IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
High Prevalence of Plasmid-Mediated Quinolone Resistance Genes qnr and aac(6 ′)-Ib-cr in Clinical Isolates of Enterobacteriaceae from Nine Teaching Hospitals in China
Yang, Hong1,2; Chen, Hongbin1; Yang, Qiwen1; Chen, Minjun1; Wang, Hui1
刊名ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
2008-12-01
DOI10.1128/AAC.00830-08
52期:12页:4268-4273
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology ; Pharmacology & Pharmacy
研究领域[WOS]Microbiology ; Pharmacology & Pharmacy
关键词[WOS]SPECTRUM BETA-LACTAMASES ; ESCHERICHIA-COLI ; KLEBSIELLA-PNEUMONIAE ; TRANSFERABLE PLASMID ; MODIFYING ENZYME ; UNITED-STATES ; EPIDEMIOLOGY ; EXPRESSION ; EMERGENCE
英文摘要

Quinolone resistance is an emerging problem in China. To investigate the prevalence of the plasmid-mediated quinolone resistance genes qnr and aac(6′)-Ib-cr, a total of 265 clinical isolates of Escherichia coli, Klebsiella pneumoniae, Citrobacter freundii, and Enterobacter cloacae with ciprofloxacin MICs of >= 0.25 mu g/ml were screened at nine teaching hospitals in China. The qnrA, qnrB, qnrS, and aac(6′)-Ib genes were detected by PCR. The aac(6′)-Ib-cr gene was further identified by digestion with BtsCI and/or direct sequencing. The qnr gene was present in significantly smaller numbers of isolates with cefotaxime MICs of <2 mu g/ml than isolates with higher MICs (>= 2.0 mu g/ml) (20.6% and 42.1%, respectively; P < 0.05). aac(6′)-Ib-cr was present in 17.0% of the isolates tested, and 7.9% of the isolates carried both the qnr and the aac( 6′)-Ib-cr genes. Among the isolates with cefotaxime MICs of (>= 2.0 mu g/ml, qnr and aac(6′)-Ib-cr were present in 65.7% and 8.6% of E. cloacae isolates, respectively; 65.5% and 21.8% of K. pneumoniae isolates, respectively; 63.3% and 26.7% of C. freundii isolates, respectively; and 6.5% and 16.9% of E. coli isolates, respectively. The 20 transconjugants showed 16- to 128-fold increases in ciprofloxacin MICs, 14 showed 16- to 2,000-fold increases in cefotaxime MICs, and 5 showed 8- to 32-fold increases in cefoxitin MICs relative to those of the recipient due to the cotransmission of bla(CTX-M- 14), bla(CTX-M-3), bla(DHA-1), bla(SHV-2), and bla(SHV-12) with the qnr and aac(6′)-Ib-cr genes. Southern hybridization analysis showed that these genes were located on large plasmids of different sizes (53 to 193 kb). These findings indicate the high prevalence of qnr and aac(6′)-Ib-cr in members of the family Enterobacteriaceae and the widespread dissemination of multidrug resistance in China.

语种英语
WOS记录号WOS:000261029800008
Citation statistics
Cited Times:60[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51683
Collection北京大学深圳医院
作者单位1.Chinese Acad Med Sci, Peking Union Med Coll, Dept Clin Lab, Beijing 100730, Peoples R China
2.Peking Univ, Shenzhen Hosp, Dept Clin Lab, Shenzhen 518036, Peoples R China
Recommended Citation
GB/T 7714
Yang, Hong,Chen, Hongbin,Yang, Qiwen,et al. High Prevalence of Plasmid-Mediated Quinolone Resistance Genes qnr and aac(6 &prime;)-Ib-cr in Clinical Isolates of Enterobacteriaceae from Nine Teaching Hospitals in China[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY,2008,52(12):4268-4273.
APA Yang, Hong,Chen, Hongbin,Yang, Qiwen,Chen, Minjun,&Wang, Hui.(2008).High Prevalence of Plasmid-Mediated Quinolone Resistance Genes qnr and aac(6 ′)-Ib-cr in Clinical Isolates of Enterobacteriaceae from Nine Teaching Hospitals in China.ANTIMICROBIAL AGENTS AND CHEMOTHERAPY,52(12),4268-4273.
MLA Yang, Hong,et al."High Prevalence of Plasmid-Mediated Quinolone Resistance Genes qnr and aac(6 ′)-Ib-cr in Clinical Isolates of Enterobacteriaceae from Nine Teaching Hospitals in China".ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 52.12(2008):4268-4273.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Yang, Hong]'s Articles
[Chen, Hongbin]'s Articles
[Yang, Qiwen]'s Articles
百度学术
百度学术Similar articles in
[Yang, Hong]'s Articles
[Chen, Hongbin]'s Articles
[Yang, Qiwen]'s Articles
必应学术
必应学术Similar articles in
[Yang, Hong]'s Articles
[Chen, Hongbin]'s Articles
[Yang, Qiwen]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.