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Protective effect of tubuloside B on TNF alpha-induced apoptosis in neuronal cells
Deng, M1; Zhao, JY1; Ju, XD1; Tu, PF1; Jiang, Y1; Li, ZB1
关键词Tubuloside b Apoptosis Tumor Necrosis Factor Sh-sy5y Cells Mitochondria Membrane Potential Reactive Oxygen Species Caspase-3 Calcium
刊名ACTA PHARMACOLOGICA SINICA
2004-10-01
25期:10页:1276-1284
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]TUMOR-NECROSIS-FACTOR ; OXIDATIVE STRESS ; NEURODEGENERATIVE DISORDERS ; CEREBRAL-ISCHEMIA ; NERVOUS-SYSTEM ; MITOCHONDRIA ; INJURY ; DEATH ; PHENYLETHANOIDS ; MACROPHAGES
英文摘要

AIM: To investigate the neuroprotective effect of tubuloside B, one of the phenylethanoids isolated from the stems of Cistanche salsa, on tumor necrosis factor-alpha (TNFalpha)-induced apoptosis in SH-SY5Y neuronal cells. METHODS: Cell viability was analyzed using MTT assay. Apoptotic cells were detected using Hoechst33342 staining, and confirmed by DNA fragmentation and flow cytometric analysis. The activity of caspase-3 was measured with special assay kit. The concentration of free intracellular calcium was determined with the probe Indo-1 by spectrometer. The level of intracellular reactive oxygen species and the potential of mitochondrial membrane were determined by laser scanning confocal microscopy (LSCM) combined with fluorescence probe H2DCFDA or JC-1 respectively. RESULTS: SH-SY5Y cells treated with TNFalpha 100 mug/L for 36 h showed typical morphological changes of apoptosis. DNA ladder could be observed by agarose gel electrophoresis. The highest percentage of apoptotic cells accumulated to 37.5 %. Following 36 h treatment with TNFalpha, accumulation of intracellular ROS and [Ca(2+)](i) and decrease in mitochondrial membrane potential were observed, and caspase-3 activity increased by about five-fold compared with controls. However, pretreatment with tubuloside B (1, 10, or 100 mg/L) for 2 h attenuated the TNFalpha-mediated apoptosis. The antiapoptotic action of tubuloside B was partially dependent on an anti-oxidative stress effects, maintain of mitochondria function, decrease of concentration of free intracellular calcium and inhibition of caspase-3 activity. CONCLUSION: Tubuloside B has the neuroprotective capacity to antagonize TNFalpha-induced apoptosis in SH-SY5Y cells and may be useful in treating some neurodegenerative diseases.

语种英语
WOS记录号WOS:000224505800006
引用统计
被引频次:32[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51814
专题北京大学第三临床医学院_骨科
北京大学药学院_天然药物学系
北京大学第一临床医学院_皮肤性病科
北京大学第三临床医学院_运动医学研究所
作者单位1.Peking Univ, Hosp 3, Dept Orthopaed, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 3, Res Ctr Occupat Med, Beijing 100083, Peoples R China
3.Peking Univ, Modern Res Ctr Tradit Chinese Med, Beijing 100083, Peoples R China
4.Chinese Acad Med Sci, Inst Mat Med, Peking Union Med Coll, Beijing 100050, Peoples R China
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Deng, M,Zhao, JY,Ju, XD,et al. Protective effect of tubuloside B on TNF alpha-induced apoptosis in neuronal cells[J]. ACTA PHARMACOLOGICA SINICA,2004,25(10):1276-1284.
APA Deng, M,Zhao, JY,Ju, XD,Tu, PF,Jiang, Y,&Li, ZB.(2004).Protective effect of tubuloside B on TNF alpha-induced apoptosis in neuronal cells.ACTA PHARMACOLOGICA SINICA,25(10),1276-1284.
MLA Deng, M,et al."Protective effect of tubuloside B on TNF alpha-induced apoptosis in neuronal cells".ACTA PHARMACOLOGICA SINICA 25.10(2004):1276-1284.
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