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Bog Bilberry Anthocyanin Extract Improves Motor Functional Recovery by Multifaceted Effects in Spinal Cord Injury
Wang, Jun1; Ma, Chuan1; Rong, Wei1; Jing, Hao2; Hu, Xing1; Liu, Xiaoguang1; Jiang, Liang1; Wei, Feng1; Liu, Zhongjun1
关键词Spinal Cord Injury Anthocyanin Remyelination Inflammation Glial Scar
刊名NEUROCHEMICAL RESEARCH
2012-12-01
DOI10.1007/s11064-012-0883-1
37期:12页:2814-2825
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Neurosciences
资助者China Agricultural University ; Natural Science Foundation of China ; China Agricultural University ; Natural Science Foundation of China
研究领域[WOS]Biochemistry & Molecular Biology ; Neurosciences & Neurology
关键词[WOS]AXON REGENERATION ; GLIAL SCAR ; ASTROCYTES ; DISEASE ; CELLS ; PROLIFERATION ; INFLAMMATION ; PERFORMANCE ; BLUEBERRY ; DEFICITS
英文摘要

The aim of this study was to determine the therapeutic efficiency of bog bilberry anthocyanin extract (BBAE) treatment starting 1 d after spinal cord injury (SCI) in rats and to investigate the underlying mechanism. The BBAE contained cyanidin-3-glucoside, malvidin-3-galactoside and malvidin-3-glucoside. SCI models were induced using the weight-drop method in Sprague-Dawley rats and additionally with sham group (laminectomy only). The animals were divided into four groups: vehicle-treated group; 10 mg/kg BBAE-treated group; 20 mg/kg BBAE-treated group; sham group. BBAE-treated or vehicle-treated group was administered orally at one day after SCI and then daily for 8 weeks. Locomotor functional recovery was assessed during the 8 weeks post operation period by performing a Basso, Beattie, and Bresnahan (BBB) locomotor score test. At the end of study, the animals were killed, and 1.5 cm segments of spinal cord encompassing the injury site were removed for immunohistochemistry, histopathological and western blotting analysis. Immunohistochemistry for GFAP, aggrecan, neurocan and NeuN was used to assess the degree of astrocytic glial scar formation and neuron survival. Immunohistochemistry and western blotting analysis for TNF-alpha, IL-6, IL-1 beta was used to evaluate the anti-inflammation effect of BBAE. To evaluate its inhibition effect on the astrocytes, we performed the MTT assay and immunohistochemistry for Ki67 in vitro. Results show that the BBAE-treated animals showed significantly better locomotor functional recovery, neuron death and smaller glial scar formation after spinal cord injury in vivo. In addition, BBAE administration could inhibit astrocyte proliferation in vivo and vitro. Therefore, BBAE may be useful as a promising therapeutic agent for SCI.

语种英语
所属项目编号58441-06
资助者China Agricultural University ; Natural Science Foundation of China ; China Agricultural University ; Natural Science Foundation of China
WOS记录号WOS:000311537300019
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51723
专题北京大学第三临床医学院_骨科
作者单位1.Peking Univ, Hosp 3, Dept Orthoped, Beijing 100191, Peoples R China
2.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Wang, Jun,Ma, Chuan,Rong, Wei,et al. Bog Bilberry Anthocyanin Extract Improves Motor Functional Recovery by Multifaceted Effects in Spinal Cord Injury[J]. NEUROCHEMICAL RESEARCH,2012,37(12):2814-2825.
APA Wang, Jun.,Ma, Chuan.,Rong, Wei.,Jing, Hao.,Hu, Xing.,...&Liu, Zhongjun.(2012).Bog Bilberry Anthocyanin Extract Improves Motor Functional Recovery by Multifaceted Effects in Spinal Cord Injury.NEUROCHEMICAL RESEARCH,37(12),2814-2825.
MLA Wang, Jun,et al."Bog Bilberry Anthocyanin Extract Improves Motor Functional Recovery by Multifaceted Effects in Spinal Cord Injury".NEUROCHEMICAL RESEARCH 37.12(2012):2814-2825.
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