IR@PKUHSC  > 北京大学第三临床医学院  > 运动医学研究所
学科主题临床医学
Repair of full-thickness cartilage defects with cells of different origin in a rabbit model
Yan, Hui; Yu, Changlong
关键词Full-thickness Repair Cell Transplantation Chondrocytes Mscs (Mesenchymal Stem Cells) Fibroblasts Human Umbilical Cord Blood Stem Cell (Hucb)
刊名ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY
2007-02-01
DOI10.1016/j.arthro.2006.09.005
23期:2页:178-187
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Orthopedics ; Surgery
研究领域[WOS]Orthopedics ; Surgery
关键词[WOS]UMBILICAL-CORD BLOOD ; ARTICULAR-CARTILAGE ; PROGENITOR CELLS ; POTENTIAL SOURCE ; STEM-CELLS ; MARROW ; KNEE ; TISSUES ; LESIONS ; BONE
英文摘要

The purpose of this study was to evaluate the repaired tissues formed in full-thickness cartilage defects in a rabbit model implanted with 4 types of chondrogenic cells, including chondrocytes, mesenchymal stem cells (MSCs) and fibroblasts from rabbit, and human umbilical cord blood (hUCB) stem cells. Methods: Chondrocytes, MSCs, and fibroblasts were isolated from 6-week-old New Zealand rabbits; hUCB stem cells were isolated from the umbilical cord blood of newborn children. These 4 types of cells were cultured in vitro and embedded in polylactic acid (PLA) matrices. Full-thickness defects were produced in the femoral trochlear grooves of both knees in 36 adult New Zealand White rabbits. Cell/PLA composites were transplanted into cartilage defects. A total of 5 groups were formed according to implanted cell type: Group A, chondrocytes; Group B, MSCs; Group C, fibroblasts; Group D, hUCB stem cells; and Group E, no cells (control group). Repaired tissues were evaluated grossly, histologically, and immunohistochemically at 6 weeks and 12 weeks after implantation. Results: In Groups A and 13, defects were repaired with hyaline-like cartilage. In Group C, defects were repaired with fibrous tissue. In Group D, defects were repaired primarily with fibrous tissue and scattered chondrocytes; in some specimens, defects were repaired with a thin layer of hyaline-like cartilage at 12 weeks. In Group E, defects were repaired with fibrous tissue. Histologic scores in Groups A and B were significantly higher than those in Groups C, D, and E at 6 and 12 weeks after transplantation. Conclusions: Full-thickness cartilage defects treated with chondrocyte or MSC transplantation were repaired with hyaline-like cartilage tissue, and repair was significantly better than in tissues treated with fibroblasts and hUCB stem cells, as well as in the control group. Repaired tissues treated with MSCs appeared to have better cell arrangement, subchondral bone remodeling, and integration with surrounding cartilage than did repaired tissues generated by chondrocyte implantation. MSCs might be the most suitable cell source for cartilage repair. Further investigation into hUCB stem cell transplantation is needed. Clinical Relevance: In our study of rabbits, MSCs supplied the most promising cell source for cartilage repair.

语种英语
WOS记录号WOS:000244298000012
引用统计
被引频次:115[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51602
专题北京大学第三临床医学院_运动医学研究所
北京大学第一临床医学院_儿科
作者单位Peking Univ, Hosp 3, Inst Sports Med, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Yan, Hui,Yu, Changlong. Repair of full-thickness cartilage defects with cells of different origin in a rabbit model[J]. ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY,2007,23(2):178-187.
APA Yan, Hui,&Yu, Changlong.(2007).Repair of full-thickness cartilage defects with cells of different origin in a rabbit model.ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY,23(2),178-187.
MLA Yan, Hui,et al."Repair of full-thickness cartilage defects with cells of different origin in a rabbit model".ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY 23.2(2007):178-187.
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