英语
WOS记录号WOS:000328729400014
项目编号2010DFB32980 ; 2013DFB30306 ; 81201198 ; 30973360
资助机构Projects of International Cooperation and Exchanges ; National Science Foundations of China ; Peking-Tsinghua Center for Life Sciences
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51601
专题北京大学口腔医学院_牙周科
北京大学基础医学院
北京大学第三临床医学院_神经内科
北京大学口腔医学院_口腔正畸科
北京大学口腔医学院_中心实验室
作者单位1.Peking Univ, Biomed X Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Ctr Craniofacial Stem Cell Res & Regenerat, Dept Orthodont, Beijing 100081, Peoples R China
3.Peking Univ, Single Mol & Nanobiol Lab, Dept Biophys, Sch Basic Med Sci, Beijing 100191, Peoples R China
4.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Cent Lab, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Liu, Yan,Luo, Dan,Liu, Shuai,et al. Effect of Nanostructure of Mineralized Collagen Scaffolds on Their Physical Properties and Osteogenic Potential[J]. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,2014,10(6):1049-1060.
APA Liu, Yan.,Luo, Dan.,Liu, Shuai.,Fu, Yu.,Kou, Xiaoxing.,...&Zhou, Yanheng.(2014).Effect of Nanostructure of Mineralized Collagen Scaffolds on Their Physical Properties and Osteogenic Potential.JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,10(6),1049-1060.
MLA Liu, Yan,et al."Effect of Nanostructure of Mineralized Collagen Scaffolds on Their Physical Properties and Osteogenic Potential".JOURNAL OF BIOMEDICAL NANOTECHNOLOGY 10.6(2014):1049-1060.
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