北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 运动医学研究所  > 期刊论文
学科主题: 临床医学
题名:
Surface modification on polycaprolactone electrospun mesh and human decalcified bone scaffold with synovium-derived mesenchymal stem cells-affinity peptide for tissue engineering
作者: Shao, Zhenxing1; Zhang, Xin1; Pi, Yanbin1; Yin, Ling2; Li, La1; Chen, Haifeng2; Zhou, Chunyan3; Ao, Yingfang1
关键词: synovium-derived mesenchymal stem cell ; phage display ; affinity peptide ; tissue engineering ; surface modification
刊名: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
发表日期: 2015
DOI: 10.1002/jbm.a.35177
卷: 103, 期:1, 页:318-329
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: REPAIR OSTEOCHONDRAL DEFECTS ; THERAPY POSITION STATEMENT ; CELLULAR-THERAPY ; FACTOR-I ; INTERNATIONAL-SOCIETY ; CARTILAGE DEFECTS ; STROMAL CELLS ; RABBIT MODEL ; REGENERATION ; BIOMATERIALS
英文摘要:

Synovium-derived mesenchymal stem cells (SMSC) have been studied for over a decade since first being successfully isolated in 2001. These cells demonstrate the most promising therapeutic efficacy for musculoskeletal regeneration of the MSC family, particularly for cartilage regeneration. However, the mobilization and transfer of MSCs to defective or damaged tissues and organs in vivo with high accuracy and efficiency has been a major problem in tissue engineering (TE). In the present study, we identified a seven amino acid peptide sequence [SMSCs-affinity peptide (LTHPRWP; L7)] through phage display technology that has a high specific affinity to SMSCs. Our analysis suggested that L7 efficiently and specifically interacted with SMSCs without any species specificity. Thereafter, L7 was covalently conjugated onto both polycaprolactone (PCL) electrospun meshes and human decalcified bone scaffolds (hDBSc) to investigate its TE applications. After 24 h coculture with human SMSCs (hSMSCs), L7-conjugated PCL electrospun meshes had significantly more adherent hSMSCs than the control group, and the cells expanded well. Similar results were obtained using hDBSs. These results suggest that the novel L7 peptide sequence has a high specific affinity to SMSCs. Covalently conjugating this peptide to either artificial polymer material (PCL mesh) or natural material (hDBS) significantly enhances the adhesion of SMSCs. This method is applicable to a wide range of potential SMSC-based TE applications, particularly to cartilage regeneration, via surface modification on various type of materials. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 318-329, 2015.

语种: 英语
所属项目编号: 81071474 ; 81171726 ; 20110001130001 ; 2012CB933903
项目资助者: National Natural Science Foundation of China (NSFC) ; Specialized Research Fund for the Doctoral Program of Higher Education ; National Basic Research Program of China
WOS记录号: WOS:000345572100036
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51642
Appears in Collections:北京大学第三临床医学院_运动医学研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100191, Peoples R China
2.Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China

Recommended Citation:
Shao, Zhenxing,Zhang, Xin,Pi, Yanbin,et al. Surface modification on polycaprolactone electrospun mesh and human decalcified bone scaffold with synovium-derived mesenchymal stem cells-affinity peptide for tissue engineering[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2015,103(1):318-329.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Shao, Zhenxing]'s Articles
[Zhang, Xin]'s Articles
[Pi, Yanbin]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Shao, Zhenxing]‘s Articles
[Zhang, Xin]‘s Articles
[Pi, Yanbin]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  北京大学医学部 - Feedback
Powered by CSpace