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学科主题基础医学
Molecular Analysis of Virulent Determinants of Enterovirus 71
Li, Renqing1; Zou, Qinghua2; Chen, Lijuan1; Zhang, Herun1; Wang, Yumei1
刊名PLOS ONE
2011-10-19
DOI10.1371/journal.pone.0026237
6期:10
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]MOUTH-DISEASE ; STRUCTURE PREDICTION ; SECONDARY STRUCTURE ; RNA ; POLIOVIRUS ; HAND ; FOOT ; STRAINS ; NEUROVIRULENCE ; ATTENUATION
英文摘要

Enterovirus 71 (EV71) is the most important causative agent of hand, foot and mouth disease (HFMD) in children. In most cases, it is a self-limiting illness. However some EV71 infectious cases can develop severe clinical outcomes, such as encephalitis, meningitis, poliomyelitis like paralysis, and even death. To identify the determinants of virulence, the deduced amino acid sequence of polyprotein and nucleotide sequence of 5′-NTR and 3′-NTR in 25 SC-EV71 strains (strains from severe cases) and 31 MC-EV71 strains (strains from mild cases) were analyzed. Results showed four amino acids on two positions (Gly(P710)/Gln(P710)/Arg(P710) and Glu(P729)) on the DE and EF loop of VP1, one (Lys(P930)) on the surface of protease 2A and four nucleotides on three positions (G(P272), U-P488 and A(P700)/U-P700) in the 5′-NTR region are associated with EV71 virulent phenotype. Predicted secondary structure of RNA using the consensus sequence of 5′-NTR by RNAStructure showed the mutation of nucleotide at position 488 in strain BJ08-Z004-3 (position 491 in prototype strain BrCr) can result in the discrepancy of an additional pair of nucleotides and thus change the stability of the second structure of IRES. Fragment base content analysis showed that in the region 696 to 714 bp at the 5′-NTR, where the A(P700)/U-P700 was located, the nucleotide constitution ratios differed significantly between SC-EV71 and MC-EV71 strains. In conclusion, comparative genomic analysis showed that virulence of EV71 strains are mainly determined by the amino acids on two positions of VP1, one position of protease 2A and the nucleotides on three positions in 5′-NTR.

语种英语
WOS记录号WOS:000296507500043
资助机构Chinese government
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被引频次:32[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51888
专题北京大学基础医学院_病原生物学系
北京大学基础医学院
作者单位1.Beijing Ctr Dis Control & Prevent, Beijing, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Li, Renqing,Zou, Qinghua,Chen, Lijuan,et al. Molecular Analysis of Virulent Determinants of Enterovirus 71[J]. PLOS ONE,2011,6(10).
APA Li, Renqing,Zou, Qinghua,Chen, Lijuan,Zhang, Herun,&Wang, Yumei.(2011).Molecular Analysis of Virulent Determinants of Enterovirus 71.PLOS ONE,6(10).
MLA Li, Renqing,et al."Molecular Analysis of Virulent Determinants of Enterovirus 71".PLOS ONE 6.10(2011).
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