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学科主题: 临床医学
题名:
Combination of retinal pigment epithelium cell-conditioned medium and photoreceptor outer segments stimulate mesenchymal stem cell differentiation toward a functional retinal pigment epithelium cell phenotype
作者: Huang, Chen1,2; Zhang, Jing1; Ao, Mingxin1; Li, Ying1; Zhang, Chun1; Xu, Yonggen1; Li, Xuemin1; Wang, Wei1
关键词: MESENCHYMAL STEM CELLS ; RETINAL PIGMENT EPITHELIUM ; CONDITIONED MEDIUM ; PHOTORECEPTOR OUTER SEGMENT ; DIFFERENTIATION
刊名: JOURNAL OF CELLULAR BIOCHEMISTRY
发表日期: 2012-02-01
DOI: 10.1002/jcb.23383
卷: 113, 期:2, 页:590-598
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: RETINITIS-PIGMENTOSA ; SUBRETINAL SPACE ; PHAGOCYTOSIS ; RPE ; EXPRESSION ; DAMAGE ; MOUSE ; INTEGRIN ; POLARITY ; INVITRO
英文摘要:

Recent studies have suggested that bone marrow-derived mesenchymal stem cells (BMMSCs) are capable of retinal tissue-specific differentiation but not retinal pigment epithelium (RPE) cell-specific differentiation. Photoreceptor outer segments (POS) contribute to RPE development and maturation. However, there has been no standard culture system that fosters the differentiation of BMMSCs into mature RPE cells in vitro. In this study, we investigated if the soluble factors from RPE cells and POS could differentiate BMMSCs into cells having a phenotype characteristic of RPE cells. Rat BMMSCs were separately co-cultured with RPE cells, or they were exposed to either control medium, RPE cell-conditioned medium (RPECM), POS, or a combination of RPECM and POS (RPECM-POS). After 7 days, the cells were analyzed for morphology and the expression of RPE markers (cytokeratin 8, CRALBP, and RPE65) to assess the RPE differentiation. Significantly higher pigment accumulation and increased protein expression of the three markers were seen in cells cultured in RPECM-POS than in other treated cultures. Furthermore, the RPECM-POS-treated cultures displayed ultrastructural features typical of RPE cells, expressed RPE cell functional proteins, and had the capability to phagocytose POS. Together, theses results suggest the combination of RPECM and POS stimulate BMMSCs differentiation toward a functional RPE phenotype. Our results provide the foundation for a new route to RPE regenerative therapy involving BMMSCs. Future work isolating the active agent in RPECM and POS would be useful in therapies for RPE diseases or in developing appropriately pre-differentiated BMMSCs for tissue-engineered RPE reconstruction. J. Cell. Biochem. 113: 590598, 2012. (C) 2011 Wiley Periodicals, Inc.

语种: 英语
所属项目编号: 30672284 ; 20080430288
项目资助者: National Natural Science Foundation of China ; China Postdoctoral Science Foundation
WOS记录号: WOS:000298843200025
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51678
Appears in Collections:北京大学第三临床医学院_眼科_期刊论文

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作者单位: 1.Peking Univ Third Hosp, Dept Ophthalmol, Beijing 100191, Peoples R China
2.Peking Univ Third Hosp, Med Res Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Huang, Chen,Zhang, Jing,Ao, Mingxin,et al. Combination of retinal pigment epithelium cell-conditioned medium and photoreceptor outer segments stimulate mesenchymal stem cell differentiation toward a functional retinal pigment epithelium cell phenotype[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2012,113(2):590-598.
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