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学科主题: 临床医学
题名:
Coexpression of vascular endothelial growth factor and its receptor KDR on gastric adenocarcinoma MGC803 cell line and stimulation of exogenous VEGF165 to MGC803 cells
作者: Tian, XJ1; Meng, L1; Shou, CC1; Dong, ZW1
关键词: tumor cells ; vascular endothelial growth factor ; VEGF receptor ; coexpression ; proliferation
刊名: SCIENCE IN CHINA SERIES C-LIFE SCIENCES
发表日期: 2000-02-01
卷: 43, 期:1, 页:88-95
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biology
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
关键词[WOS]: FLT-1
英文摘要:

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF), is an angiogenic factor playing an important role in tumor growth. VEGF/VPF interacts with endothelial cells by way of two high-affinity receptor tyrosine kinases: flt-1 and KDR. The vast majority of published studies have described expression of the VPF/VEGF receptors specifically in endothelial cells. To elucidate the further function of VEGF in solid tumor development, the coexpression of VEGF and KDR in gastric adenocarcinoma MGC803 cell lines was shown by reverse transcription polymerase chain reaction (RT-PCR). The MGC803 tumor cells could also be strongly immunostained for KDR by immunocytochemistry. It was further demonstrated that exogenous VEGF(165) can stimulate the MGC803 cell growth in both dose-dependent and time-dependent manners by H-3-thymidine incorporation. Furthermore, anti-VEGF(165) monoclonal antibody and anti-KDR monoclonal antibody could dose-dependently block the VEGF(165)-induced cell growth. These results provided new evidence that VEGF could cause autocrine stimulation to the proliferation of gastric adenocarcinoma cells.

语种: 英语
WOS记录号: WOS:000085514800012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51703
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Beijing Med Univ, Beijing Inst Canc Res, Sch Oncol, Beijing 100034, Peoples R China
2.Chinese Acad Med Sci, Inst Canc Res, Beijing 100021, Peoples R China

Recommended Citation:
Tian, XJ,Meng, L,Shou, CC,et al. Coexpression of vascular endothelial growth factor and its receptor KDR on gastric adenocarcinoma MGC803 cell line and stimulation of exogenous VEGF165 to MGC803 cells[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2000,43(1):88-95.
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