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学科主题: 基础医学
题名:
Oregonin from the Bark of Alnus japonica Restrained Ischemia-Reperfusion-Induced Mesentery Oxidative Stress by Inhibiting NADPH Oxidase Activation
作者: Nguyen Huu Tung1; Sun, Kai2; Fan, Jing-Yu2; Shoyama, Yukihiro1; Han, Jing-Yan2,3
关键词: leukocyte adhesion ; mast cell degranulation ; DHR ; p47(phox) subunit
刊名: MICROCIRCULATION
发表日期: 2014-11-01
DOI: 10.1111/micc.12147
卷: 21, 期:8, 页:688-695
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Hematology ; Peripheral Vascular Disease
研究领域[WOS]: Hematology ; Cardiovascular System & Cardiology
关键词[WOS]: RAT MESENTERY ; MAST-CELLS ; DIARYLHEPTANOIDS ; EXPRESSION ; ISCHEMIA/REPERFUSION ; PATHOPHYSIOLOGY ; DISTURBANCE ; ADHESION ; TARGETS ; INJURY
英文摘要:

ObjectiveNADPH oxidase activation results in ROS overproduction that is the pathological basis of I/R injury. This study aimed to investigate potential effects of ORG on I/R-induced ROS production in rat mesenteric microvasculature and underlying mechanisms.

MethodsMesenteric I/R in Male Wistar rats (200 similar to 250g) was induced by ligation of the mesenteric artery and vein for 10minutes followed by reperfusion for 60minutes by releasing of the occlusion. The rats were infused intravenously with or without ORG (5mg/kg per hour) 10minutes before ischemia (pretreatment) or 20minutes after reperfusion (posttreatment). The DHR fluorescence intensity on, the leukocytes adherent to, and mast cell degranulation out of mesenteric venules were determined using an intravital microscope. NADPH oxidase subunit p47(phox) membrane translocation in intestine tissues was detected by Western blotting.

ResultsPre- or posttreatment with ORG inhibited I/R-induced DHR fluorescence intensity on the venular walls and leukocytes adhesion, ORG pretreatment inhibited mast cell degranulation as well. Furthermore, the translocation of p47(phox) from cytosol to membrane was suppressed markedly by ORG after I/R.

ConclusionsThe results suggested that ORG restrained I/R-induced ROS production, which might be correlated with its inhibitive effect on NADPH activation.

语种: 英语
所属项目编号: 81270341
项目资助者: Japan Society for the Promotion of Science (JSPS) ; National Natural Science Foundation of China ; JSPS ; Ghanaian Medicinal Plans Project (JICA/JST)
WOS记录号: WOS:000344789400002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51672
Appears in Collections:基础医学院_北大医学部天士力微循环研究中心_期刊论文

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作者单位: 1.Nagasaki Int Univ, Fac Pharmaceut Sci, Nagasaki 8593298, Japan
2.Peking Univ Hlth Sci Ctr, Tasly Microcirculat Res Ctr, Beijing, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Integrat Chinese & Western Med, Beijing 100191, Peoples R China

Recommended Citation:
Nguyen Huu Tung,Sun, Kai,Fan, Jing-Yu,et al. Oregonin from the Bark of Alnus japonica Restrained Ischemia-Reperfusion-Induced Mesentery Oxidative Stress by Inhibiting NADPH Oxidase Activation[J]. MICROCIRCULATION,2014,21(8):688-695.
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