IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
Assessment of retrospective dose estimation, with fluorescence in situ hybridization (FISH), of six victims previously exposed to accidental ionizing radiation
Liu, Qing-Jie1; Lu, Xue1; Zhao, Xiao-Tao2; Feng, Jiang-Bin1; Lu, Yu-Min3; Jiang, En-Hai4,5; Zhang, Shu-Lan6; Chen, De-Qing1; Jia, Ting-Zhen6; Liang, Li6
关键词Radiation Accident Retrospective Dose Estimation Fluorescence In Situ Hybridization Background Translocation Frequency
刊名MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
2014
DOI10.1016/j.mrgentox.2013.07.016
759页:1-8
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
研究领域[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
关键词[WOS]CHROMOSOME-ABERRATION ANALYSIS ; ATOMIC-BOMB SURVIVORS ; CYTOGENETIC FOLLOW-UP ; TOOTH ENAMEL ; DICENTRIC FREQUENCIES ; BIOLOGICAL DOSIMETRY ; HUMAN-LYMPHOCYTES ; SYMMETRICAL TRANSLOCATIONS ; GAMMA-IRRADIATION ; CO-60 RADIATION
英文摘要

The present study aims to evaluate the use of the fluorescence in situ hybridization (FISH) translocation assay for retrospective dose estimation of acute accidental exposure to radiation in the past. Reciprocal translocation analysis by FISH with three whole-chromosome probes was performed on normal peripheral blood samples. Samples were irradiated with 0-5 Gy(60)Co gamma-rays in vitro, and dose-effect curves were established. FISH-based translocation analyses for six accident victims were then performed, and biological doses were estimated retrospectively by comparison with the dose-effect curves. Reconstructed doses by FISH were compared with estimated doses obtained by analysis of di-centrics performed soon after exposure, or with dose estimates from tooth-enamel electron paramagnetic resonance (EPR) data obtained at the same time as the FISH analysis. Follow-up FISH analyses for an adolescent victim were performed. Results showed that dose-effect curves established in the present study follow a linear-quadratic model, regardless of the background translocation frequency. Estimated doses according to two dose-effect curves for all six victims were similar. FISH dose estimations of three adult victims exposed to accidental radiation less than a decade prior to analysis (3, 6, or 7 years ago) were consistent with those estimated with tooth-enamel EPR measurements or analyses of di-centrics. Estimated doses of two other adult victims exposed to radiation over a decade prior to analysis (16 or 33 years ago) were underestimated and two to three times lower than the values obtained from analysis of dicentrics or tooth-enamel EPR. Follow-up analyses of the adolescent victim showed that doses estimated by FISH analysis decrease rapidly over time. Therefore, the accuracy of dose estimates by FISH is acceptable only when analysis is performed less than 7 years after exposure. Measurements carried out more than a decade after exposure through FISH analysis resulted in underestimation of the biological doses compared with values obtained through analysis of di-centrics and tooth-enamel EPR. (C) 2013 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000331505200001
项目编号30570551 ; 81172593 ; 98-2-46
资助机构National Natural Science Foundation of China ; Scientific Research Fund of Ministry of Health, People&prime ; s Republic of China
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51892
专题北京大学第三临床医学院
北京大学第三临床医学院_肿瘤治疗中心
作者单位1.Henan Inst Occupat Med, Zhengzhou 450052, Peoples R China
2.Chinese Ctr Dis Control & Prevent, Natl Inst Radiol Protect, China CDC Key Lab Radiol Protect & Nucl Emergency, Beijing 100088, Peoples R China
3.Capital Med Univ, Beijing Chao Yang Hosp, Dept Cardiol, Beijing 100020, Peoples R China
4.Chinese Acad Med Sci, Inst Radiat Med, Tianjin 300020, Peoples R China
5.Peking Union Med Coll, Tianjin 300020, Peoples R China
6.Peking Univ, Hosp 3, Dept Tumor Chemotherapy & Radiat Sickness, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Liu, Qing-Jie,Lu, Xue,Zhao, Xiao-Tao,et al. Assessment of retrospective dose estimation, with fluorescence in situ hybridization (FISH), of six victims previously exposed to accidental ionizing radiation[J]. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,2014,759:1-8.
APA Liu, Qing-Jie.,Lu, Xue.,Zhao, Xiao-Tao.,Feng, Jiang-Bin.,Lu, Yu-Min.,...&Liang, Li.(2014).Assessment of retrospective dose estimation, with fluorescence in situ hybridization (FISH), of six victims previously exposed to accidental ionizing radiation.MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,759,1-8.
MLA Liu, Qing-Jie,et al."Assessment of retrospective dose estimation, with fluorescence in situ hybridization (FISH), of six victims previously exposed to accidental ionizing radiation".MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 759(2014):1-8.
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