IR@PKUHSC  > 北京大学第三临床医学院  > 运动医学研究所
学科主题临床医学
Repair of full-thickness cartilage defects with cells of different origin in a rabbit model
Yan, Hui; Yu, Changlong
关键词Full-thickness Repair Cell Transplantation Chondrocytes Mscs (Mesenchymal Stem Cells) Fibroblasts Human Umbilical Cord Blood Stem Cell (Hucb)
刊名ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY
2007-02-01
DOI10.1016/j.arthro.2006.09.005
23期:2页:178-187
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Orthopedics ; Surgery
研究领域[WOS]Orthopedics ; Surgery
关键词[WOS]UMBILICAL-CORD BLOOD ; ARTICULAR-CARTILAGE ; PROGENITOR CELLS ; POTENTIAL SOURCE ; STEM-CELLS ; MARROW ; KNEE ; TISSUES ; LESIONS ; BONE
英文摘要

The purpose of this study was to evaluate the repaired tissues formed in full-thickness cartilage defects in a rabbit model implanted with 4 types of chondrogenic cells, including chondrocytes, mesenchymal stem cells (MSCs) and fibroblasts from rabbit, and human umbilical cord blood (hUCB) stem cells. Methods: Chondrocytes, MSCs, and fibroblasts were isolated from 6-week-old New Zealand rabbits; hUCB stem cells were isolated from the umbilical cord blood of newborn children. These 4 types of cells were cultured in vitro and embedded in polylactic acid (PLA) matrices. Full-thickness defects were produced in the femoral trochlear grooves of both knees in 36 adult New Zealand White rabbits. Cell/PLA composites were transplanted into cartilage defects. A total of 5 groups were formed according to implanted cell type: Group A, chondrocytes; Group B, MSCs; Group C, fibroblasts; Group D, hUCB stem cells; and Group E, no cells (control group). Repaired tissues were evaluated grossly, histologically, and immunohistochemically at 6 weeks and 12 weeks after implantation. Results: In Groups A and 13, defects were repaired with hyaline-like cartilage. In Group C, defects were repaired with fibrous tissue. In Group D, defects were repaired primarily with fibrous tissue and scattered chondrocytes; in some specimens, defects were repaired with a thin layer of hyaline-like cartilage at 12 weeks. In Group E, defects were repaired with fibrous tissue. Histologic scores in Groups A and B were significantly higher than those in Groups C, D, and E at 6 and 12 weeks after transplantation. Conclusions: Full-thickness cartilage defects treated with chondrocyte or MSC transplantation were repaired with hyaline-like cartilage tissue, and repair was significantly better than in tissues treated with fibroblasts and hUCB stem cells, as well as in the control group. Repaired tissues treated with MSCs appeared to have better cell arrangement, subchondral bone remodeling, and integration with surrounding cartilage than did repaired tissues generated by chondrocyte implantation. MSCs might be the most suitable cell source for cartilage repair. Further investigation into hUCB stem cell transplantation is needed. Clinical Relevance: In our study of rabbits, MSCs supplied the most promising cell source for cartilage repair.

语种英语
WOS记录号WOS:000244298000012
Citation statistics
Cited Times:109[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51602
Collection北京大学第三临床医学院_运动医学研究所
作者单位Peking Univ, Hosp 3, Inst Sports Med, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Yan, Hui,Yu, Changlong. Repair of full-thickness cartilage defects with cells of different origin in a rabbit model[J]. ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY,2007,23(2):178-187.
APA Yan, Hui,&Yu, Changlong.(2007).Repair of full-thickness cartilage defects with cells of different origin in a rabbit model.ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY,23(2),178-187.
MLA Yan, Hui,et al."Repair of full-thickness cartilage defects with cells of different origin in a rabbit model".ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY 23.2(2007):178-187.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Yan, Hui]'s Articles
[Yu, Changlong]'s Articles
百度学术
百度学术Similar articles in
[Yan, Hui]'s Articles
[Yu, Changlong]'s Articles
必应学术
必应学术Similar articles in
[Yan, Hui]'s Articles
[Yu, Changlong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.