|Prevalence of porcine endogenous retrovirus in Chinese pig breeds and in patients treated with a porcine liver cell-based bioreactor|
|Liu, Qing1; Liu, Zheng2; Dalakas, Evangelos3|
|关键词||Porcine Endogenous Retrovirus Bioartificial Liver Support System Porcine Endogenous Retrovirus-reverse Transcriptase|
|刊名||WORLD JOURNAL OF GASTROENTEROLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
AIM: To determine the prevalence of porcine endogenous retrovirus (PERV) in various pig breeds raised in China including Chinese experimental mini-pigs by PERV-reverse transcriptase (PERV-RT enzyme). Moreover, the potential for infection of PERV was investigated in patients treated with a bioreactor based on porcine liver cells (n = 3).
METHODS: Pig serum, liver and muscle cell-free supernatants were collected from various Chinese pig breeds. Porcine hepatocytes were isolated with a two-step perfusion method. Three patients with acute or chronic liver failure were treated with a bioartificial liver support system (BALSS) for 8-12 h and serum samples were collected from the patients before, immediately after and 30 d after treatment.
RESULTS: The activities of PERV-RT enzyme in pig liver and muscle cell-free supernatants were higher than in normal human controls. PERV-TR enzyme activity did not increase in patients before and after 1 mo of treatment. PERV-RT activities were not significantly different when compared with pre-treatment group (1.544 +/- 0.155576), the post-treatment groups (1.501 +/- 0.053507, 1.461 +/- 0.033808 and 1.6006667 +/- 0.01963 for 0, 14 and 30 d post-treatment, respectively, P>0.05), and normal control group (1.440 +/- 1.0641, P>0.05). RT enzyme activity in Chinese experimental mini-pigs was higher than in normal human control group (1.440 +/- 1.0641 U/mL, P<0.05), and not significantly different (P>0.05) when compared with the pig breeds except in the muscle supernatants. All the samples including muscle and liver cell supernatants from the Chinese mini-experimental pigs and the four domestic Chinese pig breeds contained PERVs.
CONCLUSION: These results suggest that the risk of PERV infection through BALSS containing porcine liver cells without immunosuppressants may be quite low. Although there were PERVs in Chinese experimental mini-pigs and porcine liver cell culture suspensions, we did not find any evidence of persistent PERV infection in patients treated with this porcine hepatocyte-based bioartificial liver. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.
|作者单位||1.Capital Univ Med Sci, Artificial Liver Treatment & Training Ctr, Beijing Youan Hosp, Beijing 100054, Peoples R China|
2.Peking Univ, Shen Zheng Hosp, Dept Gen Surg, Shenzheng 518036, Guangdong, Peoples R China
3.Univ Edinburgh, Dept Hepatol, Edinburgh EH16 4SB, Midlothian, Scotland
|Liu, Qing,Liu, Zheng,Dalakas, Evangelos. Prevalence of porcine endogenous retrovirus in Chinese pig breeds and in patients treated with a porcine liver cell-based bioreactor[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2005,11(30):4727-4730.|
|APA||Liu, Qing,Liu, Zheng,&Dalakas, Evangelos.(2005).Prevalence of porcine endogenous retrovirus in Chinese pig breeds and in patients treated with a porcine liver cell-based bioreactor.WORLD JOURNAL OF GASTROENTEROLOGY,11(30),4727-4730.|
|MLA||Liu, Qing,et al."Prevalence of porcine endogenous retrovirus in Chinese pig breeds and in patients treated with a porcine liver cell-based bioreactor".WORLD JOURNAL OF GASTROENTEROLOGY 11.30(2005):4727-4730.|