IR@PKUHSC  > 北京大学药学院
学科主题药学
Simultaneous determination of triterpene saponins in Ginseng drugs by high-performance liquid chromatography
Zhu, S1; Zou, K1; Cai, SQ1; Meselhy, MR1; Komatsu, K1
关键词Ginsenoside Hplc Method Ginseng Drug Panax Spp. Quantitative Comparison
刊名CHEMICAL & PHARMACEUTICAL BULLETIN
2004-08-01
52期:8页:995-998
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Medicinal ; Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy ; Chemistry
关键词[WOS]PANACIS-JAPONICI RHIZOMA ; N-SH CELLS ; GINSENOSIDES ; CONSTITUENTS ; PLANTS ; RADIX
英文摘要

A HPLC method for the simultaneous determination of 11 triterpene saponins with four-type aglycones (protopanaxadiol, protopanaxatriol, ocotillol and oleanolic acid types) in Ginseng drugs was developed and validated. Using a gradient of acetonitrile and 10 mm K-phosphate buffer (pH 5.80) as the mobile phase and UV detection at 196 nm, more than 18 ginsenosides with different aglycones were separated satisfactorily within 60 min. The detection limits (signal/noise greater than or equal to3) were 0.1 mug for ginsenosides Rb-1, Rc, Rd, Re and Rg(1), chikusetsusaponin III, and notoginsenoside R-2, 0.2 mug for gisenoside R-0 and chikusetsusaponin IVa, 0.3 mug for chikusetsusaponin IV, and 3 mug for majonoside R-2. The calibration curve of each saponin had a correlation coefficient close to 1. Intraand interday precisions were less than 2.1% (n=5) and 3.3% (n=15), respectively. The recovery rates of extraction were in the range of 96.4-102.7% for all ginsenosides. By adopting this method, the determinations of 11 ginsenosides in three Ginseng drugs derived from Panax ginseng, Panax vietnamensis var. fuscidiscus and Panax japonicus (Japan) were achieved.

语种英语
WOS记录号WOS:000223208200021
引用统计
被引频次:35[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51641
专题北京大学药学院
作者单位1.Gorges Univ 3, Chem & Life Sci Coll, Hubei 443003, Peoples R China
2.Toyama Med & Pharmaceut Univ, Inst Nat Med, Res Ctr Ethnomed, Toyama 9300194, Japan
3.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Zhu, S,Zou, K,Cai, SQ,et al. Simultaneous determination of triterpene saponins in Ginseng drugs by high-performance liquid chromatography[J]. CHEMICAL & PHARMACEUTICAL BULLETIN,2004,52(8):995-998.
APA Zhu, S,Zou, K,Cai, SQ,Meselhy, MR,&Komatsu, K.(2004).Simultaneous determination of triterpene saponins in Ginseng drugs by high-performance liquid chromatography.CHEMICAL & PHARMACEUTICAL BULLETIN,52(8),995-998.
MLA Zhu, S,et al."Simultaneous determination of triterpene saponins in Ginseng drugs by high-performance liquid chromatography".CHEMICAL & PHARMACEUTICAL BULLETIN 52.8(2004):995-998.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhu, S]的文章
[Zou, K]的文章
[Cai, SQ]的文章
百度学术
百度学术中相似的文章
[Zhu, S]的文章
[Zou, K]的文章
[Cai, SQ]的文章
必应学术
必应学术中相似的文章
[Zhu, S]的文章
[Zou, K]的文章
[Cai, SQ]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。