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学科主题临床医学
Enhanced sensitivity to mitomycin C by abating heat shock protein 70 expression in human bladder cancer cell line of BIU-87
He, LF1; Guan, KP1; Yan, Z1; Ye, HY1; Xu, KX1; Ren, L1; Hou, SK1
关键词Heat Shock Protein 70 Antisense Oligomers Apoptosis Bladder Cancer Mitomycin c
刊名CHINESE MEDICAL JOURNAL
2005-12-05
118期:23页:1965-1972
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]HEAT-SHOCK-PROTEIN ; ANTISENSE OLIGONUCLEOTIDES ; PROSTATE-CANCER ; BREAST-CANCER ; TUMOR-CELLS ; INHIBITION ; APOPTOSIS ; RESISTANCE ; INDUCTION ; HSP70
英文摘要

Background Bladder cancer is a relatively common tumor in the urinary system, in which mitomycin C (MMC)-based chemotherapy or combination chemotherapy has been mainly used to treat patients with advanced bladder cancer. The prognosis of patients with advanced bladder cancer is still extremely poor in spite of recent therapeutic advances. To improve the prognosis, the sensitivity of tumor cells to mitomycin C by the induction of apoptosis with the abating heat shock protein 70 (HSP70) expression in human bladder cancer cell lines of BIU-87 was investigated.

Methods HSP70 expression was abated in BIU-87 cells by HSP mRNA antisense oligomers. MTT assay and the clone-forming test were used for evaluating the sensitivity of cells to MMC. Apoptosis was assessed using both fluorescent microscopy after staining the cells with Hoechst 33258 and DNA fragment ladder agarose electrophoresis. Thirty-two male six-week-old BALB/c nude mice, at the beginning of the experiment, were used to evaluate the effect of antisense oligomers (ASO) on the tumor formation in vivo.

Results HSP70 expression in BIU-87 was effectively abated by HSP70 mRNA antisense oligomers. The percentage of apoptotic cells in ASO group was greater than in sense oligomers ( SO) [P < 0.05, (18.3 +/- 12.89)% vs (1.89 +/- 0.74)%], nonsense oligomers (NO) [P < 0.05, (18.3 +/- 12.89)% vs (1.78 0.92)%] and blank groups [P < 0.05, ( 18.31 +/- 2.89) % vs ( 1.87 +/- 0.84)%], while the sensitivity of tumor cells to mitomycin C was enhanced. The in vivo tumor inhibition rate of ASO plus MMC ( > 50%) was more than that of ASO or MMC group alone (all P < 0.05).

Conclusions The abating level of HSP70 expression can strengthen the sensitivity of BIU-87 to MMC. One of this effect might be related to the induction of apoptosis by abating HSP70 expression.

语种英语
WOS记录号WOS:000233881700005
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被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51610
专题北京大学第二临床医学院_泌尿外科
北京大学口腔医学院_教育处
作者单位1.Peking Univ, Peoples Hosp, Dept Urol, Beijing 100044, Peoples R China
2.Chinese Acad Med Sci, Peking Union Med Coll, Dept Urol, Canc Hosp & Inst, Beijing 100021, Peoples R China
3.Peking Univ, Peoples Hosp, Cent Lab, Beijing 100044, Peoples R China
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GB/T 7714
He, LF,Guan, KP,Yan, Z,et al. Enhanced sensitivity to mitomycin C by abating heat shock protein 70 expression in human bladder cancer cell line of BIU-87[J]. CHINESE MEDICAL JOURNAL,2005,118(23):1965-1972.
APA He, LF.,Guan, KP.,Yan, Z.,Ye, HY.,Xu, KX.,...&Hou, SK.(2005).Enhanced sensitivity to mitomycin C by abating heat shock protein 70 expression in human bladder cancer cell line of BIU-87.CHINESE MEDICAL JOURNAL,118(23),1965-1972.
MLA He, LF,et al."Enhanced sensitivity to mitomycin C by abating heat shock protein 70 expression in human bladder cancer cell line of BIU-87".CHINESE MEDICAL JOURNAL 118.23(2005):1965-1972.
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