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学科主题: 临床医学
题名:
Septin 7 is required for orderly meiosis in mouse oocytes
作者: Li, Sen1,2; Ou, Xiang-Hong1; Wei, Liang1; Wang, Zhen-Bo1; Zhang, Qing-Hua1; Ouyang, Ying-Chun1; Hou, Yi1; Schatten, Heide3; Sun, Qing-Yuan1
关键词: Septin 7 ; meiosis ; overexpression ; oocyte ; mouse
刊名: CELL CYCLE
发表日期: 2012-09-01
DOI: 10.4161/cc.21553
卷: 11, 期:17, 页:3211-3218
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: MAMMALIAN SEPTINS ; SACCHAROMYCES-CEREVISIAE ; CHROMOSOME CONGRESSION ; BUDDING YEAST ; FILAMENT FORMATION ; GTPASE ACTIVITY ; CYTOKINESIS ; MICROTUBULES ; ORGANIZATION ; CHECKPOINT
英文摘要:

Septin 7 is a conserved GTP - binding protein. In this study, we examined the localization and functions of Septin 7 during mouse oocyte meiotic maturation. Immunofluorescent analysis showed that intrinsic Septin 7 localized to the spindles from the pro-MI stage to the MII stage. Knockdown of Septin 7 by siRNA microinjection caused abnormal spindles and affected extrusion of the first polar body. Septin 7 mRNA tagged with myc was injected into GV stage oocytes to overexpress Septin 7. Overexpressed Myc-Septin 7 localized to the spindle and beneath the plasma membrane displaying long filaments. Fluorescence intensity of spindle alpha-tubulin in myc-Septin 7-injected oocytes was weaker than that of the control group, demonstrating that Septin 7 may influence recruitment of alpha-tubulin to spindles. MII oocytes injected with myc-Septin 7 exhibited abnormal chromosome alignment, and parthenogenetic activation failed to allow extrusion of the second polar body, suggesting that overexpression of Septin 7 may affect extrusion of the polar body by disturbing the alignment of chromosomes and regulating alpha-tubulin recruitment to spindles. In summary, Septin 7 may regulate meiotic cell cycle progression by affecting microtubule cytoskeletal dynamics in mouse oocytes.

语种: 英语
所属项目编号: 2012CB944404 ; 2011CB944501 ; 30930065
项目资助者: National Basic Research Program of China ; National Natural Science Foundation of China
WOS记录号: WOS:000308750600017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51734
Appears in Collections:北京大学第二临床医学院_计划生育与生殖医学科_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China
2.Peking Univ, Peoples Hosp, Reprod Med Ctr, Beijing 100871, Peoples R China
3.Univ Missouri, Dept Vet Pathobiol, Columbia, MO USA

Recommended Citation:
Li, Sen,Ou, Xiang-Hong,Wei, Liang,et al. Septin 7 is required for orderly meiosis in mouse oocytes[J]. CELL CYCLE,2012,11(17):3211-3218.
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