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学科主题基础医学
Analysis of Hepatitis B Virus Intrahepatic Covalently Closed Circular DNA and Serum Viral Markers in Treatment-Naive Patients with Acute and Chronic HBV Infection
Li, Weijie1,2,3; Zhao, Jingmin4; Zou, Zhengsheng2,3; Liu, Yan2,3; Li, Baosen2,3; Sun, Ying2,3; Li, Xiaodong2,3; Liu, Shuhong4; Cai, Shaoping2,3; Yao, Weimin2,3; Xin, Shaojie2,3; Lu, Fengmin1; Xu, Dongping1,2,3
刊名PLOS ONE
2014-02-13
DOI10.1371/journal.pone.0089046
9期:2
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Natural Science Foundation of China ; 12th Five-Year High-Tech Grand ; 12th Five-Year Special Grand project for Infectious Diseases ; National Natural Science Foundation of China ; 12th Five-Year High-Tech Grand ; 12th Five-Year Special Grand project for Infectious Diseases
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]SURFACE-ANTIGEN ; NATURAL-HISTORY ; REPLICATIVE ACTIVITY ; LIVER-TISSUE ; CCCDNA ; ADEFOVIR ; QUANTITATION ; THERAPY ; PERSISTENCE ; REDUCTION
英文摘要

Background: This study aimed to investigate the relationships of intrahepatic cccDNA with serum HBsAg and with HBV DNA in treatment-naive patients throughout acute and chronic HBV infection.

Methods: A total of 120 patients who had a liver biopsy were enrolled, including 19 with acute hepatitis B (AHB), and 101 patients with chronic HBV infection (CHB) of whom were 10 in immune-tolerant (IT) phase, 59 in immune-clearance (IC) phase, 8 in low-replicative (LR) phase, and 24 in HBeAg-negative hepatitis (ENH) phase. Intrahepatic cccDNA, serum HBsAg and serum HBV DNA levels were comparatively analyzed.

Results: The median intrahepatic cccDNA levels were 0.18 4.80, 3.81, 0.22 and 0.97 copies/cell for patients with AHB, CHB-IT, CHB-IC, CHB-LR, and CHB-ENH, respectively. In AHB patients, intrahepatic cccDNA was positively correlated with serum HBsAg (r = 0.665, P = 0.003), as well as serum HBV DNA (r = 0.536, P = 0.022). In CHB patients, intrahepatic cccDNA was positively correlated with serum HBsAg in the IC phase (r = 0.392, P = 0.005), and with serum HBV DNA in the IC phase (r = 0.301, P = 0.036) and ENH phase (r = 0.588, P = 0.013). HBV replicative efficiency, defined as the ratio of serum HBV DNA to intrahepatic cccDNA, was obviously lower in AHB and CHB-LR patients than in CHB-IT, CHB-IC and CHB-ENH patients (0.70 and 0.53 vs. 1.12, 1.09 and 0.99, P<0.001, values were logarithmic transformed for analysis). In CHB-IC patients, HBV replicative efficiency was positively correlated with histological activity index of liver inflammation (r = 0.308, P = 0.009).

Conclusion: Serum HBsAg and HBV DNA levels may reflect the amount of active intrahepatic cccDNA in treatment-naive AHB and CHB-IC patients. Reduced intrahepatic cccDNA and HBV replicative efficiency may imply effective immune control of HBV infection.

语种英语
所属项目编号81171617 ; 2010gxjs009 ; 2012ZX10004503
资助者National Natural Science Foundation of China ; 12th Five-Year High-Tech Grand ; 12th Five-Year Special Grand project for Infectious Diseases ; National Natural Science Foundation of China ; 12th Five-Year High-Tech Grand ; 12th Five-Year Special Grand project for Infectious Diseases
WOS记录号WOS:000331266000094
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51631
专题基础医学院_病原生物学系
作者单位1.Beijing 302 Hosp, Inst Infect Dis, Beijing, Peoples R China
2.Beijing 302 Hosp, Med Ctr Liver Failure, Beijing, Peoples R China
3.Beijing 302 Hosp, Dept Pathol, Beijing, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Li, Weijie,Zhao, Jingmin,Zou, Zhengsheng,et al. Analysis of Hepatitis B Virus Intrahepatic Covalently Closed Circular DNA and Serum Viral Markers in Treatment-Naive Patients with Acute and Chronic HBV Infection[J]. PLOS ONE,2014,9(2).
APA Li, Weijie.,Zhao, Jingmin.,Zou, Zhengsheng.,Liu, Yan.,Li, Baosen.,...&Xu, Dongping.(2014).Analysis of Hepatitis B Virus Intrahepatic Covalently Closed Circular DNA and Serum Viral Markers in Treatment-Naive Patients with Acute and Chronic HBV Infection.PLOS ONE,9(2).
MLA Li, Weijie,et al."Analysis of Hepatitis B Virus Intrahepatic Covalently Closed Circular DNA and Serum Viral Markers in Treatment-Naive Patients with Acute and Chronic HBV Infection".PLOS ONE 9.2(2014).
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