|In vivo detection of severity of optic nerve crush using manganese-enhanced magnetic resonance imaging in rats|
|Feng Yun1,2,3; Luo Lisha1,3; Ma Zhizhong1,2,3; Sun Xiaodong4; Hu Yuntao1,2,3|
|关键词||Manganese Manganese-enhanced Agnetic Resonance Imaging Optic Nerve Retina|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||AXONAL-TRANSPORT ; MRI ; REGENERATION ; RESOLUTION ; TOXICITY ; CONTRAST ; PATHWAY ; MODEL|
Background Traumatic optic neuropathy (TON) is one of the reasons for permanent vision loss. Currently, the clinical practices may not be sufficient for direct assessments and comprehensively determining the location and extent of the patients with optic nerve injury in traumatic optic neuropathy. Magnetic resonance imaging (MRI) provides a non-invasive option. However, rare reports have found whether the differentdegree of injury of the optic nerve can be detected by manganese-enhanced MRI (MEMRI). This study aimed to explore the efficacy of MEMRI in the visual pathway for different severity of opitic nerve injury in rats.
Methods The different injuries of mild, moderate, and heavy damages were created by modified reverse tweezer and were evaluated by counting retinal ganglion cells (RGCs) and VEP ananlysis. Sprague-Dawley (SD) rats were intravitreally injected with 2 I of 25 mmol/L MnCl2, which has been confirmed as a safe injection concentration. The contrast-to-noise ratio (CNR) of MEMRI for optic nerve enhancement at different injury levels was measured.
Results The location of the significantly decreased signal point on optic nerve (ON) was corresponding to the location we made. However, similar findings are not obvious, or even have not been observed in 28 days in each group and also in 14 days at F100 group, indicating that MEMRI could be directly intuitive positioned in the early stage on the optic nerve injury.
Conclusions The possibility of using MEMRI in optic nerve injury in a safe injection concentration of 25 mmol/L is confirmed. Therefore, it is possible to detect the severity of the optic nerve by MEMRI examination.
|项目编号||2011CB707506 ; YZZ08-9-13 ; 64508-01|
|资助机构||National Basic Research Program of China (973 Grant) ; Seed Fund from the Peking University Third Hospital ; Linghu Fund from the Peking University Third Hospital|
|作者单位||1.Peking Univ, Ctr Eye, Beijing 100191, Peoples R China|
2.Peking Univ, Hosp 3, Dept Ophthalmol, Beijing 100191, Peoples R China
3.Minist Educ, Key Lab Vis Loss & Restorat, Beijing 100191, Peoples R China
4.Shanghai Jiao Tong Univ, Peoples Hosp 1, Dept Ophthalmol, Shanghai 200080, Peoples R China
|Feng Yun,Luo Lisha,Ma Zhizhong,et al. In vivo detection of severity of optic nerve crush using manganese-enhanced magnetic resonance imaging in rats[J]. CHINESE MEDICAL JOURNAL,2014,127(3):522-527.|
|APA||Feng Yun,Luo Lisha,Ma Zhizhong,Sun Xiaodong,&Hu Yuntao.(2014).In vivo detection of severity of optic nerve crush using manganese-enhanced magnetic resonance imaging in rats.CHINESE MEDICAL JOURNAL,127(3),522-527.|
|MLA||Feng Yun,et al."In vivo detection of severity of optic nerve crush using manganese-enhanced magnetic resonance imaging in rats".CHINESE MEDICAL JOURNAL 127.3(2014):522-527.|
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