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学科主题: 药学
题名:
Simultaneous determination of triterpene saponins in Ginseng drugs by high-performance liquid chromatography
作者: Zhu, S1; Zou, K1; Cai, SQ1; Meselhy, MR1; Komatsu, K1
关键词: ginsenoside ; HPLC method ; Ginseng drug ; Panax spp. ; quantitative comparison
刊名: CHEMICAL & PHARMACEUTICAL BULLETIN
发表日期: 2004-08-01
卷: 52, 期:8, 页:995-998
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Medicinal ; Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy ; Chemistry
关键词[WOS]: PANACIS-JAPONICI RHIZOMA ; N-SH CELLS ; GINSENOSIDES ; CONSTITUENTS ; PLANTS ; RADIX
英文摘要:

A HPLC method for the simultaneous determination of 11 triterpene saponins with four-type aglycones (protopanaxadiol, protopanaxatriol, ocotillol and oleanolic acid types) in Ginseng drugs was developed and validated. Using a gradient of acetonitrile and 10 mm K-phosphate buffer (pH 5.80) as the mobile phase and UV detection at 196 nm, more than 18 ginsenosides with different aglycones were separated satisfactorily within 60 min. The detection limits (signal/noise greater than or equal to3) were 0.1 mug for ginsenosides Rb-1, Rc, Rd, Re and Rg(1), chikusetsusaponin III, and notoginsenoside R-2, 0.2 mug for gisenoside R-0 and chikusetsusaponin IVa, 0.3 mug for chikusetsusaponin IV, and 3 mug for majonoside R-2. The calibration curve of each saponin had a correlation coefficient close to 1. Intraand interday precisions were less than 2.1% (n=5) and 3.3% (n=15), respectively. The recovery rates of extraction were in the range of 96.4-102.7% for all ginsenosides. By adopting this method, the determinations of 11 ginsenosides in three Ginseng drugs derived from Panax ginseng, Panax vietnamensis var. fuscidiscus and Panax japonicus (Japan) were achieved.

语种: 英语
WOS记录号: WOS:000223208200021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51641
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Gorges Univ 3, Chem & Life Sci Coll, Hubei 443003, Peoples R China
2.Toyama Med & Pharmaceut Univ, Inst Nat Med, Res Ctr Ethnomed, Toyama 9300194, Japan
3.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

Recommended Citation:
Zhu, S,Zou, K,Cai, SQ,et al. Simultaneous determination of triterpene saponins in Ginseng drugs by high-performance liquid chromatography[J]. CHEMICAL & PHARMACEUTICAL BULLETIN,2004,52(8):995-998.
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