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学科主题: 临床医学
题名:
Analysis of urinary methylated nucleosides of patients with coronary artery disease by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry
作者: Li, Yanru1; Yu, Haiyi2,3,4; Zhao, Wei2,3,4; Xu, Xinye2,3,4; Zhou, Jiang1; Xu, Ming2,3,4; Gao, Wei2,3,4; Yuan, Gu1
刊名: RAPID COMMUNICATIONS IN MASS SPECTROMETRY
发表日期: 2014-10-15
DOI: 10.1002/rcm.6986
卷: 28, 期:19, 页:2054-2058
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical ; Spectroscopy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
关键词[WOS]: POTENTIAL TUMOR-MARKERS ; CANCER-PATIENTS ; BREAST-CANCER ; HEART-FAILURE ; CLINICAL-DIAGNOSIS ; COLORECTAL-CANCER ; MS ANALYSIS ; IDENTIFICATION ; ANGIOTENSIN ; PHENOTYPE
英文摘要:

RATIONALE: In recent years, methylated nucleosides have been considered to be potential biomarkers to human diseases. The early diagnosis of coronary artery disease (CAD) is an unsolved problem in clinical cardiology. The aim of our study is to evaluate whether urinary methylated nucleosides can serve as useful biomarkers for CAD.

METHODS: A solid-phase extraction (SPE) column was used for extraction and purification of methylated nucleosides in urine, and high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC/ESI-MS/MS) was employed for specific, sensitive and rapid determination of the urinary methylated nucleosides from patients with cardiac events.

RESULTS: We have analyzed six methylated nucleosides (N-3-methylcytidine, N-1-methyladenosine, N-6-methyladenosine, N-2-methylguanosine, N-1-methylguanosine and N-2, N-2-dimethylguanosine) in urine from 51 patients with CAD and 25 non-CADcontrols byHPLC/ESI-MS/MS using selective reactionmonitoring (SRM). Our results have shown that there were significant differences in the N-6-methyladenosine levels from the patients and the non-CAD controls in the urine analyzed.

CONCLUSIONS: The results have indicated that HPLC/ESI-MS/MS is a highly specific and sensitive tool to measure urinary methylated nucleosides for analysis of CAD. Our result has revealed that the evaluation of urinary methylated nucleosides might be helpful in the analysis of CAD by liquid chromatography/mass spectrometry. Therefore, this N-6-methyladenosine is worthy of further studies in the near future. Copyright (C) 2014 John Wiley & Sons, Ltd.

语种: 英语
所属项目编号: 2012CB720600 ; 2012CB720601
项目资助者: 973 Program
WOS记录号: WOS:000341192200004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51660
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
2.Peking Univ Third Hosp, Key Lab Mol Cardiovasc Sci, Minist Educ, Dept Cardiol, Beijing, Peoples R China
3.Peking Univ Third Hosp, Key Lab Mol Cardiovasc Sci, Minist Educ, Inst Vasc Med, Beijing, Peoples R China
4.Minist Hlth, Key Lab Mol Cardiovasc Mol Biol & Regulatory Pept, Beijing, Peoples R China

Recommended Citation:
Li, Yanru,Yu, Haiyi,Zhao, Wei,et al. Analysis of urinary methylated nucleosides of patients with coronary artery disease by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2014,28(19):2054-2058.
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