IR@PKUHSC  > 北京大学基础医学院  > 免疫学系
学科主题基础医学
The CKLF1-C19 peptide attenuates allergic lung inflammation by inhibiting CCR3- and CCR4-mediated chemotaxis in a mouse model of asthma
Tian, L.1,2; Li, W.3; Wang, J.3,4; Zhang, Y.1,2; Zheng, Y.1,2; Qi, H.1,2; Guo, X.1,2; Zhang, Y.1,2; Ma, D.1,2; Shen, H.3; Wang, Y.1,2
关键词asthma CCR3 CCR4 CKLF1 peptide
刊名ALLERGY
2011-02-01
DOI10.1111/j.1398-9995.2010.02478.x
66期:2页:287-297
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Allergy ; Immunology
研究领域[WOS]Allergy ; Immunology
关键词[WOS]CHEMOKINE-LIKE FACTOR-1 ; MACROPHAGE-DERIVED CHEMOKINE ; UNRESTRAINED GUINEA-PIGS ; AIRWAY MUCUS PRODUCTION ; CHRONIC FUNGAL ASTHMA ; PULMONARY-FIBROSIS ; EOTAXIN EXPRESSION ; MOLECULAR-CLONING ; FUNCTIONAL LIGAND ; HUMAN EOSINOPHIL
英文摘要

P>Background:

Human chemokine-like factor 1 (CKLF1) is a functional ligand for human CCR4, which is highly expressed on Th2 lymphocytes and plays an important role in the pathogenesis of asthma. The expression and function of CKLF1 are associated with asthma. The CKLF1 C-terminal peptides C19 and C27 also interact with human CCR4. Albeit with weaker chemotactic activity, C19 can inhibit chemotaxis induced by both CKLF1 and CCL17. Here, we explore whether C19 can act as an antagonist in the development of asthma.

Methods:

A mouse model of asthma and in vitro and in vivo chemotaxis assays were used.

Results:

Using a mouse model of asthma, we demonstrate here that C19 reduces airway eosinophilia, lung inflammation and airway hyperresponsiveness; in contrast, C27 has little effect on these parameters. The inhibitory effects of C19 on CCR4-mediated chemotaxis could be observed in human Th2 lymphocytes and in the splenocytes from ovalbumin-sensitized mice. Furthermore, we show that C19 can inhibit CCL11-induced chemotaxis of mouse eosinophils and human CCR3-transfected or mouse Ccr3-transfected HEK293 cells. In vivo chemotaxis assays revealed that C19 and C27 can reduce CCL11-mediated recruitment of eosinophils into the peritoneal cavity and that this inhibitory effect is stronger for C19 than for C27.

Conclusions:

Thus, C19 can attenuate airway eosinophilia and lung inflammation by inhibiting CCR3- and CCR4-mediated chemotaxis in a mouse model of asthma. Given its ability to inhibit human CCR3- and CCR4-meditated chemotaxis, C19 has great therapeutic potential for use in the treatment and control of allergic asthma.

语种英语
WOS记录号WOS:000285923500018
项目编号2006AA02A305 ; 2009ZX09103-724 ; 30872292 ; 90813025 ; 30825019
资助机构National High-Tech Research and Development Program of China ; National Key New Drug Creation Program of China ; National Natural Science Foundation of China
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51698
专题北京大学基础医学院_免疫学系
北京大学第三临床医学院_眼科
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Med Immunol, Beijing 100191, Peoples R China
2.Peking Univ, Human Dis Genom Ctr, Beijing 100191, Peoples R China
3.Zhejiang Univ, Affiliated Hosp 2, Dept Resp Med, Inst Resp Dis,Sch Med, Hangzhou, Zhejiang, Peoples R China
4.Hangzhou First Peoples Hosp, Work Dept Resp Med, Hangzhou, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714
Tian, L.,Li, W.,Wang, J.,et al. The CKLF1-C19 peptide attenuates allergic lung inflammation by inhibiting CCR3- and CCR4-mediated chemotaxis in a mouse model of asthma[J]. ALLERGY,2011,66(2):287-297.
APA Tian, L..,Li, W..,Wang, J..,Zhang, Y..,Zheng, Y..,...&Wang, Y..(2011).The CKLF1-C19 peptide attenuates allergic lung inflammation by inhibiting CCR3- and CCR4-mediated chemotaxis in a mouse model of asthma.ALLERGY,66(2),287-297.
MLA Tian, L.,et al."The CKLF1-C19 peptide attenuates allergic lung inflammation by inhibiting CCR3- and CCR4-mediated chemotaxis in a mouse model of asthma".ALLERGY 66.2(2011):287-297.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Allergy-2011.pdf(816KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Tian, L.]的文章
[Li, W.]的文章
[Wang, J.]的文章
百度学术
百度学术中相似的文章
[Tian, L.]的文章
[Li, W.]的文章
[Wang, J.]的文章
必应学术
必应学术中相似的文章
[Tian, L.]的文章
[Li, W.]的文章
[Wang, J.]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Allergy-2011.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。