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IR@PKUHSC  > 北京大学临床肿瘤学院  > 胃肠肿瘤中心  > 期刊论文
学科主题: 临床医学
题名:
High-level SAE2 promotes malignant phenotype and predicts outcome in gastric cancer
作者: Shao, Duan-Fang1; Wang, Xiao-Hong4; Li, Zi-Yu1; Xing, Xiao-Fang2; Cheng, Xiao-Jing2; Guo, Ting2; Du, Hong2; Hu, Ying4; Dong, Bin3; Ding, Ning1; Li, Lin1; Li, Shen1; Li, Qing-Da1; Wen, Xian-Zi2; Zhang, Lian-Hai1; Ji, Jia-Fu1
关键词: SAE2 ; gastric cancer ; prognosis ; FoxM1 ; c-MYC
刊名: AMERICAN JOURNAL OF CANCER RESEARCH
发表日期: 2015
卷: 5, 期:1, 页:140-154
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: CELL-CYCLE ; SUMOYLATION ; SUMO ; PROGRESSION ; METASTASIS ; TRANSCRIPTION ; EXPRESSION ; SURVIVAL ; INVASION ; PROTEIN
英文摘要:

Background: The SUMO pathway has been shown to play an important role in tumorigenesis. This report analyzed the involvement of the sole SUMO-Activating Enzyme Subunit 2 (SAE2) in human gastric cancer (GC) progression and prognosis. Methods: Expression of SAE2 was examined by Quantigene Plex, western blotting and immunohistochemistry. The expression of SAE2 and c-MYC were detected in parallel in 276 cases. The molecular mechanisms of SAE2 expression and its effects on cell growth, colony formation, migration and invasion were also explored by CCK8 assay, colony formation experiment, transwell chamber assay with or without matrigel, immunoprecipitation and in vivo tumorigenesis and tumor metastasis. Results: SAE2 was markedly overexpressed in GC cell lines and primary tumor samples of GC, and significantly correlated with deeper tumor depth, distant metastasis, higher pathological stage and stratified survival in human GC. SAE2 positivity was independently associated with a worse outcome in multivariate analysis. Knockdown of SAE2 expression inhibited the proliferation, migration, and invasion of SAE2-overexpressing GC cells. Consistent with the in vitro results, down-regulation of SAE2 in human GC BGC823 cells significantly reduced the tumorigenic and metastatic potential of the cells in vivo. SAE2 protein was significantly associated with the higher expression of c-MYC in primary GC tissues. Moreover, FoxM1 was SUMOylated in GC and that inhibition of SAE2 resulted in a decrease in SUMO1-FoxM1 levels compared with those in the controls. Conclusions: These findings suggest that SAE2 has a pivotal role in the aggressiveness of GC, and highlight its usefulness as a prognostic factor in GC.

语种: 英语
所属项目编号: 81101879 ; 2012-AA02A203-B01 ; 2012AA02A504-B01 ; 2012-AA020101 ; Z121100007512-010 ; D131100005313010
项目资助者: National Natural Science Foundation of China ; National Key Technology RD Program ; Beijing Municipal Science &amp ; Technology Commission
WOS记录号: WOS:000348300400010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51625
Appears in Collections:北京大学临床肿瘤学院_胃肠肿瘤中心_期刊论文

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作者单位: 1.Peking Univ, Dept Gastrointestinal Surg, Key Lab Carcinogenesis & Translat Res, Minist Educ,Beijing Canc Hosp & Inst,Sch Oncol, Beijing 100871, Peoples R China
2.Peking Univ, Dept Gastrointestinal Translat Res, Key Lab Carcinogenesis & Translat Res, Minist Educ,Beijing Canc Hosp & Inst,Sch Oncol, Beijing 100871, Peoples R China
3.Peking Univ, Dept Pathol, Key Lab Carcinogenesis & Translat Res, Minist Educ,Beijing Canc Hosp & Inst,Sch Oncol, Beijing 100871, Peoples R China
4.Peking Univ, Tissue Bank, Key Lab Carcinogenesis & Translat Res, Minist Educ,Beijing Canc Hosp & Inst,Sch Oncol, Beijing 100871, Peoples R China

Recommended Citation:
Shao, Duan-Fang,Wang, Xiao-Hong,Li, Zi-Yu,et al. High-level SAE2 promotes malignant phenotype and predicts outcome in gastric cancer[J]. AMERICAN JOURNAL OF CANCER RESEARCH,2015,5(1):140-154.
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