IR@PKUHSC  > 北京大学第三临床医学院  > 消化科
学科主题临床医学
Mast cell activation is involved in stress-induced epithelial barrier dysfunction in the esophagus
Zhong, Chan Juan1; Wang, Kun1; Zhang, Lu1; Yang, Chang Qing1; Zhang, Kuo2; Zhou, Shu Pei2; Duan, Li Ping1
关键词Dilated Intercellular Space Mast Cell Stress Tight Junction Tryptase
刊名JOURNAL OF DIGESTIVE DISEASES
2015-04-01
DOI10.1111/1751-2980.12226
16期:4页:186-196
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
资助者National Natural Science Fund ; 12th Five-Year Plan for National Science &amp ; Technology Support Program ; National Natural Science Fund ; 12th Five-Year Plan for National Science &amp ; Technology Support Program
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]CORTICOTROPIN-RELEASING-FACTOR ; DILATED INTERCELLULAR SPACES ; INDUCED VISCERAL HYPERSENSITIVITY ; GASTROESOPHAGEAL-REFLUX DISEASE ; PARACELLULAR PERMEABILITY ; MUCOSAL INFLAMMATION ; RAT ; EXPRESSION ; RECEPTOR-2 ; TRYPTASE
英文摘要

ObjectiveWe aimed to investigate the role of mast cell in stress-induced barrier dysfunction in the esophagus and its possible pathway involved using mast cell-deficient (Ws/Ws) rats.

MethodsWs/Ws rats and normal (+/+) rats were submitted to chronic restraint stress (CRS) 2h/day for 7 days. Tissues were obtained from distal esophagus. Mast cells were counted under Alcian blue-safranin O stain. Activation of mast cells was assessed using transmission electron microscope. Esophageal epithelial barrier dysfunction was evaluated by measuring intercellular spaces (ICS) and by quantifying tight junction (TJ) proteins. The localization and expression of mast cell-derived tryptase and proteinase activated receptor 2 (PAR-2) were assessed.

ResultsA higher number of mast cells and higher proportion of activated mast cells were observed in CRS +/+ rats compared with non-stress controls. Increased ICS and decreased expression of some TJ proteins were observed in the CRS +/+ rats but not in the CRS Ws/Ws rats. Tryptase and its receptor PAR-2 were found elevated concomitantly by nearly 100% in CRS +/+ rats, but not in CRS Ws/Ws rats.

ConclusionsMast cells play an important role in stress-induced epithelial barrier dysfunction in esophagus. The mechanism may involve the activation of PAR-2 by mast cell-derived tryptase, causing proinflammatory responses and the subsequent disruption of the epithelial TJ proteins and a disturbed cytoskeleton function, resulting in dilated intercellular spaces.

语种英语
所属项目编号30940033 ; 2012BAI06B02
资助者National Natural Science Fund ; 12th Five-Year Plan for National Science &amp ; Technology Support Program ; National Natural Science Fund ; 12th Five-Year Plan for National Science &amp ; Technology Support Program
WOS记录号WOS:000354021800003
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51656
Collection北京大学第三临床医学院_消化科
作者单位1.Peking Univ, Dept Gastroenterol, Hosp 3, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Lab Anim Sci, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Zhong, Chan Juan,Wang, Kun,Zhang, Lu,et al. Mast cell activation is involved in stress-induced epithelial barrier dysfunction in the esophagus[J]. JOURNAL OF DIGESTIVE DISEASES,2015,16(4):186-196.
APA Zhong, Chan Juan.,Wang, Kun.,Zhang, Lu.,Yang, Chang Qing.,Zhang, Kuo.,...&Duan, Li Ping.(2015).Mast cell activation is involved in stress-induced epithelial barrier dysfunction in the esophagus.JOURNAL OF DIGESTIVE DISEASES,16(4),186-196.
MLA Zhong, Chan Juan,et al."Mast cell activation is involved in stress-induced epithelial barrier dysfunction in the esophagus".JOURNAL OF DIGESTIVE DISEASES 16.4(2015):186-196.
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