IR@PKUHSC  > 北京大学基础医学院  > 药理学系
学科主题基础医学
Down-regulation of brain-pancreas relative protein in diabetic rats and by high glucose in PC12 cells: Prevention by calpain inhibitors
Tie, Lu1,2; Xu, Ying1,2; Lin, Yan-Hua1,2; Yao, Xiao-Hao1,2; Wu, Hong-Li1,2; Lit, Yu-Hua1,2; Shen, Zhu-Fang3,4; Yu, He-Ming5; Li, Xue-Jun1,2
关键词Brain-pancreas Relative Protein Diabetes High Glucose Proteasome Inhibitor Calpeptin
刊名JOURNAL OF PHARMACOLOGICAL SCIENCES
2008
DOI10.1254/jphs.FP0071092
106期:1页:28-37
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]PROTEOLYTIC SYSTEMS ; MELLITUS ; INSULIN ; DYSFUNCTION ; COMPLICATIONS ; DEGRADATION ; MECHANISM ; ISCHEMIA ; CALCIUM ; INJURY
英文摘要

Brain-pancreas relative protein (BPRP) is a novel protein that we found in our laboratory. Previously we demonstrated that it is involved in ischemia and depression. In light of the putative association between diabetes and clinical depression, and the selective expression of BPRP in brain and pancreas, the present study examined whether BPRP levels are affected by induction of diabetes by alloxan injection in rats and exposure to high glucose levels in PC12 cells. Western blot and immunohistochemical analyses revealed that BPRP levels were decreased in the hippocampal CA1 neurons of diabetic rats 4 and 8 weeks post-alloxan injection and in PC12 cells 48 h after exposure to high concentrations of glucose. BPRP protein levels were not affected by osmolarity control treatments with mannitol. Follow-up pharmacological experiments in PC12 cells revealed that glucose-induced BPRP down-regulation was markedly attenuated by the calpain inhibitors N-acetyl-Leu-Leu-norleucinal (ALLN) or calpeptin, but not the proteasome-specific inhibitor carbobenzoxy-Leu-Leu-leucinal (MG132). The ability of calpain inhibitors to specifically counter the effects of high glucose exposure on BPRP levels further suggests that BPRP and calpain activity may contribute to diabetes complications in the central nervous system.

语种英语
WOS记录号WOS:000252819900006
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51618
Collection北京大学基础医学院_药理学系
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Pharmacol, Beijing 100083, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
3.Peking Union Med Coll, Beijing 100050, Peoples R China
4.Chinese Acad Med Sci, Inst Mat Med, Beijing 100050, Peoples R China
5.Nat Res Inst Family Planning, Beijing 100081, Peoples R China
Recommended Citation
GB/T 7714
Tie, Lu,Xu, Ying,Lin, Yan-Hua,et al. Down-regulation of brain-pancreas relative protein in diabetic rats and by high glucose in PC12 cells: Prevention by calpain inhibitors[J]. JOURNAL OF PHARMACOLOGICAL SCIENCES,2008,106(1):28-37.
APA Tie, Lu.,Xu, Ying.,Lin, Yan-Hua.,Yao, Xiao-Hao.,Wu, Hong-Li.,...&Li, Xue-Jun.(2008).Down-regulation of brain-pancreas relative protein in diabetic rats and by high glucose in PC12 cells: Prevention by calpain inhibitors.JOURNAL OF PHARMACOLOGICAL SCIENCES,106(1),28-37.
MLA Tie, Lu,et al."Down-regulation of brain-pancreas relative protein in diabetic rats and by high glucose in PC12 cells: Prevention by calpain inhibitors".JOURNAL OF PHARMACOLOGICAL SCIENCES 106.1(2008):28-37.
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