|Comparison of the biological response of osteoblasts after tension and compression|
|Zhong, Zhe1; Zeng, Xiang-Long1; Ni, Ju-Hua2; Huang, Xiao-Feng3|
|刊名||EUROPEAN JOURNAL OF ORTHODONTICS|
|WOS标题词||Science & Technology|
|类目[WOS]||Dentistry, Oral Surgery & Medicine|
|研究领域[WOS]||Dentistry, Oral Surgery & Medicine|
|关键词[WOS]||BONE-MASS ; IN-VITRO ; CELLS ; EXPRESSION ; ACTIN ; OSTEOCLASTOGENESIS ; MUTATION ; STRESS ; SYSTEM ; LIGAND|
The aim of this study was to investigate the difference in the biological response of osteoblasts when stretched and compressed. A cellular cyclic tension and compression apparatus (CCTCA) was designed to stretch and compress cells under the same conditions. After stretching or compressing MC3T3-E1 with continuously increased strain for 5 hours, cellular cytoskeletal modulation was detected by immunohistochemical assay with actin antibody. Real-time polymerase chain reaction was performed at 1, 3, and 5 hours to detect local factors related to bone remodelling. Statistical analysis was undertaken with analysis of variance and the Kruskal-Wallis.
Following stretching or compression for 5 hours, MC3T3-E1 attached to the culture dishes grew well. Compared with the control, the microfilaments orientated parallel with each other and were clearly observed by laser scanning confocal microscope after 5 hours of stretching. The morphology of MC3T3-E1 cells was thinner and longer than the control. However, microfilaments presented a disordered arrangement after 5 hours of compression, and the MC3T3-E1 cells decreased in size. Gene expression of Wnt10b and Lrp5 increased during tension but more in the compression groups at 1, 3, and 5 hours. The ratio of osteoprotegerin to receptor activator for nuclear factor kappa B ligand increased in the tension group compared with the control but decreased in the compression group at 5 hours.
|资助机构||National Natural Sciences Foundation of the People&prime ; s Republic of China|
|作者单位||1.Peking Univ, Dept Orthodont, Sch & Hosp Stomatol, Beijing 100081, Peoples R China|
2.Peking Univ, Dept Biochem & Mol Biol, Beijing 100081, Peoples R China
3.Capital Med Univ, Beijing Friendship Hosp, Dept Stomatol, Beijing, Peoples R China
|Zhong, Zhe,Zeng, Xiang-Long,Ni, Ju-Hua,et al. Comparison of the biological response of osteoblasts after tension and compression[J]. EUROPEAN JOURNAL OF ORTHODONTICS,2013,35(1):59-65.|
|APA||Zhong, Zhe,Zeng, Xiang-Long,Ni, Ju-Hua,&Huang, Xiao-Feng.(2013).Comparison of the biological response of osteoblasts after tension and compression.EUROPEAN JOURNAL OF ORTHODONTICS,35(1),59-65.|
|MLA||Zhong, Zhe,et al."Comparison of the biological response of osteoblasts after tension and compression".EUROPEAN JOURNAL OF ORTHODONTICS 35.1(2013):59-65.|