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学科主题临床医学
Activated focal adhesion kinase involved in adhesion and migration of vascular smooth muscle cells stimulated by fibronectin
Yin, H; Wang, LH; Huo, Y; Peng, X; Xia, CF; Tang, CS
关键词Focal Adhesion Kinase Vascular Smooth Muscle Cells Antisense Oligodeoxynucleotides Adhesion Migration
刊名CHINESE MEDICAL JOURNAL
2002-04-01
115期:4页:494-497
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]PROTEIN-KINASES
英文摘要

Objective To study the effects of focal adhesion kinase (FAK) phosphorylation on smooth muscle cells (SMCs) adhesion and migration stimulated by fibronectin.

Methods Adhesion and migration of cultured SMCs were stimulated by different concentrations of fibronectin (FN), FAK and its phosphorylation were detected by immunoprecipitation and Western blot. FAK antisense oligodeoxynucleotides (ODNs) were transfected into SMCs by cationic lipid to investigate its modulatory effects on tyrosine phosphorylation. SMCs adhesion and migration were also measured by morphological enumeration and modified Boyden Chambers, respectively.

Results FAK were expressed when SMCs adhesion and migration were successfully simulated by different concentrations of FN. FAK phosphorylation were detected only at 20 mug/ml FN or more. FAK antisense ODNs were transfected efficiently by cationic lipid and FAK phosphorylation was inhibited substantially. The SMCs migration rate in the 5 - 60 mug/ml FN groups was reduced by 17.89% - 27.67%. Cell migration stimulated by FN at 10, 20, 40 and 60 mug/ml were reduced by 23.26%, 21.63%, 19.31% and 17.88%, respectively (P < 0.05).

Conclusions FAK phosphorylation and FAK-mediated signal transduction play important roles in SMCs adhesion and migration stimulated by ECM. The process can be inhibited effectively by FAK antisense ODNs.

语种英语
WOS记录号WOS:000175635200004
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51753
专题北京大学第一临床医学院_心血管内科
北京大学口腔医学院_外科
作者单位Peking Univ, Hosp 1, Dept Cardiol, Beijing 100034, Peoples R China
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Yin, H,Wang, LH,Huo, Y,et al. Activated focal adhesion kinase involved in adhesion and migration of vascular smooth muscle cells stimulated by fibronectin[J]. CHINESE MEDICAL JOURNAL,2002,115(4):494-497.
APA Yin, H,Wang, LH,Huo, Y,Peng, X,Xia, CF,&Tang, CS.(2002).Activated focal adhesion kinase involved in adhesion and migration of vascular smooth muscle cells stimulated by fibronectin.CHINESE MEDICAL JOURNAL,115(4),494-497.
MLA Yin, H,et al."Activated focal adhesion kinase involved in adhesion and migration of vascular smooth muscle cells stimulated by fibronectin".CHINESE MEDICAL JOURNAL 115.4(2002):494-497.
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