IR@PKUHSC  > 北京大学第一临床医学院  > 消化科
学科主题临床医学
Silencing of Jagged1 inhibits cell growth and invasion in colorectal cancer
Dai, Y.1,9; Wilson, G.2,3,9; Huang, B.4,9; Peng, M.4; Teng, G.1; Zhang, D.4; Zhang, R.4; Ebert, M. P. A.5; Chen, J.6; Wong, B. C. Y.7; Chan, K. W.8; George, J.2,3; Qiao, L.2,3
关键词Colorectal Cancer Notch Signaling Jagged1 Targeting Xenograft Tumors
刊名CELL DEATH & DISEASE
2014-04-01
DOI10.1038/cddis.2014.137
5
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]COLON-CANCER ; ENDOTHELIAL-CELLS ; DOWN-REGULATION ; TUMOR-GROWTH ; LUNG-CANCER ; NOTCH ; EXPRESSION ; METASTASIS ; MATRIX-METALLOPROTEINASE-9 ; ANGIOGENESIS
英文摘要

Dysregulated Notch signaling has a critical role in the tumorigenesis. Jagged1, a Notch ligand, is overexpressed in various human cancers. Recent studies revealed the involvement of Jagged1 in colorectal cancer (CRC) development. These basic studies provide a promising potential for inhibition of the Notch pathway for the treatment of CRC. Herein, we aimed to investigate the consequences of targeting Jagged1 using shRNA on CRC both in vitro and in vivo to test their potential to inhibit this key element for CRC treatment. We found that downregulation of Jagged1 with lentiviral Jagged1-shRNA resulted in decreased colon cancer cell viability in vitro, most likely mediated through reduced cell proliferation. Importantly, Jagged1 knockdown induced G(0)/G(1) phase cell cycle arrest, with reduced Cyclin D1, Cyclin E and c-Myc expression. Silencing of Jagged1 reduced the migration and invasive capacity of the colon cancer cells in vitro. Furthermore, colon cancer cells with knockdown of Jagged1 had much slower growth rate than control cells in a xenograft mouse model in vivo, with a marked downregulation of cell proliferation markers (PCNA, Ki-67, and c-Myc) and metastasis markers (MMP-2 and MMP-9). These findings rationalize a mechanistic approach to CRC treatment based on Jagged1-targeted therapeutic development.

语种英语
WOS记录号WOS:000335450400016
引用统计
被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51676
专题北京大学第一临床医学院_消化科
作者单位1.Peking Univ, Hosp 1, Dept Gastroenterol, Beijing, Peoples R China
2.Univ Sydney, Storr Liver Unit, Westmead Millennium Inst, Dept Med, Westmead, NSW 2145, Australia
3.Western Clin Sch, Westmead, NSW 2145, Australia
4.Tianjin Med Univ, Res Ctr Basic Med Sci, Minist China, Tianjin, Peoples R China
5.Tianjin Med Univ, Dept Immunol, Minist China, Key Lab Immune Microenvironment & Dis, Tianjin, Peoples R China
6.Tech Univ, Klinikum Rechts Isar, Dept Med 2, Munich, Germany
7.Univ Queensland, Sch Biomed Sci, Brisbane, Qld, Australia
8.Univ Hong Kong, Dept Med, Pokfulam, Hong Kong, Peoples R China
9.Univ Hong Kong, Dept Pathol, Pokfulam, Hong Kong, Peoples R China
推荐引用方式
GB/T 7714
Dai, Y.,Wilson, G.,Huang, B.,et al. Silencing of Jagged1 inhibits cell growth and invasion in colorectal cancer[J]. CELL DEATH & DISEASE,2014,5.
APA Dai, Y..,Wilson, G..,Huang, B..,Peng, M..,Teng, G..,...&Qiao, L..(2014).Silencing of Jagged1 inhibits cell growth and invasion in colorectal cancer.CELL DEATH & DISEASE,5.
MLA Dai, Y.,et al."Silencing of Jagged1 inhibits cell growth and invasion in colorectal cancer".CELL DEATH & DISEASE 5(2014).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Dai, Y.]的文章
[Wilson, G.]的文章
[Huang, B.]的文章
百度学术
百度学术中相似的文章
[Dai, Y.]的文章
[Wilson, G.]的文章
[Huang, B.]的文章
必应学术
必应学术中相似的文章
[Dai, Y.]的文章
[Wilson, G.]的文章
[Huang, B.]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。