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学科主题: 临床医学
题名:
Activated focal adhesion kinase involved in adhesion and migration of vascular smooth muscle cells stimulated by fibronectin
作者: Yin, H; Wang, LH; Huo, Y; Peng, X; Xia, CF; Tang, CS
关键词: focal adhesion kinase ; vascular smooth muscle cells ; antisense oligodeoxynucleotides ; adhesion ; migration
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2002-04-01
卷: 115, 期:4, 页:494-497
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: PROTEIN-KINASES
英文摘要:

Objective To study the effects of focal adhesion kinase (FAK) phosphorylation on smooth muscle cells (SMCs) adhesion and migration stimulated by fibronectin.

Methods Adhesion and migration of cultured SMCs were stimulated by different concentrations of fibronectin (FN), FAK and its phosphorylation were detected by immunoprecipitation and Western blot. FAK antisense oligodeoxynucleotides (ODNs) were transfected into SMCs by cationic lipid to investigate its modulatory effects on tyrosine phosphorylation. SMCs adhesion and migration were also measured by morphological enumeration and modified Boyden Chambers, respectively.

Results FAK were expressed when SMCs adhesion and migration were successfully simulated by different concentrations of FN. FAK phosphorylation were detected only at 20 mug/ml FN or more. FAK antisense ODNs were transfected efficiently by cationic lipid and FAK phosphorylation was inhibited substantially. The SMCs migration rate in the 5 - 60 mug/ml FN groups was reduced by 17.89% - 27.67%. Cell migration stimulated by FN at 10, 20, 40 and 60 mug/ml were reduced by 23.26%, 21.63%, 19.31% and 17.88%, respectively (P < 0.05).

Conclusions FAK phosphorylation and FAK-mediated signal transduction play important roles in SMCs adhesion and migration stimulated by ECM. The process can be inhibited effectively by FAK antisense ODNs.

语种: 英语
WOS记录号: WOS:000175635200004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51753
Appears in Collections:北京大学第一临床医学院_心血管内科_期刊论文

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作者单位: Peking Univ, Hosp 1, Dept Cardiol, Beijing 100034, Peoples R China

Recommended Citation:
Yin, H,Wang, LH,Huo, Y,et al. Activated focal adhesion kinase involved in adhesion and migration of vascular smooth muscle cells stimulated by fibronectin[J]. CHINESE MEDICAL JOURNAL,2002,115(4):494-497.
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