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Molecular cloning and characterization of a human gene involved in transcriptional regulation of hTERT
Tang, ZW; Zhao, YJ; Mei, F; Yang, SM; Li, X; Lv, JJ; Hou, L; Zhang, B
关键词hTERT telomerase transcription regulation yeast one-hybrid
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2004-11-26
DOI10.1016/j.bbrc.2004.09.201
324期:4页:1324-1332
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]TELOMERASE-REVERSE-TRANSCRIPTASE ; CATALYTIC SUBUNIT HTERT ; DEPENDENT PROTEIN-KINASE ; TUMOR-CELLS ; MESSENGER-RNA ; IN-VIVO ; PROMOTER ; IDENTIFICATION ; ACTIVATION ; CANCER
英文摘要

Human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase in human, has been identified as the rate-limiting factor in telomerase activity, but its detailed mechanism of transcription regulation remains unclear. In this investigation, a novel human gene telomerase transcriptional elements-interacting factor (TEIF) was isolated from HeLa cell based on hTERT promoter yeast one-hybrid assay. TEIF has a 2358-bp open reading frame encoding a predicted protein of 786 amino acids, which is functionally conserved in general eukaryotic species. The transcription of TEIF was detected in cells and expressed an 86-kDa native protein, distributing mainly in cellular nuclei. Its interaction with hTERT promoter was identified and the DNA binding potential was credited to C-terminus of TEIF. Transfection of TEIF into HeLa cells presented marked transactivation of hTERT promoter and stimulated both endogenous hTERT transcription and telomerase activities. These results suggest that TEIF protein might be a transcription regulator and take part in the activation of hTERT. (C) 2004 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000224939100025
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51797
专题基础医学院_病理学系
作者单位Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China
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GB/T 7714
Tang, ZW,Zhao, YJ,Mei, F,et al. Molecular cloning and characterization of a human gene involved in transcriptional regulation of hTERT[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2004,324(4):1324-1332.
APA Tang, ZW.,Zhao, YJ.,Mei, F.,Yang, SM.,Li, X.,...&Zhang, B.(2004).Molecular cloning and characterization of a human gene involved in transcriptional regulation of hTERT.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,324(4),1324-1332.
MLA Tang, ZW,et al."Molecular cloning and characterization of a human gene involved in transcriptional regulation of hTERT".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 324.4(2004):1324-1332.
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