学科主题临床医学
Mitochondrially localized EGFR is independent of its endocytosis and associates with cell viability
Yao, Yuan2; Wang, Gang3,4,5; Li, Zhe6; Yan, Bing3,4,5; Guo, Yinglu3,4,5; Jiang, Xuejun1; Xi, Zhijun3,4,5
关键词Epidermal Growth Factor Receptor Endocytosis Mitochondria Gefitinib Cell Viability
刊名ACTA BIOCHIMICA ET BIOPHYSICA SINICA
2010-11-01
DOI10.1093/abbs/gmq090
42期:11页:763-770
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]GROWTH-FACTOR RECEPTOR ; ELECTRON-MICROSCOPY ; TYROSINE KINASES ; CYTOCHROME-C ; CANCER ; PHOSPHORYLATION ; INTERNALIZATION ; APOPTOSIS ; PATHWAYS ; SURVIVAL
英文摘要

The molecular mechanism underlying epidermal growth factor receptor (EGFR) localization in mitochondria remains largely unknown. Using immune electron microscopy, we validated that EGFR could be localized on either the outer or the inner membrane of mitochondria. Mutant receptor lacked amino acids 646-660 was flawed in migration onto the organelles, whereas the mutated receptor with a defective endocytosis showed a greater capability of moving onto mitochondria upon stimulation of epidermal growth factor (EGF). Gefitinib, an inhibitor of EGFR kinase, inhibited the receptor endocytosis after short time of treatment, yet, only reduced cell viability as well as the amount of mitochondrial EGFR after longer time of exposure. Moreover, the content of mitochondrial EGFR transfer was decreased when the cells were exposed to the apoptotic inducer etoposide. EGF-induced programmed cell death usually coincided with a decline in mitochondrial EGFR. These data indicated that the mitochondrial-localized EGFR is independent of its internalization and may be correlated with cell survival and participate in the ligand-induced programmed cell death.

语种英语
WOS记录号WOS:000283657600001
项目编号90408024 ; 30470855 ; 30571853 ; 30872588
资助机构National Natural Science Foundation of China
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51627
专题北京大学第一临床医学院_北京大学泌尿外科研究所
北京大学第一临床医学院_泌尿外科
北京大学第一临床医学院_胸外科
作者单位1.Peking Univ, Yuanpei Sch, Beijing 100871, Peoples R China
2.Natl Res Ctr Urol Canc, Beijing 100034, Peoples R China
3.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China
4.Peking Univ, Inst Urol, Beijing 100034, Peoples R China
5.Chinese Acad Sci, Inst Microbiol, Biol Resource Ctr, Beijing 100101, Peoples R China
6.Peking Univ, Beijing JiShuiTan Hosp, Clin Med Coll 4, Beijing 100035, Peoples R China
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GB/T 7714
Yao, Yuan,Wang, Gang,Li, Zhe,et al. Mitochondrially localized EGFR is independent of its endocytosis and associates with cell viability[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2010,42(11):763-770.
APA Yao, Yuan.,Wang, Gang.,Li, Zhe.,Yan, Bing.,Guo, Yinglu.,...&Xi, Zhijun.(2010).Mitochondrially localized EGFR is independent of its endocytosis and associates with cell viability.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,42(11),763-770.
MLA Yao, Yuan,et al."Mitochondrially localized EGFR is independent of its endocytosis and associates with cell viability".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 42.11(2010):763-770.
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