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IR@PKUHSC  > 北京大学第一临床医学院  > 普通外科  > 期刊论文
学科主题: 临床医学
题名:
Stimulation of stellate cells by injured acinar cells: a model of acute pancreatitis induced by alcohol and fat (VLDL)
作者: Siech, Marco1; Zhou, Zhengfei2,5; Zhou, Shaoxia3; Bair, Bernd2; Alt, Andreas4; Hamm, Stefan2; Gross, Hans3; Mayer, Jens2; Beger, Hans G.2; Tian, Xiaodong2; Kornmann, Marko2; Bachem, Max G.3
关键词: ethanol ; free radicals ; pancreas
刊名: AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
发表日期: 2009-12-01
DOI: 10.1152/ajpgi.90468.2008
卷: 297, 期:6, 页:G1163-G1171
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology ; Physiology
研究领域[WOS]: Gastroenterology & Hepatology ; Physiology
关键词[WOS]: PATHOLOGICAL LIVER-INJURY ; COLLAGEN TYPE-I ; MATRIX SYNTHESIS ; NONOXIDATIVE METABOLISM ; OXIDATIVE STRESS ; RAT PANCREAS ; JUICE EDEMA ; ETHANOL ; PROLIFERATION ; IDENTIFICATION
英文摘要:

Siech M, Zhou Z, Zhou S, Bair B, Alt A, Hamm S, Gross H, Mayer J, Beger HG, Tian X, Kornmann M, Bachem MG. Stimulation of stellate cells by injured acinar cells: a model of acute pancreatitis induced by alcohol and fat (VLDL). Am J Physiol Gastrointest Liver Physiol 297: G1163-G1171, 2009. First published September 24, 2009; doi: 10.1152/ajpgi.90468.2008.-Mechanisms leading to acute pancreatitis after a fat-enriched meal combined with excess alcohol are incompletely understood. We have studied the effects of alcohol and fat (VLDL) on pancreatic acinar cell (PAC) function, oxidative stress, and repair mechanisms by pancreatic stellate cells (PSC) leading to fibrogenesis. To do so, PAC (rat) were isolated and cultured up to 24 h. Ethanol and/or VLDL were added to PAC. We measured PAC function (amylase, lipase), injury (lactic dehydrogenase), apoptosis (TUNEL, Apo2.7, annexin V binding), oxidative stress, and lipid peroxidation (conjugated dienes, malondialdehyde, chemoluminescence); we also measured PSC proliferation (bromodeoxyuridine incorporation), matrix synthesis (immunofluorescence of collagens and fibronectin, fibronectin immunoassay), and fatty acids in PAC supernatants (gas chromatography). Within 6 h, cultured PAC degraded and hydrolyzed VLDL completely. VLDL alone (50 mu g/ml) and in combination with alcohol (0.2, 0.5, and 1% vol/vol) induced PAC injury (LDL, amylase, and lipase release) within 2 h through generation of oxidative stress. Depending on the dose of VLDL and alcohol, apoptosis and/or necrosis were induced. Antioxidants (Trolox, Probucol) reduced the cytotoxic effect of alcohol and VLDL. Supernatants of alcohol/VLDL-treated PAC stimulated stellate cell proliferation and extracellular matrix synthesis. We concluded that, in the presence of lipoproteins, alcohol induces acinar cell injury. Our results provide a biochemical pathway for the clinical observation that a fat-enriched meal combined with excess alcohol consumption can induce acinar cell injury (acute pancreatitis) followed by repair mechanisms (proliferation and increased matrix synthesis in PSC).

语种: 英语
WOS记录号: WOS:000272151900013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51796
Appears in Collections:北京大学第一临床医学院_普通外科_期刊论文

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作者单位: 1.Univ Ulm, Dept Gen & Transplantat Surg, Aalen, Germany
2.Univ Ulm, Dept Clin Chem, Aalen, Germany
3.Univ Ulm, Dept Legal Med, Aalen, Germany
4.Univ Hosp Ulm, Teaching Hosp, Dept Gen & Vasc Surg, Ostalb Klinikum Aalen, D-73430 Aalen, Germany
5.Peking Univ, Hosp 1, Dept Gen Surg, Beijing 100871, Peoples R China

Recommended Citation:
Siech, Marco,Zhou, Zhengfei,Zhou, Shaoxia,et al. Stimulation of stellate cells by injured acinar cells: a model of acute pancreatitis induced by alcohol and fat (VLDL)[J]. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY,2009,297(6):G1163-G1171.
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