IR@PKUHSC  > 北京大学基础医学院  > 细胞生物学系
学科主题基础医学
Enhancement of gefitinib-induced growth inhibition by Marsdenia tenacissima extract in non-small cell lung cancer cells expressing wild or mutant EGFR
Han, Shu-Yan1; Ding, Hui-Rong2; Zhao, Wei3; Teng, Fei1; Li, Ping-Ping1
关键词Marsdenia Tenacissima Extract (Mte) Gefitinib Non-small Cell Lung Cancer (Nsclc) Combination Egfr Related Pathway
刊名BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
2014-05-22
DOI10.1186/1472-6882-14-165
14
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Integrative & Complementary Medicine
研究领域[WOS]Integrative & Complementary Medicine
关键词[WOS]FACTOR RECEPTOR ; C-MET ; MUTATIONS ; LINES ; SENSITIVITY ; COMBINATION ; ERLOTINIB ; SURVIVAL ; KINASES ; PATHWAY
英文摘要

Background: Non-small cell lung cancer (NSCLC) expressed high levels of epidermal growth factor receptor (EGFR). Gefitinib (Iressa) has demonstrated clinical efficacy in NSCLC patients harboring EGFR mutations or refractory to chemotherapy. However, most of NSCLC patients are with wild type EGFR, and showed limited response to gefitinib. Therefore, to develop new effective therapeutic interventions for NSCLC is still required. Our previous study showed Marsdenia tenacissima extract (MTE) restored gefitinib efficacy in the resistant NSCLC cells, but whether MTE acts in the gefitinib-sensitive NSCLC cells is the same as it in the resistant one is unknown.

Methods: Dose response curves for gefitinib and MTE were generated for two sensitive NSCLC cell lines with mutant or wild type EGFR status. Three different sequential combinations of MTE and gefitinib on cell growth were evaluated using IC50 and Combination Index approaches. The flow cytometric method was used to detect cell apoptosis and cell cycle profile. The impact of MTE combined with gefitinib on cell molecular network response was studied by Western blotting.

Results: Unlike in the resistant NSCLC cells, our results revealed that low cytotoxic dose of MTE (8 mg/ml) combined gefitinib with three different schedules synergistically or additively enhanced the growth inhibition of gefitinib. Among which, MTE -> MTE + gefitinib treatment was the most effective one. MTE markedly prompted cell cycle arrest and apoptosis caused by gefitinib both in EGFR mutant (HCC827) and wild type of NSCLC cells (H292). The Western blotting results showed that MTE -> MTE + gefitinib treatment further enhanced the suppression of gefitinib on cell growth and apoptosis pathway such as ERK1/2 and PI3K/Akt/mTOR. This combination also blocked the activation of EGFR and c-Met which have cross-talk with each other. Unlike in gefitinib-resistant NSCLC cells, MTE alone also demonstrated certain unexpected modulation on EGFR related cell signal pathways in the sensitive cells.

Conclusion: Our results suggest that MTE is a promising herbal medicine to improve gefitinib efficacy in NSCLC regardless of EGFR status. However, why MTE acted differently between gefitinib-sensitive and -resistant NSCLC cells needs a further research.

语种英语
WOS记录号WOS:000336858900002
项目编号7112027 ; 20090450009 ; JJ2010-17
资助机构Beijing Municipal Natural Science Foundation ; China Postdoctoral Science Foundation ; Beijing Municipal Traditional Chinese Medicine Technology Foundation
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51687
专题北京大学基础医学院_细胞生物学系
北京大学临床肿瘤学院_中心实验室
作者单位1.Cent Lab Biochem & Mol Biol, Haidian Dist, Peoples R China
2.Peking Univ, Canc Hosp & Inst, Dept Integrat Chinese & Western Med, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China
3.Peking Univ, Canc Hosp & Inst, Dept Cell Biol, Beijing 100142, Peoples R China
推荐引用方式
GB/T 7714
Han, Shu-Yan,Ding, Hui-Rong,Zhao, Wei,et al. Enhancement of gefitinib-induced growth inhibition by Marsdenia tenacissima extract in non-small cell lung cancer cells expressing wild or mutant EGFR[J]. BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,2014,14.
APA Han, Shu-Yan,Ding, Hui-Rong,Zhao, Wei,Teng, Fei,&Li, Ping-Ping.(2014).Enhancement of gefitinib-induced growth inhibition by Marsdenia tenacissima extract in non-small cell lung cancer cells expressing wild or mutant EGFR.BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,14.
MLA Han, Shu-Yan,et al."Enhancement of gefitinib-induced growth inhibition by Marsdenia tenacissima extract in non-small cell lung cancer cells expressing wild or mutant EGFR".BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE 14(2014).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Han, Shu-Yan]的文章
[Ding, Hui-Rong]的文章
[Zhao, Wei]的文章
百度学术
百度学术中相似的文章
[Han, Shu-Yan]的文章
[Ding, Hui-Rong]的文章
[Zhao, Wei]的文章
必应学术
必应学术中相似的文章
[Han, Shu-Yan]的文章
[Ding, Hui-Rong]的文章
[Zhao, Wei]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。