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学科主题临床医学
Presence of Stem/Progenitor Cells in the Rat Penis
Lin, Guiting1; Alwaal, Amjad1; Zhang, Xiaoyu1,2,3; Wang, Jianwen1,4; Wang, Lin1,2,3; Li, Huixi1,2,3; Wang, Guifang1; Ning, Hongxiu1; Lin, Ching-Shwun1; Xin, Zhongcheng1,2,3; Lue, Tom F.1
刊名STEM CELLS AND DEVELOPMENT
2015-01-15
DOI10.1089/scd.2014.0360
24期:2页:264-270
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering ; Hematology ; Medicine, Research & Experimental ; Transplantation
资助者U.S. National Institutes of Health ; U.S. National Institutes of Health
研究领域[WOS]Cell Biology ; Hematology ; Research & Experimental Medicine ; Transplantation
关键词[WOS]LABEL-RETAINING CELLS ; STEM-CELLS ; ERECTILE FUNCTION ; PROGENITOR CELLS ; DIFFERENTIATION ; PROLIFERATION ; BRDU ; DISEASE ; SKIN
英文摘要

Tissue resident stem cells are believed to exist in every organ, and their identification is commonly done using a combination of immunostaining for putative stem cell markers and label-retaining cell (LRC) strategy. In this study, we employed these approaches to identify potential stem cells in the penis. Newborn rats were intraperitoneally injected with thymidine analog, 5-ethynyl-2-deoxyuridine (EdU), and their penis was harvested at 7 h, 3 days, 1 week, and 4 weeks. It was processed for EdU stains and immunofluorescence staining for stem cell markers A2B5, PCNA, and c-kit. EdU-positive cells were counted for each time point and co-localized with each stem cell marker, then isolated and cultured in vitro followed by their characterization using flowcytometry and immunofluorescence. At 7 h post-EdU injection, 410 +/- 105.3 penile corporal cells were labeled in each cross-section (similar to 28%). The number of EdU-positive cells at 3 days increased to 536 +/- 115.6, while their percentage dropped to 25%. Progressively fewer EdU-positive cells were present in the sacrificed rat penis at longer time points (1 and 4 weeks). They were mainly distributed in the subtunic and perisinusoidal spaces, and defined as subtunic penile progenitor cells (STPCs) and perisinusoidal penile progenitor cells (PPCs). These cells expressed c-kit, A2B5, and PCNA. After culturing in vitro, only similar to 0.324% corporal cells were EdU-labeled LRCs and expressed A2B5/PCNA. Therefore, labeling of penis cells by EdU occurred randomly, and label retaining was not associated with expression of c-kit, A2B5, or PCNA. The penile LRCs are mainly distributed within the subtunic and perisinusoidal space.

语种英语
所属项目编号DK045370
资助者U.S. National Institutes of Health ; U.S. National Institutes of Health
WOS记录号WOS:000347542000012
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51784
专题北京大学第一临床医学院_泌尿外科
作者单位1.Peking Univ, Hosp 1, Dept Urol, Beijing 100871, Peoples R China
2.Peking Univ, Inst Urol, Beijing 100871, Peoples R China
3.Univ Calif San Francisco, Sch Med, Dept Urol, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
4.Capital Med Univ, Sch Med, Dept Urol, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Lin, Guiting,Alwaal, Amjad,Zhang, Xiaoyu,et al. Presence of Stem/Progenitor Cells in the Rat Penis[J]. STEM CELLS AND DEVELOPMENT,2015,24(2):264-270.
APA Lin, Guiting.,Alwaal, Amjad.,Zhang, Xiaoyu.,Wang, Jianwen.,Wang, Lin.,...&Lue, Tom F..(2015).Presence of Stem/Progenitor Cells in the Rat Penis.STEM CELLS AND DEVELOPMENT,24(2),264-270.
MLA Lin, Guiting,et al."Presence of Stem/Progenitor Cells in the Rat Penis".STEM CELLS AND DEVELOPMENT 24.2(2015):264-270.
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