|Expression of HIWI in human esophageal squamous cell carcinoma is significantly associated with poorer prognosis|
|He, Wei1,2,3; Wang, Zhihui1,3; Wang, Qi4; Fan, Qingxia2; Shou, Chengcao5; Wang, Junsheng6; Giercksky, Karl-Erik3,7; Nesland, Jahn M.1,3; Suo, Zhenhe1,3|
|WOS标题词||Science & Technology|
|关键词[WOS]||CANCER STEM-CELLS ; TUMOR-RELATED DEATH ; SELF-RENEWAL ; PIWI ; GENE ; RISK ; MACHINERY ; ZEBRAFISH ; PROTEINS ; HOMOLOG|
Background: HIWI, the human homologue of Piwi family, is present in CD34(+) hematopoietic stem cells and germ cells, but not in well-differentiated cell populations, indicating that HIWI may play an impotent role in determining or maintaining stemness of these cells. That HIWI expression has been detected in several type tumours may suggest its association with clinical outcome in cancer patients.
Methods: With the methods of real-time PCR, western blot, immunocytochemistry and immunohistochemistry, the expression of HIWI in three esophageal squamous cancer cell lines KYSE70, KYSE140 and KYSE450 has been characterized. Then, we investigated HIWI expression in a series of 153 esophageal squamous cell carcinomas using immunohistochemistry and explored its association with clinicopathological features.
Results: The expression of HIWI was observed in tumour cell nuclei or/and cytoplasm in 137 (89.5%) cases, 16 (10.5%) cases were negative in both nuclei and cytoplasm. 86 (56.2%) were strongly positive in cytoplasm, while 49 (32.0%) were strongly positive in nuclei. The expression level of HIWI in cytoplasm of esophageal cancer cells was significantly associated with histological grade (P = 0.011), T stage (P = 0.035), and clinic outcome (P < 0.001), while there was no correlation between the nuclear HIWI expression and clinicopathological features.
Conclusion: The expression of HIWI in the cytoplasm of esophageal cancer cells is significantly associated with higher histological grade, clinical stage and poorer clinical outcome, indicating its possible involvement in cancer development.
|作者单位||1.Oslo Univ Hosp, Norwegian Radium Hosp, Div Pathol, N-0310 Oslo, Norway|
2.Zhengzhou Univ, Affiliated Hosp 1, Dept Oncol, Zhengzhou 450052, Peoples R China
3.Univ Oslo, Norwegian Radium Hosp, Fac Med, Fac Div, N-0316 Oslo, Norway
4.Dalian Med Univ, Hosp Affiliated 2, Dept Resp Med, Dalian 116023, Peoples R China
5.Peking Univ, Sch Oncol, Beijing Inst Canc Res, Dept Biochem & Mol Biol, Beijing 100142, Peoples R China
6.Anyang Tumor Hosp, Dept Oncol, Anyang 455000, Peoples R China
7.Oslo Univ Hosp, Norwegian Radium Hosp, Dept Surg, N-0310 Oslo, Norway
|He, Wei,Wang, Zhihui,Wang, Qi,et al. Expression of HIWI in human esophageal squamous cell carcinoma is significantly associated with poorer prognosis[J]. BMC CANCER,2009,9.|
|APA||He, Wei.,Wang, Zhihui.,Wang, Qi.,Fan, Qingxia.,Shou, Chengcao.,...&Suo, Zhenhe.(2009).Expression of HIWI in human esophageal squamous cell carcinoma is significantly associated with poorer prognosis.BMC CANCER,9.|
|MLA||He, Wei,et al."Expression of HIWI in human esophageal squamous cell carcinoma is significantly associated with poorer prognosis".BMC CANCER 9(2009).|