IR@PKUHSC  > 北京大学第一临床医学院  > 普通外科
学科主题临床医学
Stimulation of stellate cells by injured acinar cells: a model of acute pancreatitis induced by alcohol and fat (VLDL)
Siech, Marco1; Zhou, Zhengfei2,5; Zhou, Shaoxia3; Bair, Bernd2; Alt, Andreas4; Hamm, Stefan2; Gross, Hans3; Mayer, Jens2; Beger, Hans G.2; Tian, Xiaodong2; Kornmann, Marko2; Bachem, Max G.3
关键词Ethanol Free Radicals Pancreas
刊名AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
2009-12-01
DOI10.1152/ajpgi.90468.2008
297期:6页:G1163-G1171
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology ; Physiology
研究领域[WOS]Gastroenterology & Hepatology ; Physiology
关键词[WOS]PATHOLOGICAL LIVER-INJURY ; COLLAGEN TYPE-I ; MATRIX SYNTHESIS ; NONOXIDATIVE METABOLISM ; OXIDATIVE STRESS ; RAT PANCREAS ; JUICE EDEMA ; ETHANOL ; PROLIFERATION ; IDENTIFICATION
英文摘要

Siech M, Zhou Z, Zhou S, Bair B, Alt A, Hamm S, Gross H, Mayer J, Beger HG, Tian X, Kornmann M, Bachem MG. Stimulation of stellate cells by injured acinar cells: a model of acute pancreatitis induced by alcohol and fat (VLDL). Am J Physiol Gastrointest Liver Physiol 297: G1163-G1171, 2009. First published September 24, 2009; doi: 10.1152/ajpgi.90468.2008.-Mechanisms leading to acute pancreatitis after a fat-enriched meal combined with excess alcohol are incompletely understood. We have studied the effects of alcohol and fat (VLDL) on pancreatic acinar cell (PAC) function, oxidative stress, and repair mechanisms by pancreatic stellate cells (PSC) leading to fibrogenesis. To do so, PAC (rat) were isolated and cultured up to 24 h. Ethanol and/or VLDL were added to PAC. We measured PAC function (amylase, lipase), injury (lactic dehydrogenase), apoptosis (TUNEL, Apo2.7, annexin V binding), oxidative stress, and lipid peroxidation (conjugated dienes, malondialdehyde, chemoluminescence); we also measured PSC proliferation (bromodeoxyuridine incorporation), matrix synthesis (immunofluorescence of collagens and fibronectin, fibronectin immunoassay), and fatty acids in PAC supernatants (gas chromatography). Within 6 h, cultured PAC degraded and hydrolyzed VLDL completely. VLDL alone (50 mu g/ml) and in combination with alcohol (0.2, 0.5, and 1% vol/vol) induced PAC injury (LDL, amylase, and lipase release) within 2 h through generation of oxidative stress. Depending on the dose of VLDL and alcohol, apoptosis and/or necrosis were induced. Antioxidants (Trolox, Probucol) reduced the cytotoxic effect of alcohol and VLDL. Supernatants of alcohol/VLDL-treated PAC stimulated stellate cell proliferation and extracellular matrix synthesis. We concluded that, in the presence of lipoproteins, alcohol induces acinar cell injury. Our results provide a biochemical pathway for the clinical observation that a fat-enriched meal combined with excess alcohol consumption can induce acinar cell injury (acute pancreatitis) followed by repair mechanisms (proliferation and increased matrix synthesis in PSC).

语种英语
WOS记录号WOS:000272151900013
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51796
专题北京大学第一临床医学院_普通外科
作者单位1.Univ Ulm, Dept Gen & Transplantat Surg, Aalen, Germany
2.Univ Ulm, Dept Clin Chem, Aalen, Germany
3.Univ Ulm, Dept Legal Med, Aalen, Germany
4.Univ Hosp Ulm, Teaching Hosp, Dept Gen & Vasc Surg, Ostalb Klinikum Aalen, D-73430 Aalen, Germany
5.Peking Univ, Hosp 1, Dept Gen Surg, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Siech, Marco,Zhou, Zhengfei,Zhou, Shaoxia,et al. Stimulation of stellate cells by injured acinar cells: a model of acute pancreatitis induced by alcohol and fat (VLDL)[J]. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY,2009,297(6):G1163-G1171.
APA Siech, Marco.,Zhou, Zhengfei.,Zhou, Shaoxia.,Bair, Bernd.,Alt, Andreas.,...&Bachem, Max G..(2009).Stimulation of stellate cells by injured acinar cells: a model of acute pancreatitis induced by alcohol and fat (VLDL).AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY,297(6),G1163-G1171.
MLA Siech, Marco,et al."Stimulation of stellate cells by injured acinar cells: a model of acute pancreatitis induced by alcohol and fat (VLDL)".AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY 297.6(2009):G1163-G1171.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Siech, Marco]的文章
[Zhou, Zhengfei]的文章
[Zhou, Shaoxia]的文章
百度学术
百度学术中相似的文章
[Siech, Marco]的文章
[Zhou, Zhengfei]的文章
[Zhou, Shaoxia]的文章
必应学术
必应学术中相似的文章
[Siech, Marco]的文章
[Zhou, Zhengfei]的文章
[Zhou, Shaoxia]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。