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学科主题: 口腔医学
题名:
Comparison of the biological response of osteoblasts after tension and compression
作者: Zhong, Zhe1; Zeng, Xiang-Long1; Ni, Ju-Hua2; Huang, Xiao-Feng3
刊名: EUROPEAN JOURNAL OF ORTHODONTICS
发表日期: 2013-02-01
DOI: 10.1093/ejo/cjr016
卷: 35, 期:1, 页:59-65
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: BONE-MASS ; IN-VITRO ; CELLS ; EXPRESSION ; ACTIN ; OSTEOCLASTOGENESIS ; MUTATION ; STRESS ; SYSTEM ; LIGAND
英文摘要:

The aim of this study was to investigate the difference in the biological response of osteoblasts when stretched and compressed. A cellular cyclic tension and compression apparatus (CCTCA) was designed to stretch and compress cells under the same conditions. After stretching or compressing MC3T3-E1 with continuously increased strain for 5 hours, cellular cytoskeletal modulation was detected by immunohistochemical assay with actin antibody. Real-time polymerase chain reaction was performed at 1, 3, and 5 hours to detect local factors related to bone remodelling. Statistical analysis was undertaken with analysis of variance and the Kruskal-Wallis.

Following stretching or compression for 5 hours, MC3T3-E1 attached to the culture dishes grew well. Compared with the control, the microfilaments orientated parallel with each other and were clearly observed by laser scanning confocal microscope after 5 hours of stretching. The morphology of MC3T3-E1 cells was thinner and longer than the control. However, microfilaments presented a disordered arrangement after 5 hours of compression, and the MC3T3-E1 cells decreased in size. Gene expression of Wnt10b and Lrp5 increased during tension but more in the compression groups at 1, 3, and 5 hours. The ratio of osteoprotegerin to receptor activator for nuclear factor kappa B ligand increased in the tension group compared with the control but decreased in the compression group at 5 hours.

语种: 英语
所属项目编号: 30772452
项目资助者: National Natural Sciences Foundation of the People&prime ; s Republic of China
WOS记录号: WOS:000314118800009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51859
Appears in Collections:北京大学口腔医学院_牙周科_期刊论文

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作者单位: 1.Peking Univ, Dept Orthodont, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
2.Peking Univ, Dept Biochem & Mol Biol, Beijing 100081, Peoples R China
3.Capital Med Univ, Beijing Friendship Hosp, Dept Stomatol, Beijing, Peoples R China

Recommended Citation:
Zhong, Zhe,Zeng, Xiang-Long,Ni, Ju-Hua,et al. Comparison of the biological response of osteoblasts after tension and compression[J]. EUROPEAN JOURNAL OF ORTHODONTICS,2013,35(1):59-65.
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