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Immunohistochemical analysis of the expression of FATE/BJ-HCC-2 antigen in normal and malignant tissues
Yang, XA1; Dong, XY1; Qiao, H1; Wang, YD1; Peng, JR1; Li, Y1; Pang, XW1; Tian, C1; Chen, WF1
关键词cancer/testis antigen FATE/BJ-HCC-2 FATE/BJ-HCC-2 antigen hepatocellular carcinoma immunohistochemical analysis
刊名LABORATORY INVESTIGATION
2005-02-01
DOI10.1038/labinvest.3700220
85期:2页:205-213
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental ; Pathology
研究领域[WOS]Research & Experimental Medicine ; Pathology
关键词[WOS]POLYMERASE-CHAIN-REACTION ; HEPATOCELLULAR-CARCINOMA ; PERIPHERAL-BLOOD ; MELANOMA-CELLS ; TUMOR-ANTIGENS ; CANCER/TESTIS ANTIGENS ; MONOCLONAL-ANTIBODIES ; REVERSE-TRANSCRIPTASE ; IMMUNE-RESPONSES ; REACTION ASSAY
英文摘要

FATE/BJ-HCC-2 is a newly identified cancer/ testis (CT) antigen, which was detected in tumor tissues and testis. As previous studies of FATE/BJ-HCC-2 expression pattern were mainly based on messenger RNA (mRNA) analysis, it is necessary to investigate its actual protein expression pattern in tumor tissues for the evaluation of its application value. In this study, we produced specific polyclonal antibody (pAb) to the recombinant FATE/ BJ-HCC-2 protein and analyzed the FATE/BJ-HCC-2 antigen expression in normal and malignant tissues by the immunohistochemical approach. The results showed that there was no detectable FATE/BJ-HCC-2 antigen expressed in normal tissues except testis. In hepatocellular carcinoma (HCC) tissues, the FATE/ BJ-HCC-2 antigen was detected in 20% (7/35) specimens. All samples that expressed the FATE/ BJ-HCC-2 antigen were of poorly or moderately differentiated HCC. The stained antigen was located in the cytoplasm and the staining pattern showed heterogeneity from focal to more than 40% of the tumor cells. The FATE/BJ-HCC-2 antigen was also expressed in other tumor tissues. The results of [H-3] thymidine incorporation showed that FATE/ BJ-HCC-2 protein enhanced tumor cell proliferation after transfection of FATE/ BJ-HCC-2 gene in HCC cell line ( P<0.01). This effect could be specifically blocked by anti-FATE/BJ-HCC-2 pAb. Serological screening showed that the antibody specific to the FATE/ BJ-HCC-2 antigen was detected in 7.7% (4/52) patients. Notably, the four positive patients bore poorly or moderately differentiated HCC. FATE/ BJ-HCC-2 mRNA transcript was detected in the peripheral blood mononuclear cells (PBMCs) of 46.67% patients whose resected HCC tissue samples were positive for FATE/ BJ-HCC-2 mRNA, which implicated tumor cell dissemination in blood circulation and may relate to the metastasis of HCC. Thus, FATE/ BJ-HCC-2 may be a valuable candidate CT antigen for polyvalent vaccines in tumor immunotherapy and an assisting diagnostic marker for prognosis of the disease.

语种英语
WOS记录号WOS:000226719900007
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被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51894
专题基础医学院_免疫学系
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Immunol, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Peoples Hosp, Ctr Hepatobiliary Surg, Beijing 100871, Peoples R China
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Yang, XA,Dong, XY,Qiao, H,et al. Immunohistochemical analysis of the expression of FATE/BJ-HCC-2 antigen in normal and malignant tissues[J]. LABORATORY INVESTIGATION,2005,85(2):205-213.
APA Yang, XA.,Dong, XY.,Qiao, H.,Wang, YD.,Peng, JR.,...&Chen, WF.(2005).Immunohistochemical analysis of the expression of FATE/BJ-HCC-2 antigen in normal and malignant tissues.LABORATORY INVESTIGATION,85(2),205-213.
MLA Yang, XA,et al."Immunohistochemical analysis of the expression of FATE/BJ-HCC-2 antigen in normal and malignant tissues".LABORATORY INVESTIGATION 85.2(2005):205-213.
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