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Dux4 induces cell cycle arrest at G1 phase through upregulation of p21 expression
Xu, Hongliang1; Wang, Zhaoxia1; Jin, Suqin1; Hao, Hongjun1; Zheng, Lemin2; Zhou, Boda3; Zhang, Wei1; Lv, He1; Yuan, Yun1
关键词Fshd Dux4 P21 Cell Cycle
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2014-03-28
DOI10.1016/j.bbrc.2014.02.105
446期:1页:235-240
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]FACIOSCAPULOHUMERAL MUSCULAR-DYSTROPHY ; DNA-REPLICATION ; GENE ; P53 ; PROLIFERATION ; INHIBITOR ; SP1 ; LOCUS ; DIFFERENTIATION ; P21(CIP1/WAF1)
英文摘要

It has been implicated that Dux4 plays crucial roles in development of facioscapulohumeral dystrophy. But the underlying myopathic mechanisms and related down-stream events of this retrogene were far from clear. Here, we reported that overexpression of Dux4 in a cell model TE671 reduced cell proliferation rate, and increased G1 phase accumulation. We also determined the impact of Dux4 on p53/p21 signal pathway, which controls the checkpoint in cell cycle progression. Overexpression of Dux4 increased p21 mRNA and protein level, while expression of p53, phospho-p53 remained unchanged. Silencing p21 rescued Dux4 mediated proliferation defect and cell cycle arrest. Furthermore, we demonstrated that enhanced Dux4 expression increased p21 promoter activity and elevated expression of Sp1 transcription factor. Mutation of Sp1 binding site decreased dux4 induced p21 promoter activation. Chromatin immunoprecipitation (ChIP) assays confirmed the Dux4-induced binding of Sp1 to p21 promoter in vivo. These results suggest that Dux4 might induce proliferation inhibition and G1 phase arrest through upregulation of p21. (c) 2014 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000334654900039
项目编号2011ZX09307-001-07
资助机构Twelfth Five-year Plan of China
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51795
专题北京大学第一临床医学院_神经内科
北京大学基础医学院
北京大学第三临床医学院_心血管内科
作者单位1.Peking Univ, Hosp 1, Dept Neurol, Beijing 100034, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Inst Cardiovasc Sci,Educ Minist,Minist Hlth, Key Lab Mol Cardiovasc Sci,Key Lab Cardiovasc Mol, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Dept Cardiol, Beijing 100191, Peoples R China
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Xu, Hongliang,Wang, Zhaoxia,Jin, Suqin,et al. Dux4 induces cell cycle arrest at G1 phase through upregulation of p21 expression[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2014,446(1):235-240.
APA Xu, Hongliang.,Wang, Zhaoxia.,Jin, Suqin.,Hao, Hongjun.,Zheng, Lemin.,...&Yuan, Yun.(2014).Dux4 induces cell cycle arrest at G1 phase through upregulation of p21 expression.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,446(1),235-240.
MLA Xu, Hongliang,et al."Dux4 induces cell cycle arrest at G1 phase through upregulation of p21 expression".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 446.1(2014):235-240.
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