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学科主题: 公共卫生
题名:
All-trans retinoic acid regulates c-jun expression via ERK5 in cardiac myoblasts
作者: Ren, Xia1; Ma, Xi2; Li, Yong1
关键词: all-trans retinoic acid ; ERK5 ; ERK1 ; c-jun ; translocation ; proliferation
刊名: JOURNAL OF NUTRITIONAL BIOCHEMISTRY
发表日期: 2007-12-01
DOI: 10.1016/j.jnutbio.2006.12.023
卷: 18, 期:12, 页:832-838
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Nutrition & Dietetics
研究领域[WOS]: Biochemistry & Molecular Biology ; Nutrition & Dietetics
关键词[WOS]: SIGNAL-TRANSDUCTION ; MAP KINASE ; CELL-PROLIFERATION ; TRANSCRIPTION FACTOR ; INDUCED APOPTOSIS ; GENE-EXPRESSION ; PATHWAY ; BMK1/ERK5 ; BINDING ; BMK1
英文摘要:

Retinoic acid (RA) is an active metabolite of vitamin A and plays an important role in biological processes including cell proliferation. MAPKs play a pivotal role in regulating many critical cellular processes in the heart. The aim of the study was to determine whether all-trans RA (atRA) affects the proliferation of H9c2 rat ventricular cells and whether ERK family is involved in this process. H9c2 myocardial cells were cultured and subjected to MTT and H-3-thymidine incorporation assays for proliferation detection. Luciferase reporter gene and Western blot assays were used to detect the transcription and protein expression of c-jun. In addition, the activities of EPK5 and ERK1 were determined by Western blot assay. The subcellular distribution of ERK5 and ERK1 was analyzed by confocal microscopy. It was shown that atRA (0.05 mu M) facilitated the proliferation of H9c2 myocardial cells and increased the transcription and protein expression of c-jun. Inhibition of ERK5 significantly decreased atRA-induced pJluc expression (P <.01). The activity of ERK5 but not ERK1 was induced by atRA. Furthermore, atRA promoted the nuclear translocation of ERK5 but not ERK1. These results suggest that ERK5 pathway may be involved in the process that atRA regulates proliferation in the developing heart. (c) 2007 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000251701800009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51635
Appears in Collections:北京大学公共卫生学院_期刊论文

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作者单位: 1.Peking Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Lab Dev Mol Biol,Hlth Sci Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Hlth Sci Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Ren, Xia,Ma, Xi,Li, Yong. All-trans retinoic acid regulates c-jun expression via ERK5 in cardiac myoblasts[J]. JOURNAL OF NUTRITIONAL BIOCHEMISTRY,2007,18(12):832-838.
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