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Surface modification on polycaprolactone electrospun mesh and human decalcified bone scaffold with synovium-derived mesenchymal stem cells-affinity peptide for tissue engineering
Shao, Zhenxing1; Zhang, Xin1; Pi, Yanbin1; Yin, Ling2; Li, La1; Chen, Haifeng2; Zhou, Chunyan3; Ao, Yingfang1
关键词Synovium-derived Mesenchymal Stem Cell Phage Display Affinity Peptide Tissue Engineering Surface Modification
刊名JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
2015
DOI10.1002/jbm.a.35177
103期:1页:318-329
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]REPAIR OSTEOCHONDRAL DEFECTS ; THERAPY POSITION STATEMENT ; CELLULAR-THERAPY ; FACTOR-I ; INTERNATIONAL-SOCIETY ; CARTILAGE DEFECTS ; STROMAL CELLS ; RABBIT MODEL ; REGENERATION ; BIOMATERIALS
英文摘要

Synovium-derived mesenchymal stem cells (SMSC) have been studied for over a decade since first being successfully isolated in 2001. These cells demonstrate the most promising therapeutic efficacy for musculoskeletal regeneration of the MSC family, particularly for cartilage regeneration. However, the mobilization and transfer of MSCs to defective or damaged tissues and organs in vivo with high accuracy and efficiency has been a major problem in tissue engineering (TE). In the present study, we identified a seven amino acid peptide sequence [SMSCs-affinity peptide (LTHPRWP; L7)] through phage display technology that has a high specific affinity to SMSCs. Our analysis suggested that L7 efficiently and specifically interacted with SMSCs without any species specificity. Thereafter, L7 was covalently conjugated onto both polycaprolactone (PCL) electrospun meshes and human decalcified bone scaffolds (hDBSc) to investigate its TE applications. After 24 h coculture with human SMSCs (hSMSCs), L7-conjugated PCL electrospun meshes had significantly more adherent hSMSCs than the control group, and the cells expanded well. Similar results were obtained using hDBSs. These results suggest that the novel L7 peptide sequence has a high specific affinity to SMSCs. Covalently conjugating this peptide to either artificial polymer material (PCL mesh) or natural material (hDBS) significantly enhances the adhesion of SMSCs. This method is applicable to a wide range of potential SMSC-based TE applications, particularly to cartilage regeneration, via surface modification on various type of materials. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 318-329, 2015.

语种英语
WOS记录号WOS:000345572100036
项目编号81071474 ; 81171726 ; 20110001130001 ; 2012CB933903
资助机构National Natural Science Foundation of China (NSFC) ; Specialized Research Fund for the Doctoral Program of Higher Education ; National Basic Research Program of China
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51642
专题北京大学第三临床医学院_运动医学研究所
北京大学基础医学院
北京大学第一临床医学院_妇产科
北京大学第三临床医学院_骨科
作者单位1.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100191, Peoples R China
2.Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
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GB/T 7714
Shao, Zhenxing,Zhang, Xin,Pi, Yanbin,et al. Surface modification on polycaprolactone electrospun mesh and human decalcified bone scaffold with synovium-derived mesenchymal stem cells-affinity peptide for tissue engineering[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2015,103(1):318-329.
APA Shao, Zhenxing.,Zhang, Xin.,Pi, Yanbin.,Yin, Ling.,Li, La.,...&Ao, Yingfang.(2015).Surface modification on polycaprolactone electrospun mesh and human decalcified bone scaffold with synovium-derived mesenchymal stem cells-affinity peptide for tissue engineering.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,103(1),318-329.
MLA Shao, Zhenxing,et al."Surface modification on polycaprolactone electrospun mesh and human decalcified bone scaffold with synovium-derived mesenchymal stem cells-affinity peptide for tissue engineering".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 103.1(2015):318-329.
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