IR@PKUHSC  > 北京大学基础医学院  > 病原生物学系
学科主题基础医学
Pseudomonas aeruginosa isolates of distinct sub-genotypes exhibit similar potential of antimicrobial resistance by drugs exposure
Liu, Zhen-Hong1,2; Xu, Yan2; Duo, Li-Bo3; Liu, Yu4; Xu, Zhao-Zhen5; Burns, Jane L.6,7; Liu, Gui-Rong2; Yang, Bao-Feng1; Liu, Shu-Lin2,8,9
关键词Pseudomonas aeruginosa Sub-genotypes Antimicrobial resistance
刊名ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
2013-04-01
DOI10.1007/s10482-012-9862-4
103期:4页:797-807
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology
资助者Harbin Medical University ; National Natural Science Foundation of China ; Natural Science Foundation of Heilongjiang Province of China ; Harbin Medical University ; National Natural Science Foundation of China ; Natural Science Foundation of Heilongjiang Province of China
研究领域[WOS]Microbiology
关键词[WOS]CYSTIC-FIBROSIS PATIENTS ; RIBOSOMAL-RNA GENE ; SALMONELLA-TYPHI ; CHLAMYDOMONAS-EUGAMETOS ; OPPORTUNISTIC PATHOGEN ; GENOME ; ENDONUCLEASE ; SEQUENCE ; INTRON ; CONSERVATION
英文摘要

Pseudomonas aeruginosa, a wide-spread opportunistic pathogen, often complicates clinical treatments due to its resistance to a large variety of antimicrobials, especially in immune compromised patients, occasionally leading to death. However, the resistance to antimicrobials varies greatly among the P. aeruginosa isolates, which raises a question on whether some sub-lineages of P. aeruginosa might have greater potential to develop antimicrobial resistance than others. To explore this question, we divided 160 P. aeruginosa isolates collected from cities of USA and China into distinct genotypes using I-CeuI, a special endonuclease that had previously been proven to reveal phylogenetic relationships among bacteria reliably due to the highly conserved 26-bp recognition sequence. We resolved 10 genotypes by I-CeuI analysis and further divided them into 82 sub-genotypes by endonuclease cleavage with SpeI. Eight of the 10 genotypes contained both multi-drug resistant (MDR) and less resistant isolates based on comparisons of their antimicrobial resistance profiles (ARPs). When the less resistant or susceptible isolates from different genotypes were exposed to eight individual antimicrobials, they showed similar potential to become resistant with minor exceptions. This is to our knowledge the first report to examine correlations between phylogenetic sub-lineages of P. aeruginosa and their potential to become resistant to antimicrobials. This study further alerts the importance and urgency of antimicrobial abuse control.

语种英语
所属项目编号HCXB2009020 ; NSFC309 70078
资助者Harbin Medical University ; National Natural Science Foundation of China ; Natural Science Foundation of Heilongjiang Province of China ; Harbin Medical University ; National Natural Science Foundation of China ; Natural Science Foundation of Heilongjiang Province of China
WOS记录号WOS:000316145900011
Citation statistics
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51762
Collection北京大学基础医学院_病原生物学系
作者单位1.Seattle Childrens Hosp, Div Infect Dis, Seattle, WA USA
2.Univ Washington, Seattle, WA 98195 USA
3.Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB, Canada
4.Harbin Med Univ, Dept Pharmacol, Harbin 150081, Peoples R China
5.Harbin Med Univ, Genom Res Ctr, Harbin 150081, Peoples R China
6.Harbin Med Univ, Clin Lab, Affiliated Hosp 2, Harbin 150081, Peoples R China
7.Harbin Med Univ, Clin Lab, Affiliated Hosp 4, Harbin 150081, Peoples R China
8.Harbin Med Univ, Clin Lab, Affiliated Hosp 1, Harbin 150081, Peoples R China
9.Peking Univ, Dept Microbiol, Hlth Sci Ctr, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Liu, Zhen-Hong,Xu, Yan,Duo, Li-Bo,et al. Pseudomonas aeruginosa isolates of distinct sub-genotypes exhibit similar potential of antimicrobial resistance by drugs exposure[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2013,103(4):797-807.
APA Liu, Zhen-Hong.,Xu, Yan.,Duo, Li-Bo.,Liu, Yu.,Xu, Zhao-Zhen.,...&Liu, Shu-Lin.(2013).Pseudomonas aeruginosa isolates of distinct sub-genotypes exhibit similar potential of antimicrobial resistance by drugs exposure.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,103(4),797-807.
MLA Liu, Zhen-Hong,et al."Pseudomonas aeruginosa isolates of distinct sub-genotypes exhibit similar potential of antimicrobial resistance by drugs exposure".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 103.4(2013):797-807.
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