IR@PKUHSC  > 北京大学基础医学院  > 生理学与病理生理学系
学科主题基础医学
Calcitonin gene-related peptide inhibits interleukin-1 beta-induced interleukin-8 secretion in human type II alveolar epithelial cells
Li, Wen-jing; Wang, Teng-ke; Wang, Xian
关键词type II alveolar epithelial cells interleukin-1 interleukin-8 calcitonin gene-related peptide anti-inflammation lung reactive oxygen species
刊名ACTA PHARMACOLOGICA SINICA
2006-10-01
DOI10.1111/j.1745-7254.2006.00408.x
27期:10页:1340-1345
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]EXPRESSION ; RECEPTOR ; ACTIVATION ; CYTOKINES ; CAPSAICIN ; KINASE ; CGRP
英文摘要

Aim: Our previous data have shown that type II alveolar epithelial (AEII) cells express neuropeptide calcitonin gene-related peptide (CGRP), and that pro-inflammatory factor interleukin1-beta (IL-1 beta) induces CGRP secretion in the A549 human AEII cell line. In the present study, we investigated the effect of endogenous and exogenous CGRP on IL-1 beta-induced chemokine interleukin-8 (IL-8) secretion. Methods: We used enzyme-linked immunosorbent assay (ELISA) and RT-PCRto detect IL-8 protein and mRNA levels, respectively. siRNA and the stably trans-fected cell line were used to knock down and overexpress the CGRP gene, respectively, and chemiluminescence assay was used to detect reactive oxygen species (ROS) formation. Results: CGRP-1 receptor antagonist hCGRP(8-37) (0.1-1 nmol-L-1) greatly amplified IL-1 beta-induced IL-8 production. The inhibition of CGRP expression by siRNA significantly increased IL-8 secretion upon IL-1 beta stimulation. However, cell clones stably transfected with CGRP showed significantly inhibited mRNAandproteinlevelsofIL-8 inducedbyIL-1 beta. Conclusion: These data imply that AEII cell-derived CGRP suppress IL-1 beta-induced IL-8 secretion in an autocrine/paracrine mode. Further investigation showed that CGRP attenuated IL-1 beta-aroused ROS formation, which is an early indication of pro-inflammatory factor signaling.

语种英语
WOS记录号WOS:000240685400008
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51613
Collection北京大学基础医学院_生理学与病理生理学系
北京大学基础医学院
作者单位Peking Univ, Sch Basic Med Sci, Dept Physiol, Key Lab Mol Cardiovasc Sci,Minist Educ, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Li, Wen-jing,Wang, Teng-ke,Wang, Xian. Calcitonin gene-related peptide inhibits interleukin-1 beta-induced interleukin-8 secretion in human type II alveolar epithelial cells[J]. ACTA PHARMACOLOGICA SINICA,2006,27(10):1340-1345.
APA Li, Wen-jing,Wang, Teng-ke,&Wang, Xian.(2006).Calcitonin gene-related peptide inhibits interleukin-1 beta-induced interleukin-8 secretion in human type II alveolar epithelial cells.ACTA PHARMACOLOGICA SINICA,27(10),1340-1345.
MLA Li, Wen-jing,et al."Calcitonin gene-related peptide inhibits interleukin-1 beta-induced interleukin-8 secretion in human type II alveolar epithelial cells".ACTA PHARMACOLOGICA SINICA 27.10(2006):1340-1345.
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