IR@PKUHSC  > 北京大学第一临床医学院  > 感染疾病科
学科主题临床医学
Rapid, Simple, and Sensitive Detection of Anaplasma phagocytophilum by Loop-Mediated Isothermal Amplification of the msp2 Gene
Pan, Lei1; Zhang, Lijuan1; Wang, Guiqiang2; Liu, Qinghui3; Yu, Yanyan2; Wang, Shiwen1; Yu, Huilan1; He, Jing4
刊名JOURNAL OF CLINICAL MICROBIOLOGY
2011-12-01
DOI10.1128/JCM.01085-11
49期:12页:4117-4120
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology
资助者National Basic Research Program of China (973 Program) ; National Key Science and Technology Projects of China ; National Basic Research Program of China (973 Program) ; National Key Science and Technology Projects of China
研究领域[WOS]Microbiology
关键词[WOS]HUMAN GRANULOCYTIC ANAPLASMOSIS ; ORIENTIA-TSUTSUGAMUSHI ; IDENTIFICATION ; EHRLICHIOSIS ; RICKETTSIA ; DIAGNOSIS ; LAMP
英文摘要

Anaplasma phagocytophilum is the causative agent of human granulocytic anaplasmosis, which is prevalent throughout China. In this study, we describe a rapid, simple, and sensitive loop-mediated isothermal amplification (LAMP) assay targeting the msp2 gene of A. phagocytophilum that is ideal for application in rural areas in China. This assay has the potential to detect A. phagocytophilum early in infection as an alternative to existing methods. A total of 42 suspected cases of infection with A. phagocytophilum, 15 serologically confirmed and 27 probable cases, were analyzed by the msp2 LAMP assay. To validate the accuracy of LAMP, previously established nested-PCR and real-time PCR assays were utilized. The sensitivity of LAMP was 25 copies per reaction (approximately 1,250 copies per ml blood) for A. phagocytophilum, and the assay did not detect false positives among 27 members of the order Rickettsiales and 17 common clinical pathogens. To evaluate the clinical applicability of the LAMP assay, a total of 42 clinical samples were examined. A positive LAMP result was obtained for 12 of the confirmed cases and for 14 of 27 suspected cases, while only 1 confirmed case and 3 cases (2 confirmed cases and 1 suspected case) were detected by nested PCR and real-time PCR, respectively. The LAMP assay described in this study demonstrated a high level of sensitivity comparable with that of nested PCR and real-time PCR for the detection of A. phagocytophilum. This LAMP assay is a valuable method for rapid, cost-effective, and simple detection of A. phagocytophilum in the rural areas of China.

语种英语
所属项目编号2010CB530200 ; 2010CB530206 ; 2009ZX10004-203 ; 2008ZX10004-008
资助者National Basic Research Program of China (973 Program) ; National Key Science and Technology Projects of China ; National Basic Research Program of China (973 Program) ; National Key Science and Technology Projects of China
WOS记录号WOS:000298113400016
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51802
专题北京大学第一临床医学院_感染疾病科
作者单位1.Laizhou First Peoples Hosp, Laizhou, Shandong, Peoples R China
2.302 Hosp PLA, Dept Infect Dis, Beijing, Peoples R China
3.China CDC, Dept Rickettsiol, Natl Inst Communicable Dis Control & Prevent, Beijing 102206, Peoples R China
4.Peking Univ, Hosp 1, Dept Infect Dis, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Pan, Lei,Zhang, Lijuan,Wang, Guiqiang,et al. Rapid, Simple, and Sensitive Detection of Anaplasma phagocytophilum by Loop-Mediated Isothermal Amplification of the msp2 Gene[J]. JOURNAL OF CLINICAL MICROBIOLOGY,2011,49(12):4117-4120.
APA Pan, Lei.,Zhang, Lijuan.,Wang, Guiqiang.,Liu, Qinghui.,Yu, Yanyan.,...&He, Jing.(2011).Rapid, Simple, and Sensitive Detection of Anaplasma phagocytophilum by Loop-Mediated Isothermal Amplification of the msp2 Gene.JOURNAL OF CLINICAL MICROBIOLOGY,49(12),4117-4120.
MLA Pan, Lei,et al."Rapid, Simple, and Sensitive Detection of Anaplasma phagocytophilum by Loop-Mediated Isothermal Amplification of the msp2 Gene".JOURNAL OF CLINICAL MICROBIOLOGY 49.12(2011):4117-4120.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Pan, Lei]的文章
[Zhang, Lijuan]的文章
[Wang, Guiqiang]的文章
百度学术
百度学术中相似的文章
[Pan, Lei]的文章
[Zhang, Lijuan]的文章
[Wang, Guiqiang]的文章
必应学术
必应学术中相似的文章
[Pan, Lei]的文章
[Zhang, Lijuan]的文章
[Wang, Guiqiang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。