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Food-Animal Related Staphylococcus aureus Multidrug-Resistant ST9 Strains with Toxin Genes
He, Wenqiang1; Liu, Yuqing2; Qi, Jing2; Chen, Hongbin1; Zhao, Chunjiang1; Zhang, Feifei1; Li, Henan1; Wang, Hui1
刊名FOODBORNE PATHOGENS AND DISEASE
2013-09-01
DOI10.1089/fpd.2012.1452
10期:9页:782-788
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Food Science & Technology
研究领域[WOS]Food Science & Technology
关键词[WOS]FIELD GEL-ELECTROPHORESIS ; METHICILLIN-RESISTANT ; PIG FARMERS ; MRSA ; SWINE ; ST398 ; IDENTIFICATION ; PREVALENCE ; WORKERS ; GENOME
英文摘要

To determine whether methicillin-susceptible S. aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) are present in commercial pig farms and food products from supermarkets in China, we characterized S. aureus isolates from 250 samples associated with swine and animal-related food products in Shandong Province. The isolates were characterized by susceptibility testing, toxin gene detection, pulse-field gel electrophoresis (PFGE), multilocus sequence typing, and spa typing. MRSA were identified and typed by the staphylococcal cassette chromosome mec (SCCmec). The prevalence of S. aureus among all samples was 19.6% (49/250). MRSA and MSSA accounted for 16.7% (20/120) and 8.3% (10/120), respectively, of swine feces samples. Only MSSA was detected from swine carcass, pork, chicken, and raw milk, accounting for 15% (6/40), 10% (3/30), 20% (6/30), and 13.3% (4/30), respectively. The predominant MRSA clone was ST9-t899 SCCmecIVb/PFGE A (70.0%, 14/20). Among the MSSA isolates, ST9-t899/PFGE A was the most prevalent (27.6%), followed by ST15-t084 (17.2%), ST97-t2756 (10.3%), ST1-t127 (6.9%), and ST398-t899 (3.5%). Some lineages were found that are commonly detected in humans (e. g., ST1, ST5, ST7, ST59, ST88) or are human-specific (e. g., ST15). The toxin genes sec, seh, and enterotoxin gene cluster (egc) were significantly more prevalent among isolates of lineage ST9 (p < 0.001) compared to other lineages, and the ST9 isolates were more resistant to erythromycin, clindamycin, ciprofloxacin, chloramphenicol, and gentamicin. The same lineage was identified from different sample types, indicating circulation of the related strains within the area of study. In conclusion, swine and food products of animal origin carried S. aureus, and the predominant ST9 clone possesses a multidrug-resistance profile and a high prevalence of sec, seh, and egc enterotoxin genes.

语种英语
WOS记录号WOS:000330456900006
项目编号NCET-10-0205 ; 2012EP001002
资助机构Program for New Century Excellent Talents in University ; Key Projects in the National Science and Technology Pillar Program
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51858
专题北京大学第二临床医学院
北京大学第二临床医学院_检验科
作者单位1.Peking Univ, Peoples Hosp, Dept Clin Lab, Beijing 100044, Peoples R China
2.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan, Shandong, Peoples R China
推荐引用方式
GB/T 7714
He, Wenqiang,Liu, Yuqing,Qi, Jing,et al. Food-Animal Related Staphylococcus aureus Multidrug-Resistant ST9 Strains with Toxin Genes[J]. FOODBORNE PATHOGENS AND DISEASE,2013,10(9):782-788.
APA He, Wenqiang.,Liu, Yuqing.,Qi, Jing.,Chen, Hongbin.,Zhao, Chunjiang.,...&Wang, Hui.(2013).Food-Animal Related Staphylococcus aureus Multidrug-Resistant ST9 Strains with Toxin Genes.FOODBORNE PATHOGENS AND DISEASE,10(9),782-788.
MLA He, Wenqiang,et al."Food-Animal Related Staphylococcus aureus Multidrug-Resistant ST9 Strains with Toxin Genes".FOODBORNE PATHOGENS AND DISEASE 10.9(2013):782-788.
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