|Effects of LDL, cholesterol, and their oxidized forms on the precipitation kinetics of calcium phosphates|
|Wang, HP1; Feng, XJ1; Gou, BD1; Zhang, TL1; Xu, SJ1; Wang, K1|
|WOS标题词||Science & Technology|
|类目[WOS]||Medical Laboratory Technology|
|研究领域[WOS]||Medical Laboratory Technology|
|关键词[WOS]||ATHEROSCLEROTIC LESIONS ; ARTERIAL CALCIFICATION ; OXIDATIVE MODIFICATION ; OCTACALCIUM PHOSPHATE ; BONE PHOSPHOPROTEINS ; CRYSTAL-FORMATION ; SMOOTH-MUSCLE ; HYDROXYAPATITE ; ANTIOXIDANTS ; PERSPECTIVE|
Background: LDL, cholesterol, and their oxidized forms are known cardiovascular risk factors and are often found in atherosclerotic lesions of various stages. Little is known, however, about whether they are directly involved in the formation of calcium phosphate compounds.
Methods: We used the pH-stat technique to follow the kinetics of calcium phosphate precipitation at pH 7.4, 37 degreesC, and ionic strength 0.150 mol/L; in the presence or absence of LDL, oxidized LDL, cholesterol, cholestane-3beta,5alpha,6beta-triol, and cholesteryl linoleate. The precipitates were characterized by x-ray diffraction, scanning and transmission electronic microscopy coupled with energy-dispersion x-ray analysis; and inductively coupled plasma atomic emission spectroscopy.
Results: Under the experimental conditions, LDL (14.8 and 43.1 mg/L protein) had no significant effect on the precipitation kinetics. Oxidized LDL (14.8 and 43.1 mg/L protein) prolonged the nucleation phase and diminished the amount of total precipitate, and both the extent of oxidation and the concentration of the protein affected the kinetics. Cholesterol microcrystals (71.4 and 143 mg/L) made the nucleation phase shorter (300 min vs 390 min for the control), and the precipitated particles had an organic core and a shell composed, of calcium phosphates. L-a-Phosphatidylcholine vesicles (143 mg/L), cholesterol (71.4 mg/L)/phospholipid (143 mg/L) mixed vesicles, cholesteryl :linoleate (143 mg/L), and. cholestane-3beta,5alpha,6beta-triol (71.4 mg/L) prolonged the nucleation phase.
Conclusions: LDL is not involved directly in the precipitation of calcium phosphates. Oxidized LDL inhibits both nucleation- and crystal growth, possibly by attracting calcium ions in the solution and thus reducing supersaturation. Cholesterol microcrystals serve as seeds for. the precipitation of hydroxyapatite, whereas L-a-phosphatidylcholine, cholesteryl linoleate, and cholestane-3beta,5alpha,6beta-triol exhibit inhibitive effects on the nucleation of calcium phosphates. (C) 2003 American Association for Clinical Chemistry.
|作者单位||1.State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China|
2.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
|Wang, HP,Feng, XJ,Gou, BD,et al. Effects of LDL, cholesterol, and their oxidized forms on the precipitation kinetics of calcium phosphates[J]. CLINICAL CHEMISTRY,2003,49(12):2027-2036.|
|APA||Wang, HP,Feng, XJ,Gou, BD,Zhang, TL,Xu, SJ,&Wang, K.(2003).Effects of LDL, cholesterol, and their oxidized forms on the precipitation kinetics of calcium phosphates.CLINICAL CHEMISTRY,49(12),2027-2036.|
|MLA||Wang, HP,et al."Effects of LDL, cholesterol, and their oxidized forms on the precipitation kinetics of calcium phosphates".CLINICAL CHEMISTRY 49.12(2003):2027-2036.|